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Q5A0Y8

- MGMT_CANAL

UniProt

Q5A0Y8 - MGMT_CANAL

Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGT1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine By similarity.By similarity

    Catalytic activityi

    DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei115 – 1151DNABy similarity
    Binding sitei127 – 1271DNABy similarity
    Active sitei144 – 1441Nucleophile; methyl group acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
    Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
    Short name:
    MGMT
    DNA repair MTase
    O-6-methylguanine-DNA-alkyltransferase
    Gene namesi
    Name:MGT1
    ORF Names:CaO19.10620, CaO19.3108
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0002223. orf19.3108.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 175175Methylated-DNA--protein-cysteine methyltransferasePRO_0000333682Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5476.CAL0002223.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5A0Y8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MGMT family.Curated

    Phylogenomic databases

    eggNOGiCOG0350.
    OrthoDBiEOG7BKD6H.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
    IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01035. DNA_binding_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46767. SSF46767. 1 hit.
    TIGRFAMsiTIGR00589. ogt. 1 hit.
    PROSITEiPS00374. MGMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5A0Y8-1 [UniParc]FASTAAdd to Basket

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    MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP    50
    YQLKPLSTMT GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV 100
    WKELVKIPAG QTRTYKELAD LLGTHSRVIG NCCGANRIAV LIPCHRVVGA 150
    NNKLTGYRWG KSYKEYLLKQ EGIGI 175
    Length:175
    Mass (Da):19,492
    Last modified:April 26, 2005 - v1
    Checksum:i8D9B5BF2AD259EE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000086 Genomic DNA. Translation: EAK96392.1.
    AACQ01000085 Genomic DNA. Translation: EAK96463.1.
    RefSeqiXP_715417.1. XM_710324.1.
    XP_715487.1. XM_710394.1.

    Genome annotation databases

    GeneIDi3642835.
    3642966.
    KEGGical:CaO19.10620.
    cal:CaO19.3108.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000086 Genomic DNA. Translation: EAK96392.1 .
    AACQ01000085 Genomic DNA. Translation: EAK96463.1 .
    RefSeqi XP_715417.1. XM_710324.1.
    XP_715487.1. XM_710394.1.

    3D structure databases

    ProteinModelPortali Q5A0Y8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0002223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3642835.
    3642966.
    KEGGi cal:CaO19.10620.
    cal:CaO19.3108.

    Organism-specific databases

    CGDi CAL0002223. orf19.3108.

    Phylogenomic databases

    eggNOGi COG0350.
    OrthoDBi EOG7BKD6H.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR001497. MethylDNA_cys_MeTrfase_AS.
    IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01035. DNA_binding_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46767. SSF46767. 1 hit.
    TIGRFAMsi TIGR00589. ogt. 1 hit.
    PROSITEi PS00374. MGMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.

    Entry informationi

    Entry nameiMGMT_CANAL
    AccessioniPrimary (citable) accession number: Q5A0Y8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3