Reviewed, UniProtKB/Swiss-Prot Q59YV2 (ALG10_CANAL)

Names and origin

Protein names

Recommended name:
    Alpha-1,2 glucosyltransferase ALG10
    EC=2.4.1.-
Alternative name(s):
    Alpha-2-glucosyltransferase ALG10
    Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10
    Asparagine-linked glycosylation protein 10

Gene names

Name:	ALG10
Synonyms:	DIE2
ORF Names:	CaO19.6971

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ALG10 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Alpha-1,2 glucosyltransferase ALG10		PRO_0000215452
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	158 – 178	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	243 – 263	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	312 – 332	21	Potential
	Transmembrane	357 – 377	21	Potential
	Transmembrane	384 – 404	21	Potential
	Transmembrane	429 – 449	21	Potential
	Compositional bias	95 – 101	7	Poly-Gly
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q59YV2-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 713698120987655A	FASTA	450	52,530
	10 20 30 40 50 60 MPLIIPSNDI YISIISKYVA IVIFLIFVII MFNNITHHLT QPYIDEIFHL RQCQTYCQYN 70 80 90 100 110 120 FHHWDNKITT PPGLYILGFI YSEGIKILTR SSSTGGGGGG GHLTCFNDNV LRSINLIGGV 130 140 150 160 170 180 VILPRILQQF HNGWSKNSKN QFFWSINIIS QPLLFTYYFL FYTDVSSTIL IILSLGLINY 190 200 210 220 230 240 KLLQYPMLSA LVGFMSLWFR QTNIIWIAFI ASIFIDRQIK IKTGVIDRIR QFIMKSLTNW 250 260 270 280 290 300 NKLLGYIVNI ILFVIFLKLN GGITLGDNDN HQIELHIVQV FYCFTFITFF TIPNWLNKST 310 320 330 340 350 360 IKKYYNFIIN HIILNLVIGL IIWYIMENFT IVHPFLLADN RHYAFYIYKR LLSQSYLKPL 370 380 390 400 410 420 ILMAYHFSSF QIISSLIKGG QLSFIGIFSY LIAVGLTLIP SPLFEPRYYI TPLIIFNLYI 430 440 450 NHPHNLLEFI WLNSINLITS YIFLHKGIIW

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACQ01000098 Genomic DNA. Translation: EAK95630.1.
RefSeq	XP_714677.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3643674.
KEGG	cal:CaO19.6971.
Organism-specific databases
CGD	CAL0000363. DIE2.
Phylogenomic databases
OrthoDB	EOG9FN615
Family and domain databases
InterPro	IPR016900. Alpha1_2_glucosyltferase_Alg10. IPR007006. Glycosyltransferase_ALG10. [Graphical view]
PANTHER	PTHR12989. DIE2_ALG10. 1 hit.
Pfam	PF04922. DIE2_ALG10. 1 hit. [Graphical view]
PIRSF	PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALG10_CANAL

Accession

Primary (citable) accession number: Q59YV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	April 26, 2005
Last modified:	November 24, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)