Reviewed, UniProtKB/Swiss-Prot P52496 (DNLI4_CANAL)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
      Short name=CaLIG4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Synonyms:	CDC9
ORF Names:	CaO19.5798, CaO19.13220

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	928 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	Nucleus.
Induction	Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 928	928	DNA ligase 4		PRO_0000059584
Regions
	Domain	673 – 769	97	BRCT 1
	Domain	821 – 927	107	BRCT 2
Sites
	Active site	304	1	N6-AMP-lysine intermediate By similarity
Experimental info
	Sequence conflict	531 – 534	4	VYYS → FIIV in CAA64457. Ref.1
	Sequence conflict	564	1	A → S in CAA64457. Ref.1
	Sequence conflict	698	1	T → R in CAA64457. Ref.1
	Sequence conflict	894	1	E → K in CAA64457. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52496-1 [UniParc]. Last modified September 2, 2008. Version 3. Checksum: E15D5151593E462E	FASTA	928	107,997
	10 20 30 40 50 60 MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA FIIKTFINTF 70 80 90 100 110 120 RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK IPKESEDYIT LHDWNKSYQR 130 140 150 160 170 180 SRRFSIDEKK IRDLPLQASR IISKRRPIVD KLEEYTVPQI NSSLDQLALE KVSQGQIDIL 190 200 210 220 230 240 KPLFDNLSIP EVRWLIHILL NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP 250 260 270 280 290 300 DLRLDPSQLA IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 310 320 330 340 350 360 IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF AGNIQSVILD 370 380 390 400 410 420 GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ TAYPFFLVFD ILFLNGKDLT 430 440 450 460 470 480 NYPLFFRKNI LNRILRPIPN RFEVLDTRLG SSSEDIERAI REVVSSRCEG LVLKNVQSKY 490 500 510 520 530 540 EIDGFRNPDW IKVKPEYLEK FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN 550 560 570 580 590 600 GIEIEEFDKI ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 610 620 630 640 650 660 AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT ALGKKREPVY 670 680 690 700 710 720 SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE EMKAMVKQYG GKIVNSVDLA 730 740 750 760 770 780 TNYQIMVITE RELPVSSQYL SKGIDLVKPI WIYECIKRGC VLQLEPYFIF ASKNWDNFNH 790 800 810 820 830 840 MVDQYGDSYI IHHPLNIVVP KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA 850 860 870 880 890 900 RFLKERIERF SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 910 920 IPHFVTEAFV QASIKMNYIP DPDDYKFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Candida albicans gene encoding a DNA ligase." Andaluz E., Larriba G., Calderone R. Yeast 12:893-898(1996) [PubMed: 8840507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 56884 / 366.
[2]	Larriba G. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.
[4]	"Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans." Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G. Yeast 15:1199-1210(1999) [PubMed: 10487922] [Abstract] Cited for: FUNCTION, INDUCTION.
[5]	"Phenotypic analysis and virulence of Candida albicans LIG4 mutants." Andaluz E., Calderone R., Reyes G., Larriba G. Infect. Immun. 69:137-147(2001) [PubMed: 11119499] [Abstract] Cited for: FUNCTION.
[6]	"An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans." Andaluz E., Ciudad T., Larriba G. FEMS Yeast Res. 2:341-348(2002) [PubMed: 12702284] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95001 Genomic DNA. Translation: CAA64457.2. AACQ01000088 Genomic DNA. Translation: EAK96245.1. AACQ01000087 Genomic DNA. Translation: EAK96312.1.
RefSeq	XP_715274.1. XP_715339.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3642986. 3643043.
Organism-specific databases
CGD	CAL0001558. LIG4.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 2 hits. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_CANAL

Accession

Primary (citable) accession number: P52496
Secondary accession number(s): Q5A0L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 2, 2008
Last modified:	November 25, 2008
This is version 52 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)