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Protein

Tyrosine-protein phosphatase CDC14

Gene

CDC14

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases (By similarity). Plays a role in the expression of hydrolase genes such as CHT3 and ENG1 involved in septum degradation after cytokinesis. Also required for ACE2 localization to the daughter nucleus. Required for invasive and hyphal growth.By similarity3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell separation after cytokinesis Source: CGD
  • cellular response to glucose starvation Source: CGD
  • hyphal growth Source: CGD
  • invasive growth in response to glucose limitation Source: CGD
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic cell cycle Source: CGD
  • mitotic cell cycle arrest Source: GO_Central
  • mitotic spindle midzone assembly Source: GO_Central
  • regulation of exit from mitosis Source: CGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase CDC14 (EC:3.1.3.48)
Gene namesi
Name:CDC14
Ordered Locus Names:CAALFM_C600670WA
ORF Names:CaO19.11669, CaO19.4192
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 6

Organism-specific databases

CGDiCAL0000180943. CDC14.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to defective cell separation and impairs hyphal growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004252531 – 542Tyrosine-protein phosphatase CDC14Add BLAST542

Post-translational modificationi

Mainly phosphorylated as the cells are passing through mitosis in yeast form cells. Phosphorylation is delayed in hyphal-induced cells, indicating that the timing of cell-cycle progression occurs with different kinetics in hyphae and in yeast cells.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ59NH8.

Expressioni

Inductioni

Both during budding and hyphal growth, no detectable levels are observed in G1 cells but, as cells passe through S-phase, begins to accumulate with a peak being reached during mitosis.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ59NH8.
SMRiQ59NH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi486 – 491Poly-Thr6

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ59NH8.
KOiK06639.
OrthoDBiEOG092C2FOY.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiView protein in InterPro
IPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
PANTHERiPTHR23339:SF84. PTHR23339:SF84. 1 hit.
PfamiView protein in Pfam
PF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
SMARTiView protein in SMART
SM00195. DSPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiView protein in PROSITE
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59NH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSSSVHVPL IEFLKNRIYL GAYDHHKRDT EDLAYFTVED ALPYNAFYMD
60 70 80 90 100
FGPLNIGHLY RFAVLLHKKL NEDSTQGKGL VIYSSTSPKE RANLACLLCC
110 120 130 140 150
YMILLQNWAP HQVLQPIAQI TPPLQAFRDA GYSSADYEIT IQDVVYAMWR
160 170 180 190 200
AKERGMIDLA KFDLDEYEQY ERVDQGDFNV ISKDFIAFAS PQQSKRGGLN
210 220 230 240 250
EPFQKVLEYF VENNVQLVVR LNSHLYDAKE FTKRNIKHID MIFDDGTCPT
260 270 280 290 300
LEYVQKFIGA AECIINKGGK IAVHCKAGLG RTGCLIGAHL IYTHGFTANE
310 320 330 340 350
CIAYMRMIRP GMVVGPQQHW LYLHHDDFRS WRHTMIVDNR PDPLIGNLFP
360 370 380 390 400
LCSYEDYKQR LKEAKRKERL QLQQQLTSPL ADSSVINTPI RRRKVSGALA
410 420 430 440 450
SKIQTVVPIE SPGQPRKYFE DSEDIDEVEM VNNSDDENTM QDIIQSSPAR
460 470 480 490 500
YDSVTPKTKD NSDWRVLRSI STNNVSSQQS IHIIKTTTTK TVNETLSSPP
510 520 530 540
GTSPTNVLRV SKARSKNRIA SGNSQTSRAH SGGVRKLSGK KH
Length:542
Mass (Da):61,582
Last modified:March 15, 2017 - v2
Checksum:i34E207CFCA770FE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017628 Genomic DNA. Translation: AOW30013.1.
RefSeqiXP_019330990.1. XM_019475445.1.

Genome annotation databases

GeneIDi3647132.
KEGGical:CAALFM_C600670WA.

Similar proteinsi

Entry informationi

Entry nameiCDC14_CANAL
AccessioniPrimary (citable) accession number: Q59NH8
Secondary accession number(s): A0A1D8PPE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: March 15, 2017
Last modified: September 27, 2017
This is version 84 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families