Q59W50 (CWC2_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor CWC2

Gene names

Name:	CWC2
ORF Names:	CaO19.674, CaO19.8291

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle By similarity.
Subunit structure	Associated with the spliceosome By similarity.
Subcellular location	Nucleus By similarity.
Domain	The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding By similarity.
Sequence similarities	Belongs to the RRM CWC2 family. Contains 1 C3H1-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Cell cycle mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW mRNA cis splicing, via spliceosome Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of RNA splicing Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell cycle Inferred from sequence or structural similarity. Source: UniProtKB spliceosomal snRNP assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Prp19 complex Inferred from sequence or structural similarity. Source: UniProtKB spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	U6 snRNA binding Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro pre-mRNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Pre-mRNA-splicing factor CWC2		PRO_0000081541
Regions
	Domain	141 – 217	77	RRM
	Zinc finger	78 – 105	28	C3H1-type

Sequences

	Sequence		Length	Mass (Da)
	Q59W50 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 4AFFBB5543523293	FASTA	363	41,272
	10 20 30 40 50 60 MSRKVSISSL TLSEPMTKPA RVQVNPEDID DLDKTQQVNN HQYNIWYSKS LSDANNKSTT 70 80 90 100 110 120 KSKYRVRIST DQGFTKANKN THICLFFSRG CCYLGSKCQY FHRLPKESDN FKPTQDCFGR 130 140 150 160 170 180 DKTANYRDDM DGVGSLNKVN CTLYVGGIHI KPNTEQLLVK NFQEFGTVEK VRVLQGKGCA 190 200 210 220 230 240 FVTMKTENQA QFAKEAMQSQ SLVEGSNEVL YVRWANEDKN PAAQKQEKKR QQELAYDTVK 250 260 270 280 290 300 QLLEQESNKK PRLENGAVNR IETINIDDES EDEGAVEDDE ADDDIEETRV NSLSAGVKKN 310 320 330 340 350 360 VSTHQANGTP ESSSTSDEND HNTILRNSLL KDISRLKKKM VYKREEPYLT NLLVDYSSDE DDD

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000117 Genomic DNA. Translation: EAK94705.1. AACQ01000116 Genomic DNA. Translation: EAK94746.1.
RefSeq	XP_713807.1. XM_708714.1. XP_713847.1. XM_708754.1.
3D structure databases
ProteinModelPortal	Q59W50.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0001095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3644493. 3644554.
KEGG	cal:CaO19.674. cal:CaO19.8291.
Organism-specific databases
CGD	CAL0001095. orf19.674.
Phylogenomic databases
eggNOG	NOG288665.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR000571. Znf_CCCH. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. PS50103. ZF_C3H1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CWC2_CANAL

Accession

Primary (citable) accession number: Q59W50

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program