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Q59NH8

- CDC14_CANAL

UniProt

Q59NH8 - CDC14_CANAL

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Protein

Tyrosine-protein phosphatase CDC14

Gene

CDC14

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases (By similarity). Plays a role in the expression of hydrolase genes such as CHT3 and ENG1 involved in septum degradation after cytokinesis. Also required for ACE2 localization to the daughter nucleus. Required for invasive and hyphal growth.By similarity3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei227 – 2271Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. protein tyrosine/serine/threonine phosphatase activity Source: CGD
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular response to glucose starvation Source: CGD
  2. cytokinetic cell separation Source: CGD
  3. hyphal growth Source: CGD
  4. invasive growth in response to glucose limitation Source: CGD
  5. meiotic nuclear division Source: UniProtKB-KW
  6. mitotic nuclear division Source: CGD
  7. regulation of exit from mitosis Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase CDC14 (EC:3.1.3.48)
Gene namesi
Name:CDC14
ORF Names:CaO19.11669, CaO19.4192
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0073922. CDC14.

Subcellular locationi

Nucleusnucleolus. Cytoplasm. Bud neck. Cytoplasmcytoskeletonspindle pole. Cell septum
Note: Does not accumulate in the nucleolus in interphase cells. At the G1-S transition, starts to accumulate both in the nucleolus and the and the DAPI-staining region of the nucleus. At the S-G2 transition, starts to concentrate as faint foci on the nuclear periphery. In early mitosis, the CDC14 nuclear signal diminishes concentrates to the spindle pole body (SPB). During cytokinesis, the localization at the SPBs gets fainter and the protein is preferentially found at the septum region. In yeast forms, in which cell separation takes place after cytokinesis, CDC14 is present at the bud neck; in hyphal forms, in which cell separation is inhibited, no CDC14 signal is detected.

GO - Cellular componenti

  1. cell septum Source: CGD
  2. cytoplasm Source: UniProtKB-KW
  3. nucleolus Source: CGD
  4. nucleus Source: CGD
  5. spindle pole body Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to defective cell separation and impairs hyphal growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Tyrosine-protein phosphatase CDC14PRO_0000425253Add
BLAST

Post-translational modificationi

Mainly phosphorylated as the cells are passing through mitosis in yeast form cells. Phosphorylation is delayed in hyphal-induced cells, indicating that the timing of cell-cycle progression occurs with different kinetics in hyphae and in yeast cells.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Both during budding and hyphal growth, no detectable levels are observed in G1 cells but, as cells passe through S-phase, begins to accumulate with a peak being reached during mitosis.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0005886.

Structurei

3D structure databases

ProteinModelPortaliQ59NH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi438 – 4436Poly-Thr

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
InParanoidiQ59NH8.
KOiK06639.
OrthoDBiEOG7JQBXJ.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR23339:SF27. PTHR23339:SF27. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59NH8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFGPLNIGH LYRFAVLLHK KLNEDSTQGK GLVIYSSTSP KERANLACLL
60 70 80 90 100
CCYMILLQNW APHQVLQPIA QITPPLQAFR DAGYSSADYE ITIQDVVYAM
110 120 130 140 150
WRAKERGMID LAKFDLDEYE QYERVDQGDF NVISKDFIAF ASPQQSKRGG
160 170 180 190 200
LNEPFQKVLE YFVENNVQLV VRLNSHLYDA KEFTKRNIKH IDMIFDDGTC
210 220 230 240 250
PTLEYVQKFI GAAECIINKG GKIAVHCKAG LGRTGCLIGA HLIYTHGFTA
260 270 280 290 300
NECIAYMRMI RPGMVVGPQQ HWLYLHHDDF RSWRHTMIVD NRPDPLIGNL
310 320 330 340 350
FPLCSYEDYK QRLKEAKRKE RLQLQQQLTS PLADSSVINT PIRRRKVSGA
360 370 380 390 400
LASKIQTVVP IESPGQPRKY FEDSEDIDEV EMVNNSDDEN TMQDIIQSSP
410 420 430 440 450
ARYDSVTPKT KDNSDWRVLR SISTNNVSSQ QSIHIIKTTT TKTVNETLSS
460 470 480 490
PPGTSPTNVL RVSKARSKNR IASGNSQTSR AHSGGVRKLS GKKH
Length:494
Mass (Da):55,912
Last modified:April 26, 2005 - v1
Checksum:i8B956E794CC0E182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000206 Genomic DNA. Translation: EAK92009.1.
AACQ01000205 Genomic DNA. Translation: EAK92033.1.
RefSeqiXP_711244.1. XM_706152.1.
XP_711268.1. XM_706176.1.

Genome annotation databases

GeneIDi3647116.
3647132.
KEGGical:CaO19.11669.
cal:CaO19.4192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000206 Genomic DNA. Translation: EAK92009.1 .
AACQ01000205 Genomic DNA. Translation: EAK92033.1 .
RefSeqi XP_711244.1. XM_706152.1.
XP_711268.1. XM_706176.1.

3D structure databases

ProteinModelPortali Q59NH8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0005886.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3647116.
3647132.
KEGGi cal:CaO19.11669.
cal:CaO19.4192.

Organism-specific databases

CGDi CAL0073922. CDC14.

Phylogenomic databases

eggNOGi COG2453.
InParanoidi Q59NH8.
KOi K06639.
OrthoDBi EOG7JQBXJ.

Family and domain databases

Gene3Di 3.90.190.10. 2 hits.
InterProi IPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR23339:SF27. PTHR23339:SF27. 1 hit.
Pfami PF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "A single-copy suppressor of the Saccharomyces cerevisae late-mitotic mutants cdc15 and dbf2 is encoded by the Candida albicans CDC14 gene."
    Jimenez J., Cid V.J., Nombela C., Sanchez M.
    Yeast 18:849-858(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans."
    Clemente-Blanco A., Gonzalez-Novo A., Machin F., Caballero-Lima D., Aragon L., Sanchez M., de Aldana C.R., Jimenez J., Correa-Bordes J.
    J. Cell Sci. 119:1130-1143(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, PHOSPHORYLATION, DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
  4. "Sep7 is essential to modify septin ring dynamics and inhibit cell separation during Candida albicans hyphal growth."
    Gonzalez-Novo A., Correa-Bordes J., Labrador L., Sanchez M., Vazquez de Aldana C.R., Jimenez J.
    Mol. Biol. Cell 19:1509-1518(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Transcriptional analysis of the Candida albicans cell cycle."
    Cote P., Hogues H., Whiteway M.
    Mol. Biol. Cell 20:3363-3373(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCDC14_CANAL
AccessioniPrimary (citable) accession number: Q59NH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3