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Protein

Serine/threonine-protein kinase PKH2

Gene

PKH2

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is part sphingolipid-mediated signaling pathway that is required for the internalization step of endocytosis by regulating eisosome assembly and organization, and modulating the organization of the plasma membrane. Phosphorylates and activates PKC1. Activates YPK1 and YPK2, 2 components of signaling cascade required for maintenance of cell wall integrity. Required for stress-induced P-body assembly and regulates global mRNA decay at the deadenylation step.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei269ATPBy similarity1
Binding sitei325ATPBy similarity1
Active sitei364Proton acceptorPROSITE-ProRule annotation1
Binding sitei368ATP; via carbonyl oxygenBy similarity1
Binding sitei382ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 252ATPBy similarity3
Nucleotide bindingi319 – 321ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lipid binding Source: CGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PKH2By similarity (EC:2.7.11.1By similarity)
Alternative name(s):
PKB-activating kinase homolog 2By similarity
Gene namesi
Name:PKH21 Publication
Synonyms:PKH11 Publication
ORF Names:CaO19.12690, CaO19.5224, orf19.5224
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000187991. PKH2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmcell cortex By similarity

  • Note: Localizes at eisosomes, large, immobile complexes that mark sites of endocytosis near the plasma membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Sensitive to cell wall-damaging agents SDS and Congo red.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004330831 – 944Serine/threonine-protein kinase PKH2Add BLAST944

Proteomic databases

PRIDEiQ5A3P6.

Interactioni

Chemistry databases

BindingDBiQ5A3P6.

Structurei

Secondary structure

1944
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi237 – 239Combined sources3
Beta strandi240 – 248Combined sources9
Beta strandi250 – 259Combined sources10
Turni260 – 262Combined sources3
Beta strandi265 – 272Combined sources8
Helixi273 – 278Combined sources6
Helixi282 – 294Combined sources13
Beta strandi299 – 301Combined sources3
Beta strandi306 – 309Combined sources4
Beta strandi311 – 319Combined sources9
Helixi326 – 330Combined sources5
Beta strandi333 – 335Combined sources3
Helixi338 – 357Combined sources20
Beta strandi369 – 372Combined sources4
Beta strandi378 – 380Combined sources3
Helixi383 – 385Combined sources3
Beta strandi392 – 394Combined sources3
Beta strandi405 – 407Combined sources3
Helixi417 – 422Combined sources6
Helixi427 – 443Combined sources17
Beta strandi451 – 453Combined sources3
Helixi454 – 462Combined sources9
Helixi473 – 482Combined sources10
Turni487 – 489Combined sources3
Helixi493 – 497Combined sources5
Beta strandi499 – 502Combined sources4
Helixi510 – 514Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0TX-ray3.16A1-944[»]
ProteinModelPortaliQ5A3P6.
SMRiQ5A3P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini240 – 502Protein kinasePROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 316PIF-pocket1 PublicationAdd BLAST46

