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Q59KY8 (SIT4_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase SIT4

EC=3.1.3.16
Gene names
Name:SIT4
ORF Names:CaO19.12667, CaO19.5200
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine protein phosphatase which is involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1 By similarity. Plays a role during hyphal growth through the regulation of cell wall biogenesis, osmosensing and protein translation. Involved in virulence in a mouse systemic infection model. Ref.2 Ref.3

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

Interacts with MDS3. Ref.3

Subcellular location

Cytoplasm By similarity.

Disruption phenotype

Causes growth retardation and leads to significant reduction in virulence. Ref.2

Sequence similarities

Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Virulence
   Cellular componentCytoplasm
   LigandIron
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processTOR signaling

Inferred from genetic interaction Ref.3. Source: CGD

cellular response to DNA damage stimulus

Inferred from mutant phenotype PubMed 21890819. Source: CGD

cellular response to drug

Inferred from mutant phenotype PubMed 22606354. Source: CGD

cellular response to neutral pH

Inferred from mutant phenotype Ref.2. Source: CGD

filamentous growth

Inferred from mutant phenotype Ref.2Ref.3. Source: CGD

filamentous growth of a population of unicellular organisms in response to biotic stimulus

Inferred from mutant phenotype Ref.2. Source: CGD

filamentous growth of a population of unicellular organisms in response to chemical stimulus

Inferred from mutant phenotype Ref.3. Source: CGD

filamentous growth of a population of unicellular organisms in response to heat

Inferred from mutant phenotype Ref.3. Source: CGD

filamentous growth of a population of unicellular organisms in response to neutral pH

Inferred from mutant phenotype Ref.2. Source: CGD

filamentous growth of a population of unicellular organisms in response to starvation

Inferred from mutant phenotype Ref.2. Source: CGD

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from mutant phenotype Ref.2. Source: CGD

positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus

Inferred from mutant phenotype Ref.3. Source: CGD

positive regulation of filamentous growth of a population of unicellular organisms in response to neutral pH

Inferred from mutant phenotype Ref.2. Source: CGD

positive regulation of filamentous growth of a population of unicellular organisms in response to starvation

Inferred from mutant phenotype Ref.2. Source: CGD

protein dephosphorylation

Inferred from mutant phenotype PubMed 21890819. Source: CGD

protein phosphorylation

Inferred from genetic interaction Ref.2. Source: CGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine phosphatase activity

Inferred from genetic interaction Ref.2. Source: CGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Serine/threonine-protein phosphatase SIT4
PRO_0000422107

Sites

Active site1181Proton donor By similarity
Metal binding531Iron By similarity
Metal binding551Iron By similarity
Metal binding851Iron By similarity
Metal binding851Manganese By similarity
Metal binding1171Manganese By similarity
Metal binding1671Manganese By similarity
Metal binding2411Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59KY8 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 9D9577F956F7580F

FASTA31435,899
        10         20         30         40         50         60 
MGERGPDQWL EQIKNCISLS ESDMKQLCEL VKELLMEESN IQPVQSPVTV CGDIHGQFHD 

        70         80         90        100        110        120 
LLELFRTAGG LPSDDNQTNF IFLGDYVDRG YFSLETFTLL MVLKVKYPHR ITLVRGNHES 

       130        140        150        160        170        180 
RQITQVYGFY EECLTKYGST TVWKYCCQVF DFLTLAAIID GKILCVHGGL SPEIRMLDQI 

       190        200        210        220        230        240 
RVLSRAQEVP HEGGFCDLVW SDPDNIDTWA VSPRGAGWLF GSKVSREFNH INNLQLIARA 

       250        260        270        280        290        300 
HQLVMEGFRY HFKEKDVVTV WSAPNYCYRC GNVASVMQVD EDLEPNFKIF SAVQDGDLSV 

       310 
KNNANKQQRS DYFL 

« Hide

References

« Hide 'large scale' references
[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.
[2]"The serine/threonine protein phosphatase SIT4 modulates yeast-to-hypha morphogenesis and virulence in Candida albicans."
Lee C.M., Nantel A., Jiang L., Whiteway M., Shen S.H.
Mol. Microbiol. 51:691-709(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[3]"Mds3 regulates morphogenesis in Candida albicans through the TOR pathway."
Zacchi L.F., Gomez-Raja J., Davis D.A.
Mol. Cell. Biol. 30:3695-3710(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MDS3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000273 Genomic DNA. Translation: EAK91146.1.
AACQ01000272 Genomic DNA. Translation: EAK91157.1.
RefSeqXP_710408.1. XM_705316.1.
XP_710419.1. XM_705327.1.

3D structure databases

ProteinModelPortalQ59KY8.
SMRQ59KY8. Positions 7-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0003480.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3647982.
3647993.
KEGGcal:CaO19.12667.
cal:CaO19.5200.

Organism-specific databases

CGDCAL0003480. SIT4.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
KOK15427.
OrthoDBEOG7FFN29.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSIT4_CANAL
AccessionPrimary (citable) accession number: Q59KY8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names