Reviewed, UniProtKB/Swiss-Prot Q59TD3 (MED8_CANAL)

Names and origin

Protein names

Recommended name:
    Mediator of RNA polymerase II transcription subunit 8
Alternative name(s):
    Mediator complex subunit 8

Gene names

Name:	MED8
ORF Names:	CaO19.4497, CaO19.11973

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.
Subunit structure	Component of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 8 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Activator
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mediator complex Inferred from electronic annotation. Source: InterPro
Molecular function	RNA polymerase II transcription mediator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Mediator of RNA polymerase II transcription subunit 8		PRO_0000304538
Regions
	Coiled coil	30 – 59	30	Potential
Natural variations
	Natural variant	121	1	R → K in allele CaO19.4497.

Sequences

	Sequence		Length	Mass (Da)
	Q59TD3-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: EFDF7C7331CBB019	FASTA	239	27,959
	10 20 30 40 50 60 MSQTPFNPNS PSVPTTTSLP STDFSQIPYD VLESIRNRLN QIRQSLTKLA DQINNHNRHP 70 80 90 100 110 120 QKIKLPSYSY FQNQFQVLIT QLMSITNQLY NNEDLLRNTN VYPTPIFPTS AHENMLTTLL 130 140 150 160 170 180 RKKVAPEVDE WINGAKLIME NETKNKLDNQ QVEIDILKID QWVQWCLAKI QDLRDEFQFY 190 200 210 220 230 GFHTVEELDH METPQGKQEI KEKKDKEFER EQIELKITAG GQKSLHPNQV LKFMCQGQI

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000142 Genomic DNA. Translation: EAK93723.1. AACQ01000141 Genomic DNA. Translation: EAK93757.1.
RefSeq	XP_712891.1. XP_712923.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3645446. 3645495.
KEGG	cal:CaO19.11973. cal:CaO19.4497.
Organism-specific databases
CGD	CAL0079252. MED8.
Phylogenomic databases
OMA	LPEVDEW.
OrthoDB	EOG9G1NZV.
Family and domain databases
InterPro	IPR020178. Mediatior_Med8_fun. [Graphical view]
ProDom	PD114219. Mediatior_Med8_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MED8_CANAL

Accession

Primary (citable) accession number: Q59TD3
Secondary accession number(s): Q59TG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 26, 2005
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)