Reviewed, UniProtKB/Swiss-Prot Q59RK9 (PMIP_CANAL)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	CaO19.1195, CaO19.8786

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 783	750	Mitochondrial intermediate peptidase		PRO_0000338576
Regions
	Compositional bias	27 – 31	5	Poly-Thr
Sites
	Active site	566	1	By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
	Metal binding	569	1	Zinc; catalytic By similarity
	Metal binding	572	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59RK9-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 88739AB0D0DA1928	FASTA	783	89,368
	10 20 30 40 50 60 MKAGIPLSRC TQRLPLLVAR QVSRNITTTT TKFSLAPEYN HIRKVFDSQK YFNHFTKQGS 70 80 90 100 110 120 HATLFSSPQT GLFHNEYLTT PQGLVDFSQQ SLSQAKQLVS EMLSQVNTVD GKLKFIKKLD 130 140 150 160 170 180 QLSDILCRVI DVAEFIRVVH PSVKWINAAQ QTHEMMFEFM NQLNTNVELY SSLRDILNNP 190 200 210 220 230 240 LITEQLTPEE IKVGEYLRQD FERSGIHMDP QTRENFVTIT QEISLLGSQF GNQINGLKSY 250 260 270 280 290 300 WCPVTVTEWE SIEDPQLKKE IKNYQSKYDG IRQPETIQIP LVANIPYTIL TNCSSDTLRK 310 320 330 340 350 360 KVWVALHNSP DEQIETLNRF ISYRALLSKM LNYKSFADYQ LEHKMAKTPE NVITFLSNLQ 370 380 390 400 410 420 KSLIKKGVVE ELSQLSEIKH NGSGSASVND IVNDIKPWDR DYLLARLQQR MQSEVSAGNV 430 440 450 460 470 480 KEYFSVGTVI AGLNELFTRL YDISFVPMAA LKGETWDSHQ VRKIKVVDNA ANKTLGFLYL 490 500 510 520 530 540 DFWSTKVLPS HFTIVCSRRL NTSIGSETIE GMEKLVQLDE DYQLPVVSLV CNFASSGNFS 550 560 570 580 590 600 FGRFAGVENE KPTLLTLDQV DTIFHEMGHA MHSMIGRTEL HNLSGTRCST DFVELPSVLM 610 620 630 640 650 660 ESFSKDPRVI CQIGRHFDTD EKLPESLLGQ AHEHRIMLDA CETFMQSKMA MLDQKLHNEE 670 680 690 700 710 720 MVNLLAKGLY EVDSTKVYHS VEKELKVFAD EWSTWHGKFP HLFSYGAVYY SYLLDRAIAD 730 740 750 760 770 780 KIWQGLFAKD PWNGEAGKKY KESVLKWGGT RDPWECLADA LGNDELKQGD SRAMEIIGQN SNL

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACQ01000161 Genomic DNA. Translation: EAK93122.1. AACQ01000157 Genomic DNA. Translation: EAK93273.1.
RefSeq	XP_712312.1. XP_712456.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	3645934. 3646074.
KEGG	cal:CaO19.1195. cal:CaO19.8786.
Phylogenomic databases
OMA	AMGERYR.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_CANAL

Accession

Primary (citable) accession number: Q59RK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	April 26, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)