Q5A0L9 (SMP3_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 4
EC=2.4.1.-

Alternative name(s):
CaSMP3
GPI mannosyltransferase IV
Short name=GPI-MT-IV

Gene names

Name:	SMP3
ORF Names:	CaO19.13214, CaO19.5792

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-1,2-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is essential in fungi By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Miscellaneous	May be used as a target for the development of some new fungicide, due the fact the presence of a fourth mannose in GPI-anchor proteins is essential for viability in fungi but not in mammals.
Sequence similarities	Belongs to the glycosyltransferase 22 family. PIGZ subfamily.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GPI anchor biosynthetic process Inferred from mutant phenotype Ref.2. Source: CGD fungal-type cell wall biogenesis Inferred from mutant phenotype Ref.2. Source: CGD
Cellular_component	integral to membrane Inferred from sequence or structural similarity Ref.2. Source: CGD intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-1,2-mannosyltransferase activity Inferred from mutant phenotype Ref.2. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	GPI mannosyltransferase 4		PRO_0000246274
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	282 – 302	21	Helical; Potential
	Transmembrane	310 – 330	21	Helical; Potential
	Transmembrane	332 – 348	17	Helical; Potential
	Transmembrane	350 – 370	21	Helical; Potential
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	473	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5A0L9 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 8533C495219CCD22	FASTA	498	57,985
	10 20 30 40 50 60 MIKINFNWRT FYLLTIVFRF VFTLSDSYIH PDEHFQSLEV LTNRILNYST NIPWEFQDDP 70 80 90 100 110 120 ARSLAPLYFI YGPLLYFIKF FKLNLTALQI WYIARLQISI LSWIITDFCL YWMLPSKPER 130 140 150 160 170 180 IKAIFFTSTS YITLVYQNHL FSNSIETLLL LVTILLIDDL RYVQESKDQD VQNLNKNKNL 190 200 210 220 230 240 FYTGVLISLG IFNRITFPAF LILPGWFVMK YVLKHYVSGL YLVMGFFSTT ALLILVDTIL 250 260 270 280 290 300 FGNINNVVAE PFNISSYIIA PLNNLLYNAR YENLAQHGIH PYYTHILVNM PQILGPGLIF 310 320 330 340 350 360 FVSKSYTKTT PFLTVISGLL FLSVIPHQEL RFLIPLLPLA CCSFDFTLKW VQPWMLYTWY 370 380 390 400 410 420 IFNIFMSILM GKLHQGGVVP VLDHIKSEAS VQVWWRTYTP PSWILGSNST ETTHLGEKLN 430 440 450 460 470 480 DNKFINIVDC MGADSKEVQQ ILQTISTNKP VYLITPIASF KHFDESRFSP VWNYTFHLDL 490 DHLDFADIQP GLGVYQLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Deficiencies in the essential Smp3 mannosyltransferase block glycosylphosphatidylinositol assembly and lead to defects in growth and cell wall biogenesis in Candida albicans." Grimme S.J., Colussi P.A., Taron C.H., Orlean P. Microbiology 150:3115-3128(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000088 Genomic DNA. Translation: EAK96239.1. AACQ01000087 Genomic DNA. Translation: EAK96306.1.
RefSeq	XP_715268.1. XM_710175.1. XP_715333.1. XM_710240.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0001551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3642980. 3643037.
KEGG	cal:CaO19.13214. cal:CaO19.5792.
Organism-specific databases
CGD	CAL0001551. SMP3.
Phylogenomic databases
eggNOG	NOG310998.
KO	K08098.
Enzyme and pathway databases
UniPathway	UPA00196.
Family and domain databases
InterPro	IPR005599. GPI_mannosylTrfase. [Graphical view]
PANTHER	PTHR22760. PTHR22760. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMP3_CANAL

Accession

Primary (citable) accession number: Q5A0L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program