Q59UG3 (ATG4_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine protease ATG4
EC=3.4.22.-

Alternative name(s):
Autophagy-related protein 4

Gene names

Name:	ATG4
ORF Names:	CaO19.2401, CaO19.9938

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probable cysteine protease necessary for autophagy and cytoplasm to vacuole transport (Cvt) By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C54 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Cysteine protease ATG4		PRO_0000215858
Sites
	Active site	145	1	Nucleophile By similarity
	Active site	314	1	Potential
	Active site	316	1	By similarity
Natural variations
	Natural variant	16	1	S → L in allele CaO19.9938.
	Natural variant	176	1	D → G in allele CaO19.9938.
	Natural variant	216 – 217	2	AS → TN in allele CaO19.9938.
	Natural variant	225	1	I → L in allele CaO19.9938.
	Natural variant	229	1	K → R in allele CaO19.9938.
	Natural variant	255	1	N → S in allele CaO19.9938.
	Natural variant	364 – 369	6	TSCLDI → SSCIDN in allele CaO19.9938.

Sequences

	Sequence		Length	Mass (Da)
	Q59UG3 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 9DE4FEB70E250886	FASTA	446	50,455
	10 20 30 40 50 60 MNQPNPNKQP IVQSTSEQTS NEEVDTVLGR FTLFVKDLSN GLNGSQEIPP SQESVSEEAE 70 80 90 100 110 120 VISRKIIVLG QTFDNFDNAN DYIESKLWLS YRCGFEPIPK SIDGPQPIQF FPSIIFNRST 130 140 150 160 170 180 IYSNFANLKS LFDKENFTSD AGWGCMIRTS QNLLANTLLK LYPKNEPEIV KLFQDDTSSP 190 200 210 220 230 240 FSIHNFIRVA SLSPLHVKPG EWFGPNAASL SIKRLASELL QDQEIDGIKI PRVFISENSD 250 260 270 280 290 300 LFDDEIRDVF AKEKNASVLI LFPIRLGIDK VNSYYYNSIF HLLASKYSCG IAGGKPSSSF 310 320 330 340 350 360 YFLGYEDTDL IYFDPHLPQV VETPINMDSY HTTNYNRLNI SLLDPSMMIG ILVTNIDEYI 370 380 390 400 410 420 DFKTSCLDIN NKIVHFHPHT LPVQQDSIIN QSWEEVQDEE EEFINLNVSK IENEQQQEQG 430 440 QSTDAPDEFI DIGNQSSSVV SVPSNV

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000131 Genomic DNA. Translation: EAK94117.1. AACQ01000130 Genomic DNA. Translation: EAK94169.1.
RefSeq	XP_713234.1. XM_708141.1. XP_713286.1. XM_708193.1.
3D structure databases
ProteinModelPortal	Q59UG3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q59UG3.
Protein family/group databases
MEROPS	C54.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3645061. 3645128.
KEGG	cal:CaO19.2401. cal:CaO19.9938.
Organism-specific databases
CGD	CAL0001444. orf19.2401.
Phylogenomic databases
PhylomeDB	Q59UG3.
Family and domain databases
InterPro	IPR005078. Peptidase_C54. [Graphical view]
KO	K08342.
PANTHER	PTHR22624. Peptidase_C54. 1 hit.
Pfam	PF03416. Peptidase_C54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG4_CANAL

Accession

Primary (citable) accession number: Q59UG3
Secondary accession number(s): Q59UL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program