Q59LF2 (ALG2_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2
EC=2.4.1.132
EC=2.4.1.257

Alternative name(s):
Asparagine-linked glycosylation protein 2
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Gene names

Name:	ALG2
ORF Names:	CaO19.1221, CaO19.8808

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate By similarity.
Catalytic activity	GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol. GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Alpha-1,3/1,6-mannosyltransferase ALG2		PRO_0000080263
Regions
	Transmembrane	80 – 100	21	Helical; Potential
Amino acid modifications
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q59LF2 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 21F8239880384A72	FASTA	428	48,767
	10 20 30 40 50 60 MSKRQENKKI AFIHPDLGIG GAERLVVDAA VGLQDFGHDI IIYTSHCDLT HCFEEVSSGQ 70 80 90 100 110 120 LKVSVHGDSL PTNLFGKLHI FFAILRQFYL VCWLIFTGTI KNYDYFIVDQ LSFCIPLLKM 130 140 150 160 170 180 FCNSNCQVLF YCHFPDQLLV RRTSFLKKLY RVPFDAIEEY TTGSSDQIVV NSNFTKQIFH 190 200 210 220 230 240 DTFKKLNHID PQVVYPCVDT ETIVDTNTSS NSEVSKFFKD SPFFLSINRF ERSKNIELAI 250 260 270 280 290 300 KSFARMNKLM VTNKKPRLVI AGGYDSRVAE NVEYLAELST LCDELNLINF TIRGKLIMMP 310 320 330 340 350 360 PSVDVLFLPS ISTQLKNSLI QQTELLLYTP PREHFGIVPL EAMLAKTPVL AINFGGPLET 370 380 390 400 410 420 VVNYNGNNLD EATGYTETGD FTKWSKIIMK HYNLDESTKI KLGENGRNRV INKFSRKKLA QSLDNILN

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000257 Genomic DNA. Translation: EAK91318.1. AACQ01000256 Genomic DNA. Translation: EAK91329.1.
RefSeq	XP_710571.1. XM_705479.1. XP_710581.1. XM_705489.1.
3D structure databases
ProteinModelPortal	Q59LF2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q59LF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647825. 3647832.
KEGG	cal:CaO19.1221. cal:CaO19.8808.
Organism-specific databases
CGD	CAL0002829. ALG2.
Phylogenomic databases
PhylomeDB	Q59LF2.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03843.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG2_CANAL

Accession

Primary (citable) accession number: Q59LF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program