Q5A0Y8 (MGMT_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	MGT1
ORF Names:	CaO19.10620, CaO19.3108

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine By similarity.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Subcellular location	Nucleus By similarity.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000333682
Sites
	Active site	144	1	Nucleophile; methyl group acceptor By similarity
	Binding site	115	1	DNA By similarity
	Binding site	127	1	DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5A0Y8 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 8D9B5BF2AD259EE7	FASTA	175	19,492
	10 20 30 40 50 60 MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP YQLKPLSTMT 70 80 90 100 110 120 GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV WKELVKIPAG QTRTYKELAD 130 140 150 160 170 LLGTHSRVIG NCCGANRIAV LIPCHRVVGA NNKLTGYRWG KSYKEYLLKQ EGIGI

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000086 Genomic DNA. Translation: EAK96392.1. AACQ01000085 Genomic DNA. Translation: EAK96463.1.
RefSeq	XP_715417.1. XM_710324.1. XP_715487.1. XM_710394.1.
3D structure databases
HSSP	HSSP built from PDB template 1SFE based on UniProtKB P06134.
ProteinModelPortal	Q5A0Y8.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0002223.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3642835. 3642966.
KEGG	cal:CaO19.10620. cal:CaO19.3108.
Organism-specific databases
CGD	CAL0002223. orf19.3108.
Phylogenomic databases
eggNOG	COG0350.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01035. DNA_binding_1. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGMT_CANAL

Accession

Primary (citable) accession number: Q5A0Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program