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Q5A0Y8 (MGMT_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylated-DNA--protein-cysteine methyltransferase

EC=2.1.1.63
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
Gene names
Name:MGT1
ORF Names:CaO19.10620, CaO19.3108
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine By similarity.

Catalytic activity

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Subcellular location

Nucleus By similarity.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Sequence similarities

Belongs to the MGMT family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

methylated-DNA-[protein]-cysteine S-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175Methylated-DNA--protein-cysteine methyltransferase
PRO_0000333682

Sites

Active site1441Nucleophile; methyl group acceptor By similarity
Binding site1151DNA By similarity
Binding site1271DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5A0Y8 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 8D9B5BF2AD259EE7

FASTA17519,492
        10         20         30         40         50         60 
MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP YQLKPLSTMT 

        70         80         90        100        110        120 
GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV WKELVKIPAG QTRTYKELAD 

       130        140        150        160        170 
LLGTHSRVIG NCCGANRIAV LIPCHRVVGA NNKLTGYRWG KSYKEYLLKQ EGIGI 

« Hide

References

[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000086 Genomic DNA. Translation: EAK96392.1.
AACQ01000085 Genomic DNA. Translation: EAK96463.1.
RefSeqXP_715417.1. XM_710324.1.
XP_715487.1. XM_710394.1.

3D structure databases

ProteinModelPortalQ5A0Y8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0002223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3642835.
3642966.
KEGGcal:CaO19.10620.
cal:CaO19.3108.

Organism-specific databases

CGDCAL0002223. orf19.3108.

Phylogenomic databases

eggNOGCOG0350.
OrthoDBEOG7BKD6H.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMSSF46767. SSF46767. 1 hit.
TIGRFAMsTIGR00589. ogt. 1 hit.
PROSITEPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGMT_CANAL
AccessionPrimary (citable) accession number: Q5A0Y8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 26, 2005
Last modified: November 13, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names