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Protein

AdoMet-dependent rRNA methyltransferase SPB1

Gene

SPB1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing 2'-O-methylnucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei60S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei78S-adenosyl-L-methionineUniRule annotation1
Binding sitei94S-adenosyl-L-methionineUniRule annotation1
Binding sitei119S-adenosyl-L-methionineUniRule annotation1
Active sitei159Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
AdoMet-dependent rRNA methyltransferase SPB1UniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
2'-O-ribose RNA methyltransferaseUniRule annotation
S-adenosyl-L-methionine-dependent methyltransferaseUniRule annotation
Gene namesi
Name:SPB1UniRule annotation
ORF Names:CaO19.76, CaO19.7727
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000201177. SPB1.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001555931 – 845AdoMet-dependent rRNA methyltransferase SPB1Add BLAST845

Proteomic databases

PRIDEiQ59KF3.

Interactioni

Subunit structurei

Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ59KF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili366 – 402UniRule annotationAdd BLAST37
Coiled coili464 – 502UniRule annotationAdd BLAST39
Coiled coili739 – 796UniRule annotationAdd BLAST58

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi608 – 646Asp-richAdd BLAST39

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ59KF3.
KOiK14857.
OrthoDBiEOG092C4T6A.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E. 1 hit.
MF_03163. RNA_methyltr_E_SPB1. 1 hit.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR028589. Spb1.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 2 hits.
PTHR10920:SF13. PTHR10920:SF13. 2 hits.
PfamiPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59KF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQQKKHSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV
60 70 80 90 100
IDLCAAPGSW CQVASQLCPI NSLIIGVDIV PIKPLPNVIT FQSDITTEDC
110 120 130 140 150
RSRLRGHMKT WKADTVLHDG APNVGLGWVQ DAFTQSQLTL QALKLAVENL
160 170 180 190 200
TAGGTFVTKI FRSRDYNNLM WVFQQLFEKV EATKPPASRN VSAEIFVVCK
210 220 230 240 250
GFKAPKKLDP RLLDPKEVFE ELGGGNESKQ NNEAKIFNPE KFSSQRQRQG
260 270 280 290 300
YQEGDYTLFH TMPIMDFIKQ DDPINQLGSL NKFDLPAPKD DDNDDDDHDH
310 320 330 340 350
EWKILSKLKL CTPELLECIK DLKVLGRKEF KMILKFRKQA RDILGIDKDE
360 370 380 390 400
KEEEEENPKI EVEPLTEEQK IDQELQDLIN KQKQKAKRLK KNANELKQKE
410 420 430 440 450
IIRNQMNMLT DMNIGIEAAQ IGADSLFNLK TAEKTGQLDK LAKGKKKMIF
460 470 480 490 500
NDEELAKDNE IHIDEEEIND NDKDSADELD ELENQLDDMY HQYQARKAER
510 520 530 540 550
DANYRAKQAR GDADDEAWNG IEEDNDDVES GKDYEMESES DDDDDEHIRL
560 570 580 590 600
IAEKKSNGSL SRTARNFFAS DSIFNELSDD VILEEIENKT KGSNGKMVES
610 620 630 640 650
IQEQVANDNN ENDDGDNDEE ESDFEIVPNQ KSDDDDDDES MNSDDDEVST
660 670 680 690 700
TTKSTTHQQK VDLATVEAMT LAHQVALGQK NKYDLIDEGI NRYSFRDKDN
710 720 730 740 750
LPDWFIDDEK KHSKLIKPIT KEAAIAIKEK QKQLNARPIK KVLEAQSRKK
760 770 780 790 800
LRALKRLEKI KKKSDLINED SGKSERDKAD EISKLMKKLN KKQKQKPKTT
810 820 830 840
VVVARGSNRG LSGRPKGVKG KYKMVDGVMK NEQRALKRIA KKYKK
Length:845
Mass (Da):96,820
Last modified:April 26, 2005 - v1
Checksum:i0FA5DE332ECA6767
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti300H → HDH in allele CaO19.7727. 1
Natural varianti351Missing in allele CaO19.7727. 1
Natural varianti514D → DN in allele CaO19.7727. 1
Natural varianti523E → EEEEEE in allele CaO19.7727. 1
Natural varianti618D → DDND in allele CaO19.7727. 1
Natural varianti633 – 634Missing in allele CaO19.7727. 2
Natural varianti642N → D in allele CaO19.7727. 1
Natural varianti747S → G in allele CaO19.7727. 1
Natural varianti843Y → H in allele CaO19.7727. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000302 Genomic DNA. Translation: EAK90959.1.
AACQ01000301 Genomic DNA. Translation: EAK90964.1.
RefSeqiXP_710224.1. XM_705132.1.
XP_710229.1. XM_705137.1.

Genome annotation databases

EnsemblFungiiEAK90959; EAK90959; CaO19.76.
EAK90964; EAK90964; CaO19.7727.
GeneIDi3648174.
3648179.
KEGGical:CaO19.76.
cal:CaO19.7727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000302 Genomic DNA. Translation: EAK90959.1.
AACQ01000301 Genomic DNA. Translation: EAK90964.1.
RefSeqiXP_710224.1. XM_705132.1.
XP_710229.1. XM_705137.1.

3D structure databases

ProteinModelPortaliQ59KF3.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ59KF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK90959; EAK90959; CaO19.76.
EAK90964; EAK90964; CaO19.7727.
GeneIDi3648174.
3648179.
KEGGical:CaO19.76.
cal:CaO19.7727.

Organism-specific databases

CGDiCAL0000201177. SPB1.

Phylogenomic databases

InParanoidiQ59KF3.
KOiK14857.
OrthoDBiEOG092C4T6A.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E. 1 hit.
MF_03163. RNA_methyltr_E_SPB1. 1 hit.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR028589. Spb1.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 2 hits.
PTHR10920:SF13. PTHR10920:SF13. 2 hits.
PfamiPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPB1_CANAL
AccessioniPrimary (citable) accession number: Q59KF3
Secondary accession number(s): Q59KF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: April 26, 2005
Last modified: October 5, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.