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Q5A0M4

- EF2_CANAL

UniProt

Q5A0M4 - EF2_CANAL

Protein

Elongation factor 2

Gene

EFT2

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338GTPBy similarity
    Nucleotide bindingi104 – 1085GTPBy similarity
    Nucleotide bindingi158 – 1614GTPBy similarity

    GO - Molecular functioni

    1. drug binding Source: CGD
    2. GTPase activity Source: CGD
    3. GTP binding Source: UniProtKB-KW
    4. translation elongation factor activity Source: CGD

    GO - Biological processi

    1. cellular response to drug Source: CGD
    2. GTP catabolic process Source: GOC
    3. translational elongation Source: CGD

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 2
    Short name:
    EF-2
    Gene namesi
    Name:EFT2
    ORF Names:CaO19.13210, CaO19.5788
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0001544. EFT2.
    CAL0064595. EFT2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cell surface Source: CGD
    2. membrane Source: CGD
    3. plasma membrane Source: CGD
    4. ribosome Source: CGD
    5. yeast-form cell wall Source: CGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 842842Elongation factor 2PRO_0000091015Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei699 – 6991DiphthamideBy similarity

    Proteomic databases

    PRIDEiQ5A0M4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5A0M4.
    SMRiQ5A0M4. Positions 1-842.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 346330tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK03234.
    OrthoDBiEOG7966SQ.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5A0M4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAFTIEQIR GLMDKVTNVR NMSVIAHVDH GKSTLSDSLV QKAGIISAAK    50
    AGDARFMDTR KDEQERGITI KSTAISLYAS MTDEDVKDIK QKTDGNSFLV 100
    NLIDSPGHVD FSSEVTAALR VTDGALVVVD TVEGVCVQTE TVLRQALGER 150
    IKPVVVINKV DRALLELQTT KEDLYQTFAR TVESVNVIIS TYCDPVLGDV 200
    QVYPQKGTVA FASGLHGWAF TVRQFANKYS KKFGVDKEKM MERLWGDSYF 250
    NPKTKKWTNK DKDADGKPLE RAFNMFILDP IFRLFAAIMN FKKDEIPVLL 300
    EKLEIQLKGD EKDLEGKALL KVVMRKFLPA ADALLEMIVL HLPSPVTAQA 350
    YRAETLYEGP SDDPFCTAIR NCDPNADLML YVSKMVPTSD KGRFYAFGRV 400
    FAGTVKSGQK VRIQGPNYQV GKKEDLFLKS IQRTVLMMGR SVEQIDDCPA 450
    GNIIGLVGID QFLLKSGTIT TNEAAHNMKV MKFSVSPVVQ VAVEVKNAND 500
    LPKLVEGLKR LSKSDPCVLT YMSESGEHIV AGTGELHLEI CLQDLENDHA 550
    GVPLRISPPV VSYRETVEGE SSMVALSKSP NKHNRIYVKA QPIDEEVSLD 600
    IENGVINPRD DFKARARILA DKHGWDVVDA RKIWCFGPDG NGPNLVVDQT 650
    KAVQYLNEIK DSVVAAFQWA TKEGPIFGEN CRSVRVNILD VTLHADAIHR 700
    GGGQIIPTMR RVTYASMLLA EPAIQEPVFL VEIQCPENAI GGIYSVLNKK 750
    RGQVISEEQR PGTPLFTVKA YLPVNESFGF TGELRQATGG QAFPQLIFDH 800
    WQVMSGDVTD ENSKPGAIVK EKRVRAGLKP EVPEYTEYYD KL 842
    Length:842
    Mass (Da):93,354
    Last modified:October 19, 2011 - v2
    Checksum:i5C8C740109A23189
    GO

    Sequence cautioni

    The sequence EAK96235.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAK96302.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09664 Genomic DNA. Translation: CAA70857.2.
    AACQ01000088 Genomic DNA. Translation: EAK96235.1. Sequence problems.
    AACQ01000087 Genomic DNA. Translation: EAK96302.1. Sequence problems.
    RefSeqiXP_715264.1. XM_710171.1.
    XP_715329.1. XM_710236.1.

    Genome annotation databases

    GeneIDi3642998.
    3643055.
    KEGGical:CaO19.13210.
    cal:CaO19.5788.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09664 Genomic DNA. Translation: CAA70857.2 .
    AACQ01000088 Genomic DNA. Translation: EAK96235.1 . Sequence problems.
    AACQ01000087 Genomic DNA. Translation: EAK96302.1 . Sequence problems.
    RefSeqi XP_715264.1. XM_710171.1.
    XP_715329.1. XM_710236.1.

    3D structure databases

    ProteinModelPortali Q5A0M4.
    SMRi Q5A0M4. Positions 1-842.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5A0M4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3642998.
    3643055.
    KEGGi cal:CaO19.13210.
    cal:CaO19.5788.

    Organism-specific databases

    CGDi CAL0001544. EFT2.
    CAL0064595. EFT2.

    Phylogenomic databases

    KOi K03234.
    OrthoDBi EOG7966SQ.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Capa L., Mendoza A., Serramia M.J., Garcia-Bustos J.F.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10261 / CBS 2718 / NBRC 1061.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    3. "Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies."
      Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.
      Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 559-564; 581-585 AND 675-682, SUBCELLULAR LOCATION, ANTIGENICITY.
      Strain: SC5314 / ATCC MYA-2876.
      Tissue: Protoplast.

    Entry informationi

    Entry nameiEF2_CANAL
    AccessioniPrimary (citable) accession number: Q5A0M4
    Secondary accession number(s): O13430, Q9P4S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has antigenic properties.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3