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Q59KF3

- SPB1_CANAL

UniProt

Q59KF3 - SPB1_CANAL

Protein

AdoMet-dependent rRNA methyltransferase SPB1

Gene

SPB1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing 2'-O-methylnucleoside.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei58 – 581S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei60 – 601S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei78 – 781S-adenosyl-L-methionineUniRule annotation
    Binding sitei94 – 941S-adenosyl-L-methionineUniRule annotation
    Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
    Active sitei159 – 1591Proton acceptorUniRule annotation

    GO - Molecular functioni

    1. rRNA (guanosine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. enzyme-directed rRNA 2'-O-methylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AdoMet-dependent rRNA methyltransferase SPB1UniRule annotation (EC:2.1.1.-UniRule annotation)
    Alternative name(s):
    2'-O-ribose RNA methyltransferaseUniRule annotation
    S-adenosyl-L-methionine-dependent methyltransferaseUniRule annotation
    Gene namesi
    Name:SPB1UniRule annotation
    ORF Names:CaO19.76, CaO19.7727
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0000659. orf19.76.

    Subcellular locationi

    Nucleusnucleolus UniRule annotation

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. preribosome, large subunit precursor Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 845845AdoMet-dependent rRNA methyltransferase SPB1PRO_0000155593Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle.UniRule annotation

    Protein-protein interaction databases

    STRINGi5476.CAL0000659.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59KF3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili366 – 40237UniRule annotationAdd
    BLAST
    Coiled coili464 – 50239UniRule annotationAdd
    BLAST
    Coiled coili739 – 79658UniRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi608 – 64639Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. RlmE family. SPB1 subfamily.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0293.
    KOiK14857.
    OrthoDBiEOG7P02TJ.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01547. RNA_methyltr_E.
    MF_03163. RNA_methyltr_E_SPB1.
    InterProiIPR015507. rRNA-MeTfrase_E.
    IPR012920. rRNA_MeTfrase_Spb1_C.
    IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    IPR028589. Spb1.
    [Graphical view]
    PANTHERiPTHR10920. PTHR10920. 1 hit.
    PTHR10920:SF13. PTHR10920:SF13. 1 hit.
    PfamiPF11861. DUF3381. 1 hit.
    PF01728. FtsJ. 1 hit.
    PF07780. Spb1_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q59KF3-1 [UniParc]FASTAAdd to Basket

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    MGKQQKKHSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV    50
    IDLCAAPGSW CQVASQLCPI NSLIIGVDIV PIKPLPNVIT FQSDITTEDC 100
    RSRLRGHMKT WKADTVLHDG APNVGLGWVQ DAFTQSQLTL QALKLAVENL 150
    TAGGTFVTKI FRSRDYNNLM WVFQQLFEKV EATKPPASRN VSAEIFVVCK 200
    GFKAPKKLDP RLLDPKEVFE ELGGGNESKQ NNEAKIFNPE KFSSQRQRQG 250
    YQEGDYTLFH TMPIMDFIKQ DDPINQLGSL NKFDLPAPKD DDNDDDDHDH 300
    EWKILSKLKL CTPELLECIK DLKVLGRKEF KMILKFRKQA RDILGIDKDE 350
    KEEEEENPKI EVEPLTEEQK IDQELQDLIN KQKQKAKRLK KNANELKQKE 400
    IIRNQMNMLT DMNIGIEAAQ IGADSLFNLK TAEKTGQLDK LAKGKKKMIF 450
    NDEELAKDNE IHIDEEEIND NDKDSADELD ELENQLDDMY HQYQARKAER 500
    DANYRAKQAR GDADDEAWNG IEEDNDDVES GKDYEMESES DDDDDEHIRL 550
    IAEKKSNGSL SRTARNFFAS DSIFNELSDD VILEEIENKT KGSNGKMVES 600
    IQEQVANDNN ENDDGDNDEE ESDFEIVPNQ KSDDDDDDES MNSDDDEVST 650
    TTKSTTHQQK VDLATVEAMT LAHQVALGQK NKYDLIDEGI NRYSFRDKDN 700
    LPDWFIDDEK KHSKLIKPIT KEAAIAIKEK QKQLNARPIK KVLEAQSRKK 750
    LRALKRLEKI KKKSDLINED SGKSERDKAD EISKLMKKLN KKQKQKPKTT 800
    VVVARGSNRG LSGRPKGVKG KYKMVDGVMK NEQRALKRIA KKYKK 845
    Length:845
    Mass (Da):96,820
    Last modified:April 26, 2005 - v1
    Checksum:i0FA5DE332ECA6767
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti300 – 3001H → HDH in allele CaO19.7727.
    Natural varianti351 – 3511Missing in allele CaO19.7727.
    Natural varianti514 – 5141D → DN in allele CaO19.7727.
    Natural varianti523 – 5231E → EEEEEE in allele CaO19.7727.
    Natural varianti618 – 6181D → DDND in allele CaO19.7727.
    Natural varianti633 – 6342Missing in allele CaO19.7727.
    Natural varianti642 – 6421N → D in allele CaO19.7727.
    Natural varianti747 – 7471S → G in allele CaO19.7727.
    Natural varianti843 – 8431Y → H in allele CaO19.7727.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000302 Genomic DNA. Translation: EAK90959.1.
    AACQ01000301 Genomic DNA. Translation: EAK90964.1.
    RefSeqiXP_710224.1. XM_705132.1.
    XP_710229.1. XM_705137.1.

    Genome annotation databases

    GeneIDi3648174.
    3648179.
    KEGGical:CaO19.76.
    cal:CaO19.7727.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000302 Genomic DNA. Translation: EAK90959.1 .
    AACQ01000301 Genomic DNA. Translation: EAK90964.1 .
    RefSeqi XP_710224.1. XM_705132.1.
    XP_710229.1. XM_705137.1.

    3D structure databases

    ProteinModelPortali Q59KF3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0000659.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3648174.
    3648179.
    KEGGi cal:CaO19.76.
    cal:CaO19.7727.

    Organism-specific databases

    CGDi CAL0000659. orf19.76.

    Phylogenomic databases

    eggNOGi COG0293.
    KOi K14857.
    OrthoDBi EOG7P02TJ.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01547. RNA_methyltr_E.
    MF_03163. RNA_methyltr_E_SPB1.
    InterProi IPR015507. rRNA-MeTfrase_E.
    IPR012920. rRNA_MeTfrase_Spb1_C.
    IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
    IPR002877. rRNA_MeTrfase_FtsJ_dom.
    IPR029063. SAM-dependent_MTases-like.
    IPR028589. Spb1.
    [Graphical view ]
    PANTHERi PTHR10920. PTHR10920. 1 hit.
    PTHR10920:SF13. PTHR10920:SF13. 1 hit.
    Pfami PF11861. DUF3381. 1 hit.
    PF01728. FtsJ. 1 hit.
    PF07780. Spb1_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.

    Entry informationi

    Entry nameiSPB1_CANAL
    AccessioniPrimary (citable) accession number: Q59KF3
    Secondary accession number(s): Q59KF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3