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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGT1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine (By similarity).By similarity

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151DNABy similarity
Binding sitei127 – 1271DNABy similarity
Active sitei144 – 1441Nucleophile; methyl group acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. damaged DNA binding Source: GO_Central
  2. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: GO_Central

GO - Biological processi

  1. DNA dealkylation involved in DNA repair Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:MGT1
ORF Names:CaO19.10620, CaO19.3108
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0002223. orf19.3108.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Methylated-DNA--protein-cysteine methyltransferasePRO_0000333682Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0002223.

Structurei

3D structure databases

ProteinModelPortaliQ5A0Y8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiCOG0350.
InParanoidiQ5A0Y8.
OrthoDBiEOG7BKD6H.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5A0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP
60 70 80 90 100
YQLKPLSTMT GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV
110 120 130 140 150
WKELVKIPAG QTRTYKELAD LLGTHSRVIG NCCGANRIAV LIPCHRVVGA
160 170
NNKLTGYRWG KSYKEYLLKQ EGIGI
Length:175
Mass (Da):19,492
Last modified:April 26, 2005 - v1
Checksum:i8D9B5BF2AD259EE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000086 Genomic DNA. Translation: EAK96392.1.
AACQ01000085 Genomic DNA. Translation: EAK96463.1.
RefSeqiXP_715417.1. XM_710324.1.
XP_715487.1. XM_710394.1.

Genome annotation databases

GeneIDi3642835.
3642966.
KEGGical:CaO19.10620.
cal:CaO19.3108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000086 Genomic DNA. Translation: EAK96392.1.
AACQ01000085 Genomic DNA. Translation: EAK96463.1.
RefSeqiXP_715417.1. XM_710324.1.
XP_715487.1. XM_710394.1.

3D structure databases

ProteinModelPortaliQ5A0Y8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5476.CAL0002223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3642835.
3642966.
KEGGical:CaO19.10620.
cal:CaO19.3108.

Organism-specific databases

CGDiCAL0002223. orf19.3108.

Phylogenomic databases

eggNOGiCOG0350.
InParanoidiQ5A0Y8.
OrthoDBiEOG7BKD6H.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiMGMT_CANAL
AccessioniPrimary (citable) accession number: Q5A0Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 26, 2005
Last modified: February 4, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.