Q59LR2 (ATR_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase MEC1
EC=2.7.11.1

Alternative name(s):
ATR homolog
DNA-damage checkpoint kinase MEC1
Mitosis entry checkpoint protein 1

Gene names

Name:	MEC1
ORF Names:	CaO19.1283, CaO19.8870

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	2325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Recruited to DNA lesions in order to initiate the DNA repair by homologous recombination. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, also involved in the regulation of DNA damage response mechanism. Required for cell growth and meiotic recombination By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity. Note: Localizes to nuclear DNA repair foci in response to DNA double strand breaks By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Meiosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chromatin regulator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW chromosome organization Inferred from mutant phenotype PubMed 21511048. Source: CGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2325	2325	Serine/threonine-protein kinase MEC1		PRO_0000227709
Regions
	Domain	1363 – 1886	524	FAT
	Domain	2019 – 2325	307	PI3K/PI4K
	Domain	2293 – 2325	33	FATC
Natural variations
	Natural variant	803	1	I → V in allele CaO19.8870.
	Natural variant	915	1	N → D in allele CaO19.8870.
	Natural variant	995	1	E → D in allele CaO19.8870.
	Natural variant	1108	1	S → Q in allele CaO19.8870.
	Natural variant	1170	1	T → I in allele CaO19.8870.
	Natural variant	1252	1	Y → C in allele CaO19.8870.
	Natural variant	1523	1	T → I in allele CaO19.8870.
	Natural variant	1601	1	Q → K in allele CaO19.8870.
	Natural variant	1899	1	L → F in allele CaO19.8870.

