Q5A1D3 (ERK1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular signal-regulated kinase 1
Short name=ERK1
EC=2.7.11.24

Alternative name(s):
MAP kinase 1
Short name=MAPK 1

Gene names

Name:	CEK1
Synonyms:	ERK1
ORF Names:	CaO19.10404, CaO19.2886

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-231 and Tyr-233, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence EAK96519.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence EAK96578.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from genetic interaction PubMed 9596738. Source: CGD cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular response to biotic stimulus Inferred from mutant phenotype PubMed 9596738. Source: CGD cellular response to drug Inferred from mutant phenotype PubMed 17604452. Source: CGD cellular response to starvation Inferred from mutant phenotype PubMed 9398674PubMed 9596738. Source: CGD conjugation with cellular fusion Inferred from mutant phenotype PubMed 12453219. Source: CGD filamentous growth of a population of unicellular organisms in response to biotic stimulus Inferred from mutant phenotype PubMed 9596738. Source: CGD filamentous growth of a population of unicellular organisms in response to starvation Inferred from mutant phenotype PubMed 9398674PubMed 9596738. Source: CGD fungal-type cell wall biogenesis Inferred from mutant phenotype PubMed 19563901. Source: CGD fungal-type cell wall organization Inferred from mutant phenotype PubMed 16467475. Source: CGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from mutant phenotype PubMed 12381467PubMed 9596738PubMed 9741091. Source: CGD positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus Inferred from mutant phenotype PubMed 9596738. Source: CGD positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Inferred from mutant phenotype PubMed 9398674PubMed 9596738. Source: CGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC protein kinase activity Inferred from sequence or structural similarity PubMed 1409649. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Extracellular signal-regulated kinase 1		PRO_0000186325
Regions
	Domain	71 – 376	306	Protein kinase
	Nucleotide binding	77 – 85	9	ATP By similarity
	Motif	231 – 233	3	TXY
	Compositional bias	5 – 53	49	Ala/Gln-rich
Sites
	Active site	195	1	Proton acceptor By similarity
	Binding site	100	1	ATP By similarity
Amino acid modifications
	Modified residue	231	1	Phosphothreonine By similarity
	Modified residue	233	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	32 – 35	4	Missing in strain: ATCC 60193 / S-24; in allele 237 B.
	Natural variant	33	1	Q → QA in strain: R-2617; in allele 249 D.
	Natural variant	34 – 35	2	QQ → AQAQA in strain: R-2805; in allele 255 A.
	Natural variant	34	1	Q → A in strain: R-2617, R-2624, R-2777 and R-2805; in allele 246 D, 249 E, 249 C and 246 E.
	Natural variant	38 – 43	6	Missing in strain: ATCC 36232; in allele 225 A.
	Natural variant	38 – 41	4	Missing in strain: R-2621; in allele 237 A.
	Natural variant	41	1	Q → QQ in strain: R-2621; in allele 249 B.
	Natural variant	41	1	Missing in strain: R-2540; in allele 243 A.
	Natural variant	52	1	A → T in strain: R-2617; in allele 246 D.

Sequences

	Sequence		Length	Mass (Da)
	Q5A1D3 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: 471BE4423FDAAAD5	FASTA	422	48,623
	10 20 30 40 50 60 MNIDQHHQLQ QQHQQQMLQQ QAQAQAQAQA QAQQQQQQQQ QAAAAAAAAN AAATTSSSSP 70 80 90 100 110 120 RQVSFNVSDH YQILEIVGEG AYGIVCSAIH KPSQQKVAIK KIEPFERSML CLRTLRELKL 130 140 150 160 170 180 LKHFNHENII SILAIQRPIN YESFNEIYLI QELMETDLHR VIRTQNLSDD HIQYFIYQTL 190 200 210 220 230 240 RALKAMHSAN VLHRDLKPSN LLLNSNCDLK ICDFGLARSI ASQEDNYGFM TEYVATRWYR 250 260 270 280 290 300 APEIMLTFQE YTTAIDVWSV GCILAEMLSG RPLFPGRDYH NQLWLIMEVL GTPNMEDYYN 310 320 330 340 350 360 IKSKRAREYI RSLPFCKKIP FSELFANTNN NTSTSTSNTG GRTNINPLAL DLLEKLLIFN 370 380 390 400 410 420 PAKRITVEDA LKHPYLQLYH DPNDEPISDK IPEDFFDFDK MKDQLTIEDL KKLLYEEIMK PL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Sequence analysis of a compound coding-region microsatellite in Candida albicans resolves homoplasies and provides a high-resolution tool for genotyping." Metzgar D., Field D., Haubrich R., Wills C. FEMS Immunol. Med. Microbiol. 20:103-109(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58. Strain: ATCC 36232, ATCC 60193 / S-24, R-2436, R-2535, R-2540, R-2607, R-2617, R-2621, R-2624, R-2777 and R-2805.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000084 Genomic DNA. Translation: EAK96519.1. Different initiation. AACQ01000083 Genomic DNA. Translation: EAK96578.1. Different initiation. U95784 Genomic DNA. Translation: AAB88588.1. U95785 Genomic DNA. Translation: AAB88589.1. U95786 Genomic DNA. Translation: AAB88590.1. U95787 Genomic DNA. Translation: AAB88591.1. U95788 Genomic DNA. Translation: AAB88592.1. U95789 Genomic DNA. Translation: AAB88593.1. U95790 Genomic DNA. Translation: AAB88594.1. U95791 Genomic DNA. Translation: AAB88595.1. U95792 Genomic DNA. Translation: AAB88596.1. U95793 Genomic DNA. Translation: AAB88597.1. U95794 Genomic DNA. Translation: AAB88598.1. U95795 Genomic DNA. Translation: AAB88599.1. U95796 Genomic DNA. Translation: AAB88600.1. U95797 Genomic DNA. Translation: AAB88601.1. U95798 Genomic DNA. Translation: AAB88602.1.
RefSeq	XP_715542.1. XM_710449.1. XP_715598.1. XM_710505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0005224.
Proteomic databases
PRIDE	Q5A1D3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3642743. 3642789.
KEGG	cal:CaO19.10404. cal:CaO19.2886.
Organism-specific databases
CGD	CAL0005224. CEK1.
Phylogenomic databases
eggNOG	COG0515.
KO	K04371.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERK1_CANAL

Accession

Primary (citable) accession number: Q5A1D3
Secondary accession number(s): O13435 , P28869, P87079, P87080, P87081, P87082, P87083, P87084, P87085, P87086, P87322, Q6LC13

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program