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Protein

Serine/threonine-protein phosphatase SIT4

Gene

SIT4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine protein phosphatase which is involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1 (By similarity). Plays a role during hyphal growth through the regulation of cell wall biogenesis, osmosensing and protein translation. Involved in virulence in a mouse systemic infection model.By similarity2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Manganese 1By similarity1
Metal bindingi55Manganese 1By similarity1
Metal bindingi85Manganese 1By similarity1
Metal bindingi85Manganese 2By similarity1
Metal bindingi117Manganese 2By similarity1
Active sitei118Proton donorBy similarity1
Metal bindingi167Manganese 2By similarity1
Metal bindingi241Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: CGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to neutral pH Source: CGD
  • filamentous growth Source: CGD
  • filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  • filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  • filamentous growth of a population of unicellular organisms in response to heat Source: CGD
  • filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
  • filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  • pathogenesis Source: CGD
  • positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  • positive regulation of filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
  • positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  • protein phosphorylation Source: CGD
  • TOR signaling Source: CGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis, Virulence
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase SIT4 (EC:3.1.3.16)
Gene namesi
Name:SIT4
Ordered Locus Names:CAALFM_C104380WA
ORF Names:CaO19.12667, CaO19.5200
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 1

Organism-specific databases

CGDiCAL0000187173 SIT4

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes growth retardation and leads to significant reduction in virulence.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221071 – 314Serine/threonine-protein phosphatase SIT4Add BLAST314

Proteomic databases

PRIDEiQ59KY8

Interactioni

Subunit structurei

Interacts with MDS3.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ59KY8
SMRiQ59KY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000172696
InParanoidiQ59KY8
KOiK15427
OMAiKYCCRVF
OrthoDBiEOG092C2R40

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q59KY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGERGPDQWL EQIKNCISLS ESDMKQLCEL VKELLMEESN IQPVQSPVTV
60 70 80 90 100
CGDIHGQFHD LLELFRTAGG LPSDDNQTNF IFLGDYVDRG YFSLETFTLL
110 120 130 140 150
MVLKVKYPHR ITLVRGNHES RQITQVYGFY EECLTKYGST TVWKYCCQVF
160 170 180 190 200
DFLTLAAIID GKILCVHGGL SPEIRMLDQI RVLSRAQEVP HEGGFCDLVW
210 220 230 240 250
SDPDNIDTWA VSPRGAGWLF GSKVSREFNH INNLQLIARA HQLVMEGFRY
260 270 280 290 300
HFKEKDVVTV WSAPNYCYRC GNVASVMQVD EDLEPNFKIF SAVQDGDLSV
310
KNNANKQQRS DYFL
Length:314
Mass (Da):35,899
Last modified:April 26, 2005 - v1
Checksum:i9D9577F956F7580F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017623 Genomic DNA Translation: AOW26112.1
RefSeqiXP_710408.1, XM_705316.2

Genome annotation databases

EnsemblFungiiAOW26112; AOW26112; CAALFM_C104380WA
GeneIDi3647993
KEGGical:CAALFM_C104380WA

Similar proteinsi

Entry informationi

Entry nameiSIT4_CANAL
AccessioniPrimary (citable) accession number: Q59KY8
Secondary accession number(s): A0A1D8PD98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: April 26, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health