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Protein

Serine/threonine-protein phosphatase SIT4

Gene

SIT4

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein phosphatase which is involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1 (By similarity). Plays a role during hyphal growth through the regulation of cell wall biogenesis, osmosensing and protein translation. Involved in virulence in a mouse systemic infection model.By similarity2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi55 – 551Manganese 1By similarity
Metal bindingi85 – 851Manganese 1By similarity
Metal bindingi85 – 851Manganese 2By similarity
Metal bindingi117 – 1171Manganese 2By similarity
Active sitei118 – 1181Proton donorBy similarity
Metal bindingi167 – 1671Manganese 2By similarity
Metal bindingi241 – 2411Manganese 2By similarity

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: CGD
  2. metal ion binding Source: UniProtKB-KW
  3. phosphatase activity Source: CGD
  4. protein serine/threonine phosphatase activity Source: CGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. cellular response to cation stress Source: CGD
  3. cellular response to DNA damage stimulus Source: CGD
  4. cellular response to drug Source: CGD
  5. cellular response to neutral pH Source: CGD
  6. cellular response to starvation Source: CGD
  7. DNA damage checkpoint Source: CGD
  8. filamentous growth Source: CGD
  9. filamentous growth of a population of unicellular organisms Source: CGD
  10. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  11. filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  12. filamentous growth of a population of unicellular organisms in response to heat Source: CGD
  13. filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
  14. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  15. fungal-type cell wall organization Source: CGD
  16. hyphal growth Source: CGD
  17. mitotic nuclear division Source: UniProtKB-KW
  18. pathogenesis Source: CGD
  19. positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  20. positive regulation of filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
  21. positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  22. protein dephosphorylation Source: CGD
  23. protein phosphorylation Source: CGD
  24. regulation of apoptotic process Source: CGD
  25. TOR signaling Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Virulence

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase SIT4 (EC:3.1.3.16)
Gene namesi
Name:SIT4
ORF Names:CaO19.12667, CaO19.5200
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0003480. SIT4.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. calcineurin complex Source: CGD
  2. cellular bud neck Source: CGD
  3. cytoplasm Source: UniProtKB-SubCell
  4. nucleus Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes growth retardation and leads to significant reduction in virulence.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Serine/threonine-protein phosphatase SIT4PRO_0000422107Add
BLAST

Interactioni

Subunit structurei

Interacts with MDS3.1 Publication

Protein-protein interaction databases

STRINGi5476.CAL0003480.

Structurei

3D structure databases

ProteinModelPortaliQ59KY8.
SMRiQ59KY8. Positions 7-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ59KY8.
KOiK15427.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59KY8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGERGPDQWL EQIKNCISLS ESDMKQLCEL VKELLMEESN IQPVQSPVTV
60 70 80 90 100
CGDIHGQFHD LLELFRTAGG LPSDDNQTNF IFLGDYVDRG YFSLETFTLL
110 120 130 140 150
MVLKVKYPHR ITLVRGNHES RQITQVYGFY EECLTKYGST TVWKYCCQVF
160 170 180 190 200
DFLTLAAIID GKILCVHGGL SPEIRMLDQI RVLSRAQEVP HEGGFCDLVW
210 220 230 240 250
SDPDNIDTWA VSPRGAGWLF GSKVSREFNH INNLQLIARA HQLVMEGFRY
260 270 280 290 300
HFKEKDVVTV WSAPNYCYRC GNVASVMQVD EDLEPNFKIF SAVQDGDLSV
310
KNNANKQQRS DYFL
Length:314
Mass (Da):35,899
Last modified:April 26, 2005 - v1
Checksum:i9D9577F956F7580F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000273 Genomic DNA. Translation: EAK91146.1.
AACQ01000272 Genomic DNA. Translation: EAK91157.1.
RefSeqiXP_710408.1. XM_705316.1.
XP_710419.1. XM_705327.1.

Genome annotation databases

GeneIDi3647982.
3647993.
KEGGical:CaO19.12667.
cal:CaO19.5200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000273 Genomic DNA. Translation: EAK91146.1.
AACQ01000272 Genomic DNA. Translation: EAK91157.1.
RefSeqiXP_710408.1. XM_705316.1.
XP_710419.1. XM_705327.1.

3D structure databases

ProteinModelPortaliQ59KY8.
SMRiQ59KY8. Positions 7-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5476.CAL0003480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3647982.
3647993.
KEGGical:CaO19.12667.
cal:CaO19.5200.

Organism-specific databases

CGDiCAL0003480. SIT4.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ59KY8.
KOiK15427.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "The serine/threonine protein phosphatase SIT4 modulates yeast-to-hypha morphogenesis and virulence in Candida albicans."
    Lee C.M., Nantel A., Jiang L., Whiteway M., Shen S.H.
    Mol. Microbiol. 51:691-709(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  3. "Mds3 regulates morphogenesis in Candida albicans through the TOR pathway."
    Zacchi L.F., Gomez-Raja J., Davis D.A.
    Mol. Cell. Biol. 30:3695-3710(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MDS3.

Entry informationi

Entry nameiSIT4_CANAL
AccessioniPrimary (citable) accession number: Q59KY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: April 26, 2005
Last modified: February 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.