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Q59KY8

- SIT4_CANAL

UniProt

Q59KY8 - SIT4_CANAL

Protein

Serine/threonine-protein phosphatase SIT4

Gene

SIT4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Serine/threonine protein phosphatase which is involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1 By similarity. Plays a role during hyphal growth through the regulation of cell wall biogenesis, osmosensing and protein translation. Involved in virulence in a mouse systemic infection model.By similarity2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi55 – 551Manganese 1By similarity
    Metal bindingi85 – 851Manganese 1By similarity
    Metal bindingi85 – 851Manganese 2By similarity
    Metal bindingi117 – 1171Manganese 2By similarity
    Active sitei118 – 1181Proton donorBy similarity
    Metal bindingi167 – 1671Manganese 2By similarity
    Metal bindingi241 – 2411Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: CGD

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: CGD
    2. cellular response to drug Source: CGD
    3. cellular response to neutral pH Source: CGD
    4. filamentous growth Source: CGD
    5. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    6. filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    7. filamentous growth of a population of unicellular organisms in response to heat Source: CGD
    8. filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
    9. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
    10. mitotic nuclear division Source: UniProtKB-KW
    11. pathogenesis Source: CGD
    12. positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    13. positive regulation of filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
    14. positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
    15. protein dephosphorylation Source: CGD
    16. protein phosphorylation Source: CGD
    17. TOR signaling Source: CGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Virulence

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase SIT4 (EC:3.1.3.16)
    Gene namesi
    Name:SIT4
    ORF Names:CaO19.12667, CaO19.5200
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0003480. SIT4.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Causes growth retardation and leads to significant reduction in virulence.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Serine/threonine-protein phosphatase SIT4PRO_0000422107Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with MDS3.1 Publication

    Protein-protein interaction databases

    STRINGi5476.CAL0003480.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59KY8.
    SMRiQ59KY8. Positions 7-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    KOiK15427.
    OrthoDBiEOG7FFN29.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59KY8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGERGPDQWL EQIKNCISLS ESDMKQLCEL VKELLMEESN IQPVQSPVTV    50
    CGDIHGQFHD LLELFRTAGG LPSDDNQTNF IFLGDYVDRG YFSLETFTLL 100
    MVLKVKYPHR ITLVRGNHES RQITQVYGFY EECLTKYGST TVWKYCCQVF 150
    DFLTLAAIID GKILCVHGGL SPEIRMLDQI RVLSRAQEVP HEGGFCDLVW 200
    SDPDNIDTWA VSPRGAGWLF GSKVSREFNH INNLQLIARA HQLVMEGFRY 250
    HFKEKDVVTV WSAPNYCYRC GNVASVMQVD EDLEPNFKIF SAVQDGDLSV 300
    KNNANKQQRS DYFL 314
    Length:314
    Mass (Da):35,899
    Last modified:April 26, 2005 - v1
    Checksum:i9D9577F956F7580F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000273 Genomic DNA. Translation: EAK91146.1.
    AACQ01000272 Genomic DNA. Translation: EAK91157.1.
    RefSeqiXP_710408.1. XM_705316.1.
    XP_710419.1. XM_705327.1.

    Genome annotation databases

    GeneIDi3647982.
    3647993.
    KEGGical:CaO19.12667.
    cal:CaO19.5200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000273 Genomic DNA. Translation: EAK91146.1 .
    AACQ01000272 Genomic DNA. Translation: EAK91157.1 .
    RefSeqi XP_710408.1. XM_705316.1.
    XP_710419.1. XM_705327.1.

    3D structure databases

    ProteinModelPortali Q59KY8.
    SMRi Q59KY8. Positions 7-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0003480.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3647982.
    3647993.
    KEGGi cal:CaO19.12667.
    cal:CaO19.5200.

    Organism-specific databases

    CGDi CAL0003480. SIT4.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    KOi K15427.
    OrthoDBi EOG7FFN29.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    2. "The serine/threonine protein phosphatase SIT4 modulates yeast-to-hypha morphogenesis and virulence in Candida albicans."
      Lee C.M., Nantel A., Jiang L., Whiteway M., Shen S.H.
      Mol. Microbiol. 51:691-709(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    3. "Mds3 regulates morphogenesis in Candida albicans through the TOR pathway."
      Zacchi L.F., Gomez-Raja J., Davis D.A.
      Mol. Cell. Biol. 30:3695-3710(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MDS3.

    Entry informationi

    Entry nameiSIT4_CANAL
    AccessioniPrimary (citable) accession number: Q59KY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3