Q59PR3 (DBP8_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DBP8
EC=3.6.4.13

Gene names

Name:	DBP8
ORF Names:	CaO19.13973, CaO19.6652

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-binding RNA helicase involved in 40S ribosomal subunit biogenesis and is required for the normal formation of 18S rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required for vegetative growth By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus › nucleolus By similarity.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities	Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	ATP-dependent RNA helicase DBP8		PRO_0000232283
Regions
	Domain	32 – 208	177	Helicase ATP-binding
	Domain	247 – 387	141	Helicase C-terminal
	Nucleotide binding	45 – 52	8	ATP By similarity
	Motif	1 – 29	29	Q motif
	Motif	154 – 157	4	DEAD box

Sequences

	Sequence		Length	Mass (Da)
	Q59PR3 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 5A2483E47C9368DB	FASTA	440	48,832
	10 20 30 40 50 60 MSFNDLGVAK WLSESLDAMK IYTPTAIQSA CIPAILKGHD CIGGAKTGSG KTIAFAAPML 70 80 90 100 110 120 TQWSEDPFGI FGLILTPTRE LALQIAEQFA ALGANMNIKV AVVVGGEDFV QQTLALQRKP 130 140 150 160 170 180 HFVIATPGRL ADHILNSGEE TISGLRRVKY LVLDEADRLL SNSFGSDLQR CFDVLPTSDK 190 200 210 220 230 240 RQTLLFTATI TDAVRALKEK PPTPGKPPVF MHEVETVDKV AIPSTLQISY VFVPSYVKEA 250 260 270 280 290 300 YLNSILHLEQ FKDSTAVIFV NRTTTAEVLR RMLRKLDFRV ASLHSEMPQS ERTNSLHRFK 310 320 330 340 350 360 AGAARILIAT DVASRGLDIP TVELVINFDI PADPDDFIHR VGRTARAGRK GDAVSIIGEK 370 380 390 400 410 420 DIDRIQSIEE RINKKMELLE DVNDDNVIKE SLSATSVAKR ESLMEMDKEN FGERKKINRK 430 440 KHGLDKVKPE RVTKPKKSKK

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000165 Genomic DNA. Translation: EAK92999.1. AACQ01000186 Genomic DNA. Translation: EAK92496.1.
RefSeq	XP_711718.1. XM_706626.1. XP_712192.1. XM_707099.1.
3D structure databases
ProteinModelPortal	Q59PR3.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0004058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3646191. 3646692.
KEGG	cal:CaO19.13973. cal:CaO19.6652.
Organism-specific databases
CGD	CAL0004058. DBP8.
Phylogenomic databases
eggNOG	COG0513.
KO	K14778.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DBP8_CANAL

Accession

Primary (citable) accession number: Q59PR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	April 26, 2005
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program