Sequences

	Sequence		Length	Mass (Da)
	Q59LR2 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: B82E8A4C049EA6B5	FASTA	2,325	266,517
	10 20 30 40 50 60 MTSNQSISTT ELLQFLTDIE TNIDDHETDF RKLLLYLLRF TNEKLIVIAQ EENKTPTELQ 70 80 90 100 110 120 LLSKLIDTIE LVLSKKTPLL STLLTIEDVN IIHTTGSGSL VYEVPLHEWC ISFALSHIPN 130 140 150 160 170 180 FVSHTAGLNQ LKRLVFLIVN LVSTQLHSFK VIKSTRIHLL KTLDDNLNFC LQNLLSANTF 190 200 210 220 230 240 LFKSKLTTAV NLFSIVHDYD ISQKLSLNLN NYQLKFESCS RKIWFILNEI SLVSELDNLN 250 260 270 280 290 300 LLDCLKSVFI LDQSSALVLN VSVGWNQIGF LLSCIVEYLQ QDFRTLDSNT NNFEFVNLNR 310 320 330 340 350 360 SISLSLLNVY IVCFDKDLLE NFMSFSNIKG ILSKLIYDDS IPSVIRKTLN IVQYTYQLMS 370 380 390 400 410 420 NPDGDDIKLY STSVYNDYVW TPFVDSELES LRARLLDLQG NHEDSRKEEL LSFSIDETHK 430 440 450 460 470 480 LAKSTNSLNY TDEKAWIRTV KKLIGIDKNV LEDETTLYTL VTALSHYPCI LKGDYDYTIN 490 500 510 520 530 540 ECTKCGFGPL TKNNYSSIDP NRFPLNYSTE ATTLQDIIQQ FLIPKLETQQ DPLLCCNTLL 550 560 570 580 590 600 LIFNFYASFS PMADMQDHNI LDFLLRLLAT NDNRDVRMLV ARILPLYLIQ SKDDKLLDET 610 620 630 640 650 660 FKYIFQKVTS IDFSSQHRLH FGESTIRAVV ELATVSTGER LCAIYFKLVD WLGEQNEQHS 670 680 690 700 710 720 NYVYCGILNL ASAKSLPPHK LLSPYLPSVA EIIIKKPQVF ERIIKVSMVT KNYFLNRTKE 730 740 750 760 770 780 YTVPRILEYY KDPTLLTQIA NAAGLEVGKL LANCLPRILA TYLTKESVNE RYIMKVLSSV 790 800 810 820 830 840 CPDYKMIHTE ELFTRIGDIT WYILLEIQMD EFGNIRNLAN ITRALECVCK NVSLRKNGSE 850 860 870 880 890 900 LTKNNSINDL IEDQVLLLVQ KFSDVTHSSR GAKPYLELKN SFYAIEFLIK GHIDAITSAL 910 920 930 940 950 960 GQLSTCLQAT LEEPNFHVLS LRCWNELIKK VPPSHLISLI DIIISIILQK FESFGSEAKS 970 980 990 1000 1010 1020 IAIEILRKIY EEIKDKYNRY SLYFLSLPFL SYMEEYQMVK EFRNMKSPSR AMIFSEFTRR 1030 1040 1050 1060 1070 1080 LQTSNMYVVK HALFDLSNYF EKYQINCQKD LFKDPGLTPA ITSLVRTILD TAAKFKNKDT 1090 1100 1110 1120 1130 1140 TVSTACAKAL AIIGALDSNK FQFKTVKSLI IISSDFEDIE ENSTFLVDLI ENHLLKIFWA 1150 1160 1170 1180 1190 1200 SNDPHKQLFA AYVMQSFLAV MGLDERVLNT KDNRVWNKFT DVAKSTLTPF LKSKYAAPKP 1210 1220 1230 1240 1250 1260 KLDNLKFPFF KLGMKYETWL VDVTLFLLKR ASIDNGKGNQ KAKTRKLIFQ SYAVLIQREH 1270 1280 1290 1300 1310 1320 DIPLCEHLLR YVALSHVINE GVPEDLHKEF LHILKMDSKS TSPDRAEQLK LCYQAIFSVL 1330 1340 1350 1360 1370 1380 DYFNQWVSNM RVVTSNSGSE LTSSDIRHKM DAVAKFSSFP QDLLTTRSAE CDAYERTIMY 1390 1400 1410 1420 1430 1440 LENCYRDSQS EKSFKLSNLN GAATLQDMYA HIDDYDALNG TLKMFSTNNL NEKLTTFQYS 1450 1460 1470 1480 1490 1500 DSWSLAHESF EALGSTKNSV SNNTKLLQSL NEHGLYNEVL STLSARTDSN DLKSIPLDWS 1510 1520 1530 1540 1550 1560 LMGLHAAVYK GDSKQLEKWL QVTNSIGKPH DMETMINYEL AKALSFLFQS RIDMFKGSMD 1570 1580 1590 1600 1610 1620 KLYNIIGCSL VPSVSSNFTR NITLMNQLHA IYDVSLIVLS QDSEDTLDLR IGNVDQDFDT 1630 1640 1650 1660 1670 1680 QRNILTLHNV ANTVMKNPAM ISKNLLRESS LARKYNRLDI STRSIVQAMS LEDDQANIEF 1690 1700 1710 1720 1730 1740 AELQWAQGKQ SEAIKCLFDI LKDNKFHDDK SKAKVQLQYA NWLDESNHLS AHQIITEYNK 1750 1760 1770 1780 1790 1800 AFHLNMVDEK CNFDIGKYYN KLMESSNDES GEYEHLTVRN YIRAVSVGTT YIFEALPKVL 1810 1820 1830 1840 1850 1860 TIWLDFADKS NKSNAAENRL KQIIDDLYNA IANVPNYSWY TVLTQILSRI VHEHEPSFKV 1870 1880 1890 1900 1910 1920 LKRIVQNVTL EYPKHCVWYI FSHARSSDKV RKRRVRELLN QVCTQDGNDT LPKSTMAAGN 1930 1940 1950 1960 1970 1980 LFAKLIKIAE LKIPKTNRKR QMSLLQDFEV DLSEPIDDLV IPIQSNLQIQ IPSHLNSKHK 1990 2000 2010 2020 2030 2040 GFSRSSSISF DGFDDNVNIF FSLQMPRQLT VRGSDGNAYR LMVKSDDTRK DAKVVEFTTM 2050 2060 2070 2080 2090 2100 VNRILSTSTE ARKRGLQIAN YSVVPLSDHF GIIEFVMNVQ TMKGVISEQR KRQGIPINER 2110 2120 2130 2140 2150 2160 KVFMHIDSLQ KAKKKDSKQL DKLVAGFRAI MDRCPPVLHT WFVEQFSDPS AWYMARNAFT 2170 2180 2190 2200 2210 2220 RSSAVMSMVG YIMGLGDRHC ENILIFKNTG AVLHIDFDCL FEKGTTLPTP EIVPFRLTQN 2230 2240 2250 2260 2270 2280 MVDAMGITGV DGIYRITCEV TGTLLRENEQ ILMNILETLI YDPLIDWRNH NPREDLSKVR 2290 2300 2310 2320 KKIRGLINED EGLPMNIHGQ VDVLIQEATS LERLSQMYAG WAAYM

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000249 Genomic DNA. Translation: EAK91416.1. AACQ01000250 Genomic DNA. Translation: EAK91407.1.
RefSeq	XP_710655.1. XM_705563.1. XP_710664.1. XM_705572.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0003061.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647733. 3647742.
KEGG	cal:CaO19.1283. cal:CaO19.8870.
Organism-specific databases
CGD	CAL0003061. MEC1.
Phylogenomic databases
eggNOG	COG5032.
KO	K02543.
Family and domain databases
Gene3D	1.10.1070.11. 2 hits.
InterPro	IPR016024. ARM-type_fold. IPR003152. FATC. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR003151. PIK-rel_kinase_FAT. IPR014009. PIK_FAT. IPR012993. UME. [Graphical view]
Pfam	PF02259. FAT. 1 hit. PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. PF08064. UME. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. SM00802. UME. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATR_CANAL

Accession

Primary (citable) accession number: Q59LR2
Secondary accession number(s): Q59LQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program