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Q5A0Y8

- MGMT_CANAL

UniProt

Q5A0Y8 - MGMT_CANAL

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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene
MGT1, CaO19.10620, CaO19.3108
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine By similarity.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151DNA By similarity
Binding sitei127 – 1271DNA By similarity
Active sitei144 – 1441Nucleophile; methyl group acceptor By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:MGT1
ORF Names:CaO19.10620, CaO19.3108
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0002223. orf19.3108.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Methylated-DNA--protein-cysteine methyltransferasePRO_0000333682Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0002223.

Structurei

3D structure databases

ProteinModelPortaliQ5A0Y8.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.

Phylogenomic databases

eggNOGiCOG0350.
OrthoDBiEOG7BKD6H.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5A0Y8-1 [UniParc]FASTAAdd to Basket

« Hide

MSNLYYTVFD TDVCTVLLVL TLNGFVCYAS LGKPAIELKG IMAKDFSSLP    50
YQLKPLSTMT GDKIEIDKSV EKFKLLVETP SVSQDIKTEL LFGTPLQRKV 100
WKELVKIPAG QTRTYKELAD LLGTHSRVIG NCCGANRIAV LIPCHRVVGA 150
NNKLTGYRWG KSYKEYLLKQ EGIGI 175
Length:175
Mass (Da):19,492
Last modified:April 26, 2005 - v1
Checksum:i8D9B5BF2AD259EE7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000086 Genomic DNA. Translation: EAK96392.1.
AACQ01000085 Genomic DNA. Translation: EAK96463.1.
RefSeqiXP_715417.1. XM_710324.1.
XP_715487.1. XM_710394.1.

Genome annotation databases

GeneIDi3642835.
3642966.
KEGGical:CaO19.10620.
cal:CaO19.3108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000086 Genomic DNA. Translation: EAK96392.1 .
AACQ01000085 Genomic DNA. Translation: EAK96463.1 .
RefSeqi XP_715417.1. XM_710324.1.
XP_715487.1. XM_710394.1.

3D structure databases

ProteinModelPortali Q5A0Y8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0002223.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3642835.
3642966.
KEGGi cal:CaO19.10620.
cal:CaO19.3108.

Organism-specific databases

CGDi CAL0002223. orf19.3108.

Phylogenomic databases

eggNOGi COG0350.
OrthoDBi EOG7BKD6H.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01035. DNA_binding_1. 1 hit.
[Graphical view ]
SUPFAMi SSF46767. SSF46767. 1 hit.
TIGRFAMsi TIGR00589. ogt. 1 hit.
PROSITEi PS00374. MGMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiMGMT_CANAL
AccessioniPrimary (citable) accession number: Q5A0Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 26, 2005
Last modified: November 13, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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