Q59NG5 (MUS81_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endonuclease MUS81
EC=3.1.22.-

Gene names

Name:	MUS81
ORF Names:	CaO19.11682, CaO19.4206

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI) By similarity.
Cofactor	Magnesium By similarity.
Subunit structure	Interacts with EME1 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the XPF family. Contains 1 ERCC4 domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Meiosis
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Crossover junction endonuclease MUS81		PRO_0000223641
Regions
	Domain	324 – 421	98	ERCC4

Sequences

	Sequence		Length	Mass (Da)
	Q59NG5 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 11B248C3953A29A2	FASTA	614	70,663
	10 20 30 40 50 60 MSVVNDDFKP LLIQWVEEEA INATKRGSKM VDLYNKILFQ LRGYELPICD LKTLKSIKYV 70 80 90 100 110 120 GDKTANQLRK KIAKHCKENE LTLPRGFMDV AEKHRLDLEE SNLTSSTSTI KTTNKRAKTS 130 140 150 160 170 180 SKSKPKYVPK HRSGGFAILI ALYLYDKDRN GMTKERIIAG ATPYCDRSFS NNAASKEFYS 190 200 210 220 230 240 AWSSAKTLEN HNLISTTGRS PKIYFLTDEG VSLAQQLKTA IGLSSPPEGS KKNDRNMIME 250 260 270 280 290 300 QSFDNGIRLD TSFELSSPAG RAMLSSSPIR RISNPVHSNR AIQKMLEKEL RNGETPPSSQ 310 320 330 340 350 360 HDAGNRIYDG IKYEVWRKED YEVIVYMDNR EIRSRADRDH FQTRLQTLGV KCEVKPLSSG 370 380 390 400 410 420 DVLWVGRNTS TGTEAVLNYL CERKRLDDLC DSIKDGRFQE QKNRMKKTGI KHCYYLVEDM 430 440 450 460 470 480 VSYQDKVYDL MDSIQSSLTQ TMTTARLYLR RFKDIDETTA FIASNTKVIE NLKSNLIVIK 490 500 510 520 530 540 PQDIKNQQDY LNILLKFRNK FEKTQPHQDN PDYECVQLFS RFQDMLGKTN QMTVKEMFIL 550 560 570 580 590 600 MLMTIRGVSL EKAVVIQNRF PTPKSLLEYY HTEHATTDTN IKRDLMMNEF KDQIGNKKIG 610 KALSEKIYNV WGKP

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000206 Genomic DNA. Translation: EAK92023.1. AACQ01000205 Genomic DNA. Translation: EAK92046.1.
RefSeq	XP_711258.1. XM_706166.1. XP_711281.1. XM_706189.1.
3D structure databases
ProteinModelPortal	Q59NG5.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0000062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647121. 3647146.
KEGG	cal:CaO19.11682. cal:CaO19.4206.
Organism-specific databases
CGD	CAL0000062. orf19.4206.
Phylogenomic databases
eggNOG	COG1948.
KO	K08991.
Family and domain databases
Gene3D	3.40.50.10130. 1 hit.
InterPro	IPR020819. DNA_repair_nuc_XPF/helicase. IPR006166. ERCC4_domain. IPR011335. Restrct_endonuc-II-like. [Graphical view]
Pfam	PF02732. ERCC4. 1 hit. [Graphical view]
SMART	SM00891. ERCC4. 1 hit. [Graphical view]
SUPFAM	SSF52980. Restrict_endonuc_II-like_core. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MUS81_CANAL

Accession

Primary (citable) accession number: Q59NG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program