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Protein

N-acetylglucosamine kinase 1

Gene

HXK1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the N-acetylglucosamine catabolic cascade that phosphorylates N-acetylglucosamine (GlcNAc), and allows the unique ability to utilise GlcNAc as carbon source. Converts GlcNAc to GlcNAc-6-P. Also able to phosphorylate glucose, glucosamine (GlcN), and mannose. Galactose, fructose, N-acetylmannosamine (ManNAc), mannosamine (ManN), galactosamine (GalN), and N-acetylgalactosamine (GalNAc) are not phosphorylated by HXK1. GlcNAc metabolism is closely associated with virulence and morphogenesis, and is involved in the cell wall synthesis. Acts both as a repressor and an activator of genes involved in maintaining cellular homeostasis. Contributes to white-opaque morphological transition and plays a role as a filamentation repressor.7 Publications

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Kineticsi

  1. KM=375.5 µM for N-acetylglucosamine (GlcNAc)1 Publication
  2. KM=482.5 µM for glucose1 Publication
  3. KM=426.0 µM for mannose1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • glucose binding Source: InterPro
    • hexokinase activity Source: InterPro
    • N-acetylglucosamine kinase activity Source: CGD

    GO - Biological processi

    • adhesion of symbiont to host Source: CGD
    • cellular glucose homeostasis Source: InterPro
    • cell wall organization Source: UniProtKB-KW
    • development of symbiont in host Source: CGD
    • filamentous growth Source: CGD
    • filamentous growth of a population of unicellular organisms Source: CGD
    • glucosamine catabolic process Source: CGD
    • glycolytic process Source: UniProtKB-KW
    • growth of symbiont in host Source: CGD
    • hyphal growth Source: CGD
    • N-acetylglucosamine catabolic process Source: CGD
    • pathogenesis Source: CGD
    • regulation of phenotypic switching Source: CGD

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processCell wall biogenesis/degradation, Glycolysis, Virulence
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.59 1096

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylglucosamine kinase 11 Publication (EC:2.7.1.591 Publication)
    Short name:
    GlcNAc kinase 1Curated
    Alternative name(s):
    Hexokinase 1Curated
    Gene namesi
    Name:HXK11 Publication
    Synonyms:NAG51 Publication
    Ordered Locus Names:CAALFM_C604580WA
    ORF Names:CaO19.2154, CaO19.9701
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
    Proteomesi
    • UP000000559 Componenti: Chromosome 6

    Organism-specific databases

    CGDiCAL0000186127 HXK1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Greatly retards the growth of cells using GlcNAc as the sole carbon source, increases resistance against farnesol, and attenuates the virulence in a mouse systemic infection model. Leads to derepression of opaque specific gene expression, as well as to constitutive filamentous growth and hyperfilamentation in filamentation-inducing conditions.6 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004317221 – 493N-acetylglucosamine kinase 1Add BLAST493

    Expressioni

    Inductioni

    Expression is induced by N-acetylglucosamine (GlcNAc), by the alpha pheromone, and in filamentation-inducing media.4 Publications

    Interactioni

    Subunit structurei

    Interacts with histone deacetylase SIR2 under filamentation-inducing conditions.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini27 – 490HexokinasePROSITE-ProRule annotationAdd BLAST464

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni79 – 221Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST143
    Regioni222 – 479Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST258

    Sequence similaritiesi

    Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

    Phylogenomic databases

    InParanoidiQ59RG5
    KOiK00844
    OrthoDBiEOG092C2ODC

    Family and domain databases

    InterProiView protein in InterPro
    IPR001312 Hexokinase
    IPR022673 Hexokinase_C
    IPR022672 Hexokinase_N
    PANTHERiPTHR19443 PTHR19443, 1 hit
    PfamiView protein in Pfam
    PF00349 Hexokinase_1, 1 hit
    PF03727 Hexokinase_2, 1 hit
    PROSITEiView protein in PROSITE
    PS51748 HEXOKINASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q59RW5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTETSISGLR GPKSMYFMEI VDVSSQESSV LSSIVESFTS AVSASNLGVY
    60 70 80 90 100
    SDEVLCDIKS SLKENSPITM LPNYNVSPTG DEHGQYLVID LGGSTLRIAV
    110 120 130 140 150
    VDISKPHPNL SRSERITIVV EKSWIIGNDF KRIDGEFFKY IGSKINEILM
    160 170 180 190 200
    GQNVIDVKSV INTGITWSFP LETTDYNRGK IKHVSKGYTV GEDIYDKDLK
    210 220 230 240 250
    MVLEDTLRQE YGLTLDVQSI LNDSLAVYSA GCFIDSKMKL AMVLGTGINM
    260 270 280 290 300
    CCSLKRSSDI HPSKMLADAT LFNCELSLFG QNLCKDFATK YDIIIDKRFA
    310 320 330 340 350
    GLSHHFKTFM EPDPITKTLF QPHELMTSGR YLPELTRLVV VDLIEAGEIF
    360 370 380 390 400
    QNVDHQQMYQ EYGGFSGELI CFVHENDDYD DIHDKLCKAY GWTTVGLSDI
    410 420 430 440 450
    VCLKEVVSCI IKRAAFIVAN AIIAFFKLLG SDELGGDVTI GYVGSVLNYF
    460 470 480 490
    HKYRRLIVEY VNSAEEAKGI KVDLKLIENS SIIGAAIGAA YHK
    Length:493
    Mass (Da):54,823
    Last modified:March 15, 2017 - v2
    Checksum:iF1C4A571B9552FC5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP017628 Genomic DNA Translation: AOW30374.1
    RefSeqiXP_712429.2, XM_707336.2

    Genome annotation databases

    EnsemblFungiiAOW30374; AOW30374; CAALFM_C604580WA
    GeneIDi3645964
    KEGGical:CAALFM_C604580WA

    Similar proteinsi

    Entry informationi

    Entry nameiHXK1_CANAL
    AccessioniPrimary (citable) accession number: Q59RW5
    Secondary accession number(s): A0A1D8PQG2, Q59RG5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
    Last sequence update: March 15, 2017
    Last modified: May 23, 2018
    This is version 78 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health