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Q59KF3 (SPB1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AdoMet-dependent rRNA methyltransferase SPB1

EC=2.1.1.-
Alternative name(s):
2'-O-ribose RNA methyltransferase
S-adenosyl-L-methionine-dependent methyltransferase
Gene names
Name:SPB1
ORF Names:CaO19.76, CaO19.7727
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length845 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit By similarity. HAMAP-Rule MF_03163

Catalytic activity

S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing 2'-O-methylnucleoside. HAMAP-Rule MF_03163

Subunit structure

Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle By similarity.

Subcellular location

Nucleusnucleolus By similarity HAMAP-Rule MF_03163.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmE family. SPB1 subfamily.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentNucleus
   DomainCoiled coil
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 845845AdoMet-dependent rRNA methyltransferase SPB1 HAMAP-Rule MF_03163
PRO_0000155593

Regions

Coiled coil366 – 40237 Potential
Coiled coil464 – 50239 Potential
Coiled coil739 – 79658 Potential
Compositional bias608 – 64639Asp-rich HAMAP-Rule MF_03163

Sites

Active site1591Proton acceptor By similarity
Binding site581S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site601S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site781S-adenosyl-L-methionine By similarity
Binding site941S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity

Natural variations

Natural variant3001H → HDH in allele CaO19.7727.
Natural variant3511Missing in allele CaO19.7727.
Natural variant5141D → DN in allele CaO19.7727.
Natural variant5231E → EEEