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Q5A1D5 (SPT16_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FACT complex subunit SPT16
Alternative name(s):
CaCDC68
Cell division control protein 68
Facilitates chromatin transcription complex subunit SPT16
Gene names
Name:SPT16
Synonyms:CDC68
ORF Names:CaO19.10402, CaO19.2884
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length1060 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II By similarity.

Subunit structure

Forms a stable heterodimer with POB3. The SPT16-POB3 dimer weakly associates with multiple molecules of NHP6 to form the FACT complex By similarity.

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Sequence similarities

Belongs to the peptidase M24 family. SPT16 subfamily.

Caution

Although related to the peptidase M24 family, this protein lacks conserved active site residues suggesting that it may lack peptidase activity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10601060FACT complex subunit SPT16
PRO_0000245181

Regions

Coiled coil508 – 53225 Potential
Compositional bias140 – 15617Asn-rich
Compositional bias982 – 9854Poly-Glu
Compositional bias987 – 104458Asp-rich (acidic)

Experimental info

Sequence conflict146 – 1494Missing in AAG48574. Ref.1
Sequence conflict3381N → S in AAG48574. Ref.1
Sequence conflict404 – 4063Missing in AAG48574. Ref.1
Sequence conflict7301P → PQS in AAG48574. Ref.1
Sequence conflict1030 – 10323Missing in AAG48574. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5A1D5 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: C12A14005E6B3E25

FASTA1,060121,378
        10         20         30         40         50         60 
MSEVNIDAGL FYKRLSIFQK QLTANNIPQA LIIVGARSDD NTYKKSTVLQ NWLLGYEFIH 

        70         80         90        100        110        120 
TAIYITDKKC IFITSEGKSK HLKHLTNQKP DLVELWIRTK DVEHNKQLFI KLLETMTKLD 

       130        140        150        160        170        180 
SKYGKILKDK YDGKFIDEWN QILNDDNNNN NNNTTNDHAL SAVDLAVTVS QALAVKDSEE 

       190        200        210        220        230        240 
FNNTKIASNA SVVMMDTFVN DMMIIVDDEK KITNSQLTDQ IEDKIENNKW YLKTKLGKNL 

       250        260        270        280        290        300 
LQSIKDFDPE YLEYCYSPII QSGGDYDLKP SAVSTDKPLI GEGVILSSIG LRYKSYCSNI 

       310        320        330        340        350        360 
ARTFLIDPTS EMETNYDFLL QLQKYIVDNL LKDGVPANKV YQDTIDYIKK ERPDLVNHFT 

       370        380        390        400        410        420 
KNCGWLLGME FRDSTFILNA KTTDRKLTTG QIISLTIGFN NLSNDKNDKN DKNDNKTNHQ 

       430        440        450        460        470        480 
KNKQTYALLL TDTIKITDDS SILLTNYSKD RAAISFSFND DNETQKENNN NNNKRPGLSQ 

       490        500        510        520        530        540 
TSNTTGLKLE STENTAILKS KLRHENTNAD DANSEKLRQE IQIKLHEKRL QEGLARFSKA 

       550        560        570        580        590        600 
DATDADDFKP IFKKYESYVR ESQIPNSVND LKIHIDYKNQ TIILPISGRP VPFHINSYKS 

       610        620        630        640        650        660 
GSQNEEGDFT YLRLNFNSPG AGGNVTKKQE LPYEDSPDNS FLRSITIRSR DRQRMVDVYK 

       670        680        690        700        710        720 
AIQDLKKDSV KREQEKKQMA DVITQANLIE LKGSRVKKLN NVFIRPTPDT KKIGGVLQIH 

       730        740        750        760        770        780 
ENGLRYQSQP QSQSNFKNDQ RVDVLFSNIK HLFFQPCKDE LIVLIHCHLK NPIMIGKRKT 

       790        800        810        820        830        840 
FDVQFYREAS DMAFDETGGR KRKYRYGDED ELQQEQEERR RKALLDKEFK GFAELIADSS 

       850        860        870        880        890        900 
HGMVDLDIPF RELGFQGVPF RSSVLCVPTR DCLVQLIDPP YLVVTLEEIE IAHLERVQFG 

       910        920        930        940        950        960 
LKNFDLVFVF KDFNKPVVHI NTIPVELLED VKSWLTDVDI PISEGQMNLN WVQIMKTVLA 

       970        980        990       1000       1010       1020 
DPYQFFIDGG WAFLTGQGES DEEEESDEES DFRVSDEDPQ DEDEESDDYA SEEESDDYSG 

      1030       1040       1050       1060 
SDDDGSGGGD DDDDDDSESG EDWDALERKA AKADRNSGFD 

« Hide

References

« Hide 'large scale' references
[1]"Validation of Cdc68p as a novel antifungal target."
Buurman E.T., Jiang W., McCoy M., Averett D.R., Thompson C.M., Wobbe C.R.
Arch. Microbiol. 178:428-436(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1006.
[2]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF253047 Genomic DNA. Translation: AAG48574.1.
AACQ01000084 Genomic DNA. Translation: EAK96517.1.
AACQ01000083 Genomic DNA. Translation: EAK96576.1.
RefSeqXP_715540.1. XM_710447.1.
XP_715596.1. XM_710503.1.

3D structure databases

ProteinModelPortalQ5A1D5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0005222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3642741.
3642787.
KEGGcal:CaO19.10402.
cal:CaO19.2884.

Organism-specific databases

CGDCAL0005222. CDC68.

Phylogenomic databases

eggNOGCOG5406.
OrthoDBEOG7SFJ58.

Family and domain databases

Gene3D3.90.230.10. 1 hit.
InterProIPR013719. DUF1747.
IPR013953. FACT_Spt16p.
IPR000994. Pept_M24_structural-domain.
[Graphical view]
PfamPF00557. Peptidase_M24. 1 hit.
PF08512. Rtt106. 1 hit.
PF08644. SPT16. 1 hit.
[Graphical view]
SUPFAMSSF55920. SSF55920. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSPT16_CANAL
AccessionPrimary (citable) accession number: Q5A1D5
Secondary accession number(s): Q9HF08
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: April 26, 2005
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Candida albicans

Candida albicans: entries and gene names