Q59R24 (TIM9_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM9

Gene names

Name:	TIM9
ORF Names:	CaJ7.0415, CaO19.13988, CaO19.6696

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space By similarity.
Subunit structure	Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Domain	The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM9 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.
Sequence similarities	Belongs to the small Tim family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 110	110	Mitochondrial import inner membrane translocase subunit TIM9		PRO_0000228041
Regions
	Motif	35 – 59	25	Twin CX3C motif
Amino acid modifications
	Disulfide bond	35 ↔ 59		By similarity
	Disulfide bond	39 ↔ 55		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59R24 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: F257CA919739C00F	FASTA	110	12,957
	10 20 30 40 50 60 MDQLNVKEQQ EFQQIVEQKQ MKDFMNLYSN LVSRCFDDCV NDFTSNSLTS KETSCIAKCS 70 80 90 100 110 EKFLKHSERV GQRFQEQKYV DLSRVTNGNM PLMIIIIYHQ IILTNKLILL

References

[1]	"Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome." Chibana H., Oka N., Nakayama H., Aoyama T., Magee B.B., Magee P.T., Mikami Y. Genetics 170:1525-1537(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006852 Genomic DNA. Translation: BAE44866.1. AACQ01000166 Genomic DNA. Translation: EAK92955.1. AACQ01000167 Genomic DNA. Translation: EAK92929.1.
RefSeq	XP_712123.1. XM_707030.1. XP_712148.1. XM_707055.1. XP_888969.1. XM_883876.1.
3D structure databases
ProteinModelPortal	Q59R24.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0004154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3646243. 3646260. 3704012.
KEGG	cal:CaO19.13988. cal:CaO19.6696. cal:CaO19_6696.
Organism-specific databases
CGD	CAL0004154. orf19.6696.
Phylogenomic databases
eggNOG	NOG330974.
Family and domain databases
Gene3D	1.10.287.810. 1 hit.
InterPro	IPR004217. Tim10/DDP_fam_Znf. [Graphical view]
Pfam	PF02953. zf-Tim10_DDP. 1 hit. [Graphical view]
SUPFAM	SSF144122. SSF144122. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TIM9_CANAL

Accession

Primary (citable) accession number: Q59R24
Secondary accession number(s): Q3MNZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program