Q59SI2 (TIM14_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM14

Alternative name(s):
Presequence translocated-associated motor subunit PAM18

Gene names

Name:	PAM18
Synonyms:	TIM14
ORF Names:	CaO19.11667, CaO19.4190

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1) By similarity.
Subunit structure	Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 By similarity.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein By similarity.
Domain	The J domain is essential for co-chaperone activity and mediates the heterodimerization with the J-like domain of PAM16 By similarity.
Sequence similarities	Belongs to the TIM14 family. Contains 1 J domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Mitochondrial import inner membrane translocase subunit TIM14		PRO_0000071109
Regions
	Topological domain	1 – 56	56	Mitochondrial intermembrane Potential
	Transmembrane	57 – 74	18	Helical; Potential
	Topological domain	75 – 157	83	Mitochondrial matrix Potential
	Domain	99 – 157	59	J

Sequences

	Sequence		Length	Mass (Da)
	Q59SI2 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 764D3BD9383B7366	FASTA	157	17,297
	10 20 30 40 50 60 MAPLEAPILA IPGENNTHQQ QQQQFQYTGQ LQKKKAAEGS IEWYFDQTIN FMGDHPVITG 70 80 90 100 110 120 IGAFAVAYFA AGFIKSNQPG INGKAFVKGG FGAKMTAKEA LQILNLKETN LSKLKLKEQH 130 140 150 RKLMMANHPD KGGSSYIATK INEAKDFLDK RGGMKPK

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000151 Genomic DNA. Translation: EAK93469.1. AACQ01000146 Genomic DNA. Translation: EAK93624.1.
RefSeq	XP_712644.1. XM_707551.1. XP_712795.1. XM_707702.1.
3D structure databases
ProteinModelPortal	Q59SI2.
SMR	Q59SI2. Positions 85-152.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0005879.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3645592. 3645747.
KEGG	cal:CaO19.11667. cal:CaO19.4190.
Organism-specific databases
CGD	CAL0005879. orf19.4190.
Phylogenomic databases
eggNOG	COG2214.
KO	K09539.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR001623. DnaJ_domain. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIM14_CANAL

Accession

Primary (citable) accession number: Q59SI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program