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.1 Publication

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ5A3V9.
KOiK06276.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5A3P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKFRYSLHQ HYSKRNSSDK SKDSPISQNS NEENDSTKLS SSSLQDLHDD
60 70 80 90 100
LDDIYNNYTL AQGTNNNSVD TLDSENNQAI NKFIDKPPAI HGMEPQLPVM
110 120 130 140 150
HVSSRLSSLG NTTNETGESI AKSAPGTPLS SHSFDFRPHH PRAVTNSSLN
160 170 180 190 200
VLLDTPNVSS EFNHLVDQTP PNESVERFDD SNNTVDNTEE EENNDDTDEI
210 220 230 240 250
PKSETLKQNE ENWEKKGAAV KTIKTMDGEM KTIRRNVTDF KFGKELGEGS
260 270 280 290 300
YSTVILATDK ITGKQYAVKV LDKRHIIKEK KVKYVNIEKH ALNRLSNRLG
310 320 330 340 350
VISLYFTFQD KDSLYFVLDY ASNGELLTLI KRYNTLNEEC TRHFGAQILD
360 370 380 390 400
AIKYMHDNGV IHRDLKPENI LLDDKMRIQI TDFGTARLLE KKNDESEEYP
410 420 430 440 450
VDVRAKSFVG TAEYVSPELL ENKYCGKPGD VWAFGCIIYQ MIAGKPPFKA
460 470 480 490 500
TNEYLTFQKI TKLQFAFSAG FPTIIRDLIK KILVLQPSRR ATIPEIQKHY
510 520 530 540 550
FFQSVDFKDF DSIWLSDPPE IGPYKMTAKS MMKVPELNKA PITTVIKKNV
560 570 580 590 600
KKSTNSNSNT NNVATAVGGS SSNGHKGSSP TPEKEPSPAT INNKSTEKVS
610 620 630 640 650
AASVAAYVLN KPATNQNSST SEDSSKRSSN SNETRKLSYS QQDYIPGTNI
660 670 680 690 700
LRPQISTRPS VGSYVKTTPS KDRKTLTKVP SNIHQQQEKV KPKVMEVKPA
710 720 730 740 750
TTLEAAWEPY LTHPDERILR IGPVIAHKEP TEPFEKKNKA SLHISPLDIN
760 770 780 790 800
KEQRSRSNTS LLTQIVNEVN NNTSELKKVE NADESLAIIE PQYNMKRSPT
810 820 830 840 850
SDSKKSMDIE RSASTSGSRI SKKAIFKKLG FSHLEKNDSE ESNGPSLTEK
860 870 880 890 900
PQTCTLVVTT HGRALLFIRN DIESNYLLIA EIKLKYPFIH FQELVISQTK
910 920 930 940
FSKLVPSVGV FVISSIDNSL IFEVEKFEVN QWTEALAKSK YNEI
Length:944
Mass (Da):106,213
Last modified:April 26, 2005 - v1
Checksum:i74517872EACE9458
GO

Sequence cautioni

The sequence EAK97360 differs from that shown. Reason: Frameshift at position 944.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti280K → Q in allele CaO19.12690. 1
Natural varianti431V → I in allele CaO19.12690. 1
Natural varianti542I → V in allele CaO19.12690. 1
Natural varianti564A → T in allele CaO19.12690. 1
Natural varianti570S → G in allele CaO19.12690. 1
Natural varianti839S → T in allele CaO19.12690. 1
Natural varianti899T → N in allele CaO19.12690. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000071 Genomic DNA. Translation: EAK97297.1.
AACQ01000070 Genomic DNA. Translation: EAK97360.1. Frameshift.
RefSeqiXP_716293.1. XM_711200.1.
XP_716356.1. XM_711263.1.

Genome annotation databases

EnsemblFungiiEAK97297; EAK97297; CaO19.5224.
EAK97360; EAK97360; CaO19.12690.
GeneIDi3641944.
3642058.
KEGGical:CaO19.12690.
cal:CaO19.5224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000071 Genomic DNA. Translation: EAK97297.1.
AACQ01000070 Genomic DNA. Translation: EAK97360.1. Frameshift.
RefSeqiXP_716293.1. XM_711200.1.
XP_716356.1. XM_711263.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0TX-ray3.16A1-944[»]
ProteinModelPortaliQ5A3P6.
SMRiQ5A3P6.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ5A3P6.

Proteomic databases

PRIDEiQ5A3P6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK97297; EAK97297; CaO19.5224.
EAK97360; EAK97360; CaO19.12690.
GeneIDi3641944.
3642058.
KEGGical:CaO19.12690.
cal:CaO19.5224.

Organism-specific databases

CGDiCAL0000187991. PKH2.

Phylogenomic databases

InParanoidiQ5A3V9.
KOiK06276.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKH2_CANAL
AccessioniPrimary (citable) accession number: Q5A3P6
Secondary accession number(s): Q5A3V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2015
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.