Q5A0M4 (EF2_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 2
Short name=EF-2

Gene names

Name:	EFT2
ORF Names:	CaO19.13210, CaO19.5788

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity.
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Has antigenic properties.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Sequence caution	The sequence EAK96235.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAK96302.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to drug Inferred from direct assay PubMed 10428815. Source: CGD
Cellular_component	cell surface Inferred from direct assay PubMed 12782322. Source: CGD plasma membrane Inferred from direct assay PubMed 19824013. Source: CGD ribosome Inferred from sequence or structural similarity PubMed 10095118. Source: CGD yeast-form cell wall Inferred from direct assay PubMed 12782322. Source: CGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 10428815. Source: CGD drug binding Inferred from direct assay PubMed 11402051. Source: CGD translation elongation factor activity Inferred from mutant phenotype PubMed 10095118. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	Elongation factor 2		PRO_0000091015
Regions
	Nucleotide binding	26 – 33	8	GTP By similarity
	Nucleotide binding	104 – 108	5	GTP By similarity
	Nucleotide binding	158 – 161	4	GTP By similarity
Amino acid modifications
	Modified residue	699	1	Diphthamide By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5A0M4 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: 5C8C740109A23189	FASTA	842	93,354
	10 20 30 40 50 60 MVAFTIEQIR GLMDKVTNVR NMSVIAHVDH GKSTLSDSLV QKAGIISAAK AGDARFMDTR 70 80 90 100 110 120 KDEQERGITI KSTAISLYAS MTDEDVKDIK QKTDGNSFLV NLIDSPGHVD FSSEVTAALR 130 140 150 160 170 180 VTDGALVVVD TVEGVCVQTE TVLRQALGER IKPVVVINKV DRALLELQTT KEDLYQTFAR 190 200 210 220 230 240 TVESVNVIIS TYCDPVLGDV QVYPQKGTVA FASGLHGWAF TVRQFANKYS KKFGVDKEKM 250 260 270 280 290 300 MERLWGDSYF NPKTKKWTNK DKDADGKPLE RAFNMFILDP IFRLFAAIMN FKKDEIPVLL 310 320 330 340 350 360 EKLEIQLKGD EKDLEGKALL KVVMRKFLPA ADALLEMIVL HLPSPVTAQA YRAETLYEGP 370 380 390 400 410 420 SDDPFCTAIR NCDPNADLML YVSKMVPTSD KGRFYAFGRV FAGTVKSGQK VRIQGPNYQV 430 440 450 460 470 480 GKKEDLFLKS IQRTVLMMGR SVEQIDDCPA GNIIGLVGID QFLLKSGTIT TNEAAHNMKV 490 500 510 520 530 540 MKFSVSPVVQ VAVEVKNAND LPKLVEGLKR LSKSDPCVLT YMSESGEHIV AGTGELHLEI 550 560 570 580 590 600 CLQDLENDHA GVPLRISPPV VSYRETVEGE SSMVALSKSP NKHNRIYVKA QPIDEEVSLD 610 620 630 640 650 660 IENGVINPRD DFKARARILA DKHGWDVVDA RKIWCFGPDG NGPNLVVDQT KAVQYLNEIK 670 680 690 700 710 720 DSVVAAFQWA TKEGPIFGEN CRSVRVNILD VTLHADAIHR GGGQIIPTMR RVTYASMLLA 730 740 750 760 770 780 EPAIQEPVFL VEIQCPENAI GGIYSVLNKK RGQVISEEQR PGTPLFTVKA YLPVNESFGF 790 800 810 820 830 840 TGELRQATGG QAFPQLIFDH WQVMSGDVTD ENSKPGAIVK EKRVRAGLKP EVPEYTEYYD KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Capa L., Mendoza A., Serramia M.J., Garcia-Bustos J.F. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10261 / CBS 2718 / NBRC 1061.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[3]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 559-564; 581-585 AND 675-682, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314 / ATCC MYA-2876. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09664 Genomic DNA. Translation: CAA70857.2. AACQ01000088 Genomic DNA. Translation: EAK96235.1. Sequence problems. AACQ01000087 Genomic DNA. Translation: EAK96302.1. Sequence problems.
RefSeq	XP_715264.1. XM_710171.1. XP_715329.1. XM_710236.1.
3D structure databases
ProteinModelPortal	Q5A0M4.
SMR	Q5A0M4. Positions 1-842.
ModBase	Search...
Proteomic databases
PRIDE	Q5A0M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3642998. 3643055.
KEGG	cal:CaO19.13210. cal:CaO19.5788.
Organism-specific databases
CGD	CAL0001544. EFT2. CAL0064595. EFT2.
Phylogenomic databases
KO	K03234.
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.70.240. 1 hit.
InterPro	IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EF2_CANAL

Accession

Primary (citable) accession number: Q5A0M4
Secondary accession number(s): O13430, Q9P4S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	May 1, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program