Reviewed, UniProtKB/Swiss-Prot Q59P03 (NCB5R_CANAL)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	CaO19.1801, CaO19.9367

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	NADH-cytochrome b5 reductase 1		PRO_0000330148
Regions
	Transmembrane	18 – 38	21	Potential
	Domain	51 – 154	104	FAD-binding FR-type
	Nucleotide binding	134 – 149	16	FAD By similarity
	Nucleotide binding	160 – 192	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59P03-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: EC4D6DB09B6733B4	FASTA	294	32,472
	10 20 30 40 50 60 MSETTTVPPI ETVSEPNPFI VFATVATIIS AFIGYYFLQQ SKKHTPVLKP DEFQKFPLIE 70 80 90 100 110 120 KIRVSHNSAI YRFGLPKSTD RLGLPIGQHI SIGATIDGKE VVRSYTPIST DDQLGHFDLL 130 140 150 160 170 180 IKTYENGNIS RHVAGKNVGE HIEIRGPKGF FTYTPNMVKS FGMIAGGTGI APMYQIITAI 190 200 210 220 230 240 LKNPEDKTKI HLVYANVTES DILLKEELDN FAARHPDRLK IHYVLNEAPA NWQGSVGFVT 250 260 270 280 290 PEIIDTHLPK ASNDTNLLLC GPPPMVSAMK KAAVELGFQK AKPVSKLGDQ VFVF

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACQ01000198 Genomic DNA. Translation: EAK92221.1. AACQ01000197 Genomic DNA. Translation: EAK92238.1.
RefSeq	XP_711449.1. XP_711466.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3646945. 3646961.
KEGG	cal:CaO19.1801. cal:CaO19.9367.
Organism-specific databases
CGD	CAL0001574. orf19.1801.
Phylogenomic databases
OMA	Q59P03. GNISRHV.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_CANAL

Accession

Primary (citable) accession number: Q59P03

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 26, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)