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Q59NH8 (CDC14_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase CDC14

EC=3.1.3.48
Gene names
Name:CDC14
ORF Names:CaO19.11669, CaO19.4192
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases By similarity. Plays a role in the expression of hydrolase genes such as CHT3 and ENG1 involved in septum degradation after cytokinesis. Also required for ACE2 localization to the daughter nucleus. Required for invasive and hyphal growth. Ref.2 Ref.3 Ref.4

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Nucleusnucleolus. Cytoplasm. Bud neck. Cytoplasmcytoskeletonspindle pole. Cell septum. Note: Does not accumulate in the nucleolus in interphase cells. At the G1-S transition, starts to accumulate both in the nucleolus and the and the DAPI-staining region of the nucleus. At the S-G2 transition, starts to concentrate as faint foci on the nuclear periphery. In early mitosis, the CDC14 nuclear signal diminishes concentrates to the spindle pole body (SPB). During cytokinesis, the localization at the SPBs gets fainter and the protein is preferentially found at the septum region. In yeast forms, in which cell separation takes place after cytokinesis, CDC14 is present at the bud neck; in hyphal forms, in which cell separation is inhibited, no CDC14 signal is detected. Ref.3 Ref.4

Induction

Both during budding and hyphal growth, no detectable levels are observed in G1 cells but, as cells passe through S-phase, begins to accumulate with a peak being reached during mitosis. Ref.3 Ref.5

Post-translational modification

Mainly phosphorylated as the cells are passing through mitosis in yeast form cells. Phosphorylation is delayed in hyphal-induced cells, indicating that the timing of cell-cycle progression occurs with different kinetics in hyphae and in yeast cells. Ref.3

Disruption phenotype

Leads to defective cell separation and impairs hyphal growth. Ref.3

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Meiosis
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   Molecular functionHydrolase
Protein phosphatase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose starvation

Inferred from mutant phenotype Ref.3. Source: CGD

cytokinetic cell separation

Inferred from mutant phenotype Ref.3. Source: CGD

hyphal growth

Inferred from mutant phenotype Ref.3. Source: CGD

invasive growth in response to glucose limitation

Inferred from mutant phenotype Ref.3. Source: CGD

meiotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic nuclear division

Inferred from genetic interaction Ref.2. Source: CGD

regulation of exit from mitosis

Inferred from mutant phenotype Ref.3. Source: CGD

   Cellular_componentcell septum

Inferred from direct assay Ref.3. Source: CGD

cellular bud neck

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleolus

Inferred from genetic interaction Ref.2. Source: CGD

nucleus

Inferred from direct assay Ref.3. Source: CGD

spindle pole body

Inferred from direct assay Ref.3. Source: CGD

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein tyrosine/serine/threonine phosphatase activity

Inferred from genetic interaction Ref.2. Source: CGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Tyrosine-protein phosphatase CDC14
PRO_0000425253

Regions

Compositional bias438 – 4436Poly-Thr

Sites

Active site2271Phosphocysteine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59NH8 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 8B956E794CC0E182

FASTA49455,912
        10         20         30         40         50         60 
MDFGPLNIGH LYRFAVLLHK KLNEDSTQGK GLVIYSSTSP KERANLACLL CCYMILLQNW 

        70         80         90        100        110        120 
APHQVLQPIA QITPPLQAFR DAGYSSADYE ITIQDVVYAM WRAKERGMID LAKFDLDEYE 

       130        140        150        160        170        180 
QYERVDQGDF NVISKDFIAF ASPQQSKRGG LNEPFQKVLE YFVENNVQLV VRLNSHLYDA 

       190        200        210        220        230        240 
KEFTKRNIKH IDMIFDDGTC PTLEYVQKFI GAAECIINKG GKIAVHCKAG LGRTGCLIGA 

       250        260        270        280        290        300 
HLIYTHGFTA NECIAYMRMI RPGMVVGPQQ HWLYLHHDDF RSWRHTMIVD NRPDPLIGNL 

       310        320        330        340        350        360 
FPLCSYEDYK QRLKEAKRKE RLQLQQQLTS PLADSSVINT PIRRRKVSGA LASKIQTVVP 

       370        380        390        400        410        420 
IESPGQPRKY FEDSEDIDEV EMVNNSDDEN TMQDIIQSSP ARYDSVTPKT KDNSDWRVLR 

       430        440        450        460        470        480 
SISTNNVSSQ QSIHIIKTTT TKTVNETLSS PPGTSPTNVL RVSKARSKNR IASGNSQTSR 

       490 
AHSGGVRKLS GKKH 

« Hide

References

« Hide 'large scale' references
[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.
[2]"A single-copy suppressor of the Saccharomyces cerevisae late-mitotic mutants cdc15 and dbf2 is encoded by the Candida albicans CDC14 gene."
Jimenez J., Cid V.J., Nombela C., Sanchez M.
Yeast 18:849-858(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans."
Clemente-Blanco A., Gonzalez-Novo A., Machin F., Caballero-Lima D., Aragon L., Sanchez M., de Aldana C.R., Jimenez J., Correa-Bordes J.
J. Cell Sci. 119:1130-1143(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, PHOSPHORYLATION, DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
[4]"Sep7 is essential to modify septin ring dynamics and inhibit cell separation during Candida albicans hyphal growth."
Gonzalez-Novo A., Correa-Bordes J., Labrador L., Sanchez M., Vazquez de Aldana C.R., Jimenez J.
Mol. Biol. Cell 19:1509-1518(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Transcriptional analysis of the Candida albicans cell cycle."
Cote P., Hogues H., Whiteway M.
Mol. Biol. Cell 20:3363-3373(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000206 Genomic DNA. Translation: EAK92009.1.
AACQ01000205 Genomic DNA. Translation: EAK92033.1.
RefSeqXP_711244.1. XM_706152.1.
XP_711268.1. XM_706176.1.

3D structure databases

ProteinModelPortalQ59NH8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0005886.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3647116.
3647132.
KEGGcal:CaO19.11669.
cal:CaO19.4192.

Organism-specific databases

CGDCAL0073922. CDC14.

Phylogenomic databases

eggNOGCOG2453.
KOK06639.
OrthoDBEOG7JQBXJ.

Family and domain databases

Gene3D3.90.190.10. 2 hits.
InterProIPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR23339:SF27. PTHR23339:SF27. 1 hit.
PfamPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view]
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 2 hits.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDC14_CANAL
AccessionPrimary (citable) accession number: Q59NH8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: April 26, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names