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Protein

N-acetylglucosamine kinase 1

Gene

HXK1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the N-acetylglucosamine catabolic cascade that phosphorylates N-acetylglucosamine (GlcNAc), and allows the unique ability to utilise GlcNAc as carbon source. Converts GlcNAc to GlcNAc-6-P. Also able to phosphorylate glucose, glucosamine (GlcN), and mannose. Galactose, fructose, N-acetylmannosamine (ManNAc), mannosamine (ManN), galactosamine (GalN), and N-acetylgalactosamine (GalNAc) are not phosphorylated by HXK1. GlcNAc metabolism is closely associated with virulence and morphogenesis, and is involved in the cell wall synthesis. Acts both as a repressor and an activator of genes involved in maintaining cellular homeostasis. Contributes to white-opaque morphological transition and plays a role as a filamentation repressor.7 Publications

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Kineticsi

  1. KM=375.5 µM for N-acetylglucosamine (GlcNAc)1 Publication
  2. KM=482.5 µM for glucose1 Publication
  3. KM=426.0 µM for mannose1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. N-acetylglucosamine kinase activity Source: UniProtKB-EC
  3. phosphotransferase activity, alcohol group as acceptor Source: InterPro

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. glycolytic process Source: UniProtKB-KW
  3. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, Glycolysis, Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.59. 1096.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosamine kinase 11 Publication (EC:2.7.1.591 Publication)
Short name:
GlcNAc kinase 1Curated
Alternative name(s):
Hexokinase 1Curated
Gene namesi
Name:HXK11 Publication
Synonyms:NAG51 Publication
ORF Names:CaO19.2154Imported, CaO19.9701Imported
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0005368. HXK1.
CAL0079099. HXK1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication. Mitochondrion 1 Publication
Note: Localized in cytoplasm and nucleus in a filamentation-inducing medium whereas in 2% GlcNAc, where catabolism is more prominent, a major fraction is seen to be present in cytoplasm. Localizes to mitochondria in non-fermentative carbon sources like ethanol.1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Greatly retards the growth of cells using GlcNAc as the sole carbon source, increases resistance against farnesol, and attenuates the virulence in a mouse systemic infection model. Leads to derepression of opaque specific gene expression, as well as to constitutive filamentous growth and hyperfilamentation in filamentation-inducing conditions.6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493N-acetylglucosamine kinase 1PRO_0000431722Add
BLAST

Expressioni

Inductioni

Expression is induced by N-acetylglucosamine (GlcNAc), by the alpha pheromone, and in filamentation-inducing media.4 Publications

Interactioni

Subunit structurei

Interacts with histone deacetylase SIR2 under filamentation-inducing conditions.1 Publication

Protein-protein interaction databases

STRINGi5476.CAL0005368.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 490464HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 221143Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni222 – 479258Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5026.
InParanoidiQ59RG5.
KOiK00844.
OrthoDBiEOG7NGQMP.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59RW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETSISGLR GPKSMYFMEI VDVSSQESSV LSSIVESFTS AVSASNLGVY
60 70 80 90 100
SDEVLCDIKS SLKENSPITM LPNYNVSPTG DEHGQYLVID LGGSTLRIAV
110 120 130 140 150
VDISKPHPNL SRSERITIVV EKSWIIGNDF KRIDGEFFKY IGSKINEILM
160 170 180 190 200
GQNVIDVKSV INTGITWSFP LETTDYNRGK IKHVSKGYTV GEDIYDKDLK
210 220 230 240 250
MVLEDTLRQE YGLTLDVQSI LNDSLAVYSA GCFIDSKMKL AMVLGTGINM
260 270 280 290 300
CCSLKRSSDI HPSKMLADAT LFNCELSLFG QNLCKDFATK YDIIIDKRFA
310 320 330 340 350
GFSHHFKTFM EPDPITKTLF QPHELMTSGR YLPELTRLVV VDLIEAGEIF
360 370 380 390 400
QNVDHQQMYQ EYGGFSGELM CFVHENDDYD DIHDKLCKAY GWTTVGLSDI
410 420 430 440 450
VCLKEVVSCI IKRAAFIVAN AIIAFFKLLG SDELGGDVTI GYVGSVLNYF
460 470 480 490
HKYRRLIVEY VNNAEEAKGI KVDLKLIENS SIIGAAIGAA YHK
Length:493
Mass (Da):54,902
Last modified:April 25, 2005 - v1
Checksum:iC3D42FD735B13DF0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti302 – 3021F → L in allele: CaO19.9701. Imported
Natural varianti370 – 3701M → I in allele: CaO19.9701. Imported
Natural varianti463 – 4631N → S in allele: CaO19.9701. Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000158 Genomic DNA. Translation: EAK93243.1.
AACQ01000162 Genomic DNA. Translation: EAK93093.1.
RefSeqiXP_712286.1. XM_707193.1.
XP_712429.1. XM_707336.1.

Genome annotation databases

GeneIDi3645964.
3646114.
KEGGical:CaO19.2154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000158 Genomic DNA. Translation: EAK93243.1.
AACQ01000162 Genomic DNA. Translation: EAK93093.1.
RefSeqiXP_712286.1. XM_707193.1.
XP_712429.1. XM_707336.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5476.CAL0005368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3645964.
3646114.
KEGGical:CaO19.2154.

Organism-specific databases

CGDiCAL0005368. HXK1.
CAL0079099. HXK1.

Phylogenomic databases

eggNOGiCOG5026.
InParanoidiQ59RG5.
KOiK00844.
OrthoDBiEOG7NGQMP.

Enzyme and pathway databases

BRENDAi2.7.1.59. 1096.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "The inducible N-acetylglucosamine catabolic pathway gene cluster in Candida albicans: discrete N-acetylglucosamine-inducible factors interact at the promoter of NAG1."
    Kumar M.J., Jamaluddin M.S., Natarajan K., Kaur D., Datta A.
    Proc. Natl. Acad. Sci. U.S.A. 97:14218-14223(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, INDUCTION.
  3. "Identification and characterization of the genes for N-acetylglucosamine kinase and N-acetylglucosamine-phosphate deacetylase in the pathogenic fungus Candida albicans."
    Yamada-Okabe T., Sakamori Y., Mio T., Yamada-Okabe H.
    Eur. J. Biochem. 268:2498-2505(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Attenuation of virulence and changes in morphology in Candida albicans by disruption of the N-acetylglucosamine catabolic pathway."
    Singh P., Ghosh S., Datta A.
    Infect. Immun. 69:7898-7903(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  5. "Use of the porcine intestinal epithelium (PIE)-assay to analyze early stages of colonization by the human fungal pathogen Candida albicans."
    Wendland J., Hellwig D., Walther A., Sickinger S., Shadkchan Y., Martin R., Bauer J., Osherov N., Tretiakov A., Saluz H.P.
    J. Basic Microbiol. 46:513-523(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "The role of nutrient regulation and the Gpa2 protein in the mating pheromone response of C. albicans."
    Bennett R.J., Johnson A.D.
    Mol. Microbiol. 62:100-119(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "N-acetylglucosamine utilization by Saccharomyces cerevisiae based on expression of Candida albicans NAG genes."
    Wendland J., Schaub Y., Walther A.
    Appl. Environ. Microbiol. 75:5840-5845(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Identification of GIG1, a GlcNAc-induced gene in Candida albicans needed for normal sensitivity to the chitin synthase inhibitor nikkomycin Z."
    Gunasekera A., Alvarez F.J., Douglas L.M., Wang H.X., Rosebrock A.P., Konopka J.B.
    Eukaryot. Cell 9:1476-1483(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "N-acetylglucosamine (GlcNAc) induction of hyphal morphogenesis and transcriptional responses in Candida albicans are not dependent on its metabolism."
    Naseem S., Gunasekera A., Araya E., Konopka J.B.
    J. Biol. Chem. 286:28671-28680(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  10. "N-acetylglucosamine kinase, HXK1 is involved in morphogenetic transition and metabolic gene expression in Candida albicans."
    Rao K.H., Ghosh S., Natarajan K., Datta A.
    PLoS ONE 8:E53638-E53638(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, INDUCTION, INTERACTION WITH SIR2, FUNCTION, SUBCELLULAR LOCATION.
  11. "N-acetylglucosamine kinase, HXK1 contributes to white-opaque morphological transition in Candida albicans."
    Rao K.H., Ruhela D., Ghosh S., Abdin M.Z., Datta A.
    Biochem. Biophys. Res. Commun. 445:138-144(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiHXK1_CANAL
AccessioniPrimary (citable) accession number: Q59RW5
Secondary accession number(s): Q59RG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 3, 2015
Last sequence update: April 25, 2005
Last modified: March 31, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.