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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGT1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.By similarity

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115DNABy similarity1
Binding sitei127DNABy similarity1
Active sitei144Nucleophile; methyl group acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:MGT1
Ordered Locus Names:CAALFM_C407010CA
ORF Names:CaO19.10620, CaO19.3108
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 4

Organism-specific databases

CGDiCAL0000196232 orf19.3108

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003336821 – 175Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST175

Structurei

3D structure databases

ProteinModelPortaliQ5A0Y8
SMRiQ5A0Y8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

InParanoidiQ5A0Y8
KOiK00567
OMAiGNCCGAN
OrthoDBiEOG092C4PPW

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

Q5A0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP
60 70 80 90 100
YQLKPLSTMT GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV
110 120 130 140 150
WKELVKIPAG QTRTYKELAD LLGTHSRVIG NCCGANRIAV LIPCHRVVGA
160 170
NNKLTGYRWG KSYKEYLLKQ EGIGI
Length:175
Mass (Da):19,492
Last modified:April 26, 2005 - v1
Checksum:i8D9B5BF2AD259EE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017626 Genomic DNA Translation: AOW29422.1
RefSeqiXP_715417.1, XM_710324.1

Genome annotation databases

EnsemblFungiiAOW29422; AOW29422; CAALFM_C407010CA
GeneIDi3642966
KEGGical:CAALFM_C407010CA

Similar proteinsi

Entry informationi

Entry nameiMGMT_CANAL
AccessioniPrimary (citable) accession number: Q5A0Y8
Secondary accession number(s): A0A1D8PMQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 26, 2005
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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