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Q59Q46

- IMDH_CANAL

UniProt

Q59Q46 - IMDH_CANAL

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

IMH3

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (22 Feb 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.1 PublicationUniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH. Also inhibited by ADP.1 PublicationUniRule annotation

    Kineticsi

    1. KM=60 µM for Inosine 5'-phosphate1 Publication
    2. KM=3500 µM for NAD+1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi328 – 3281Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi330 – 3301Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei331 – 3311IMPUniRule annotation
    Active sitei333 – 3331Thioimidate intermediateUniRule annotation
    Metal bindingi333 – 3331Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei447 – 4471IMPUniRule annotation
    Metal bindingi506 – 5061Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi507 – 5071Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi508 – 5081Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2783NADUniRule annotation
    Nucleotide bindingi326 – 3283NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: CGD
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular response to drug Source: CGD
    2. cellular response to starvation Source: CGD
    3. filamentous growth Source: CGD
    4. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    5. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
    6. GMP biosynthetic process Source: CGD
    7. induction by symbiont of host defense response Source: CGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:IMH3
    Synonyms:IMD3
    ORF Names:CaO19.18, CaO19.7689
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0069897. IMH3.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi251 – 2511A → T in MPA-resistant strain; decreases the K(M) values for both substrates. Decreases sensitivity to MPA 4-fold, but increases sensitivity to mizoribine monophosphate 40-fold. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 521521Inosine-5'-monophosphate dehydrogenasePRO_0000415684Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ59Q46.
    SMRiQ59Q46. Positions 9-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini119 – 17860CBS 1UniRule annotationAdd
    BLAST
    Domaini182 – 23857CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni366 – 3683IMP bindingUniRule annotation
    Regioni389 – 3902IMP bindingUniRule annotation
    Regioni413 – 4175IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    KOiK00088.
    OrthoDBiEOG793BHK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59Q46-1 [UniParc]FASTAAdd to Basket

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    MVFETSKATS YLKDYPKKDG LSVKELIDST NFGGLTYNDF LILPGLINFP    50
    SSAVSLETKL TKKITLKSPF VSSPMDTVTE ENMAIHMALL GGIGIIHHNC 100
    TSEEQAEMVR KVKKYENGFI NDPVVISPEV TVGEVKKMGE VLGFTSFPVT 150
    ENGKVGGKLV GIITSRDIQF HEDNKSPVSE VMTKDLVVGK KGISLTDGNE 200
    LLRSSKKGKL PIVDAEGNLV SLISRTDLQK NQDYPNASKS FHSKQLLCGA 250
    AIGTIDADRE RLDKLVEAGL DVVVLDSSNG SSVFQLNMIK WIKEKYPELQ 300
    VIAGNVVTRE QAALLIEAGA DALRIGMGSG SICITQEVMA CGRPQGTAVY 350
    GVTEFANKFG VPCIADGGIG NIGHITKALA LGASCVMMGG LLAGTAETPG 400
    DYFYRDGKRL KTYRGMGSID AMQQTNTNAN ASTSRYFSEA DKVLVAQGVS 450
    GSVVDKGSIT KFVPYLYNGL QHSLQDIGIK SIDELRENVD NGEIRFEFRT 500
    ASAQFEGGVH GLHSYEKRLH N 521
    Length:521
    Mass (Da):56,239
    Last modified:February 22, 2012 - v2
    Checksum:i5F1E52611B1E1418
    GO

    Sequence cautioni

    The sequence EAK92581.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAK92603.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY864854 Genomic DNA. Translation: AAW65379.1.
    AACQ01000181 Genomic DNA. Translation: EAK92581.1. Sequence problems.
    AACQ01000180 Genomic DNA. Translation: EAK92603.1. Sequence problems.
    RefSeqiXP_711803.1. XM_706711.1.
    XP_711825.1. XM_706733.1.

    Genome annotation databases

    GeneIDi3646555.
    3646575.
    KEGGical:CaO19.18.
    cal:CaO19.7689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY864854 Genomic DNA. Translation: AAW65379.1 .
    AACQ01000181 Genomic DNA. Translation: EAK92581.1 . Sequence problems.
    AACQ01000180 Genomic DNA. Translation: EAK92603.1 . Sequence problems.
    RefSeqi XP_711803.1. XM_706711.1.
    XP_711825.1. XM_706733.1.

    3D structure databases

    ProteinModelPortali Q59Q46.
    SMRi Q59Q46. Positions 9-519.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3646555.
    3646575.
    KEGGi cal:CaO19.18.
    cal:CaO19.7689.

    Organism-specific databases

    CGDi CAL0069897. IMH3.

    Phylogenomic databases

    KOi K00088.
    OrthoDBi EOG793BHK.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The functional basis of mycophenolic acid resistance in Candida albicans IMP dehydrogenase."
      Koehler G.A., Gong X., Bentink S., Theiss S., Pagani G.M., Agabian N., Hedstrom L.
      J. Biol. Chem. 280:11295-11302(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION, MUTAGENESIS OF ALA-251, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: SC5314 / CAI4 / ATCC MYA-682.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.

    Entry informationi

    Entry nameiIMDH_CANAL
    AccessioniPrimary (citable) accession number: Q59Q46
    Secondary accession number(s): Q5G1M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: February 22, 2012
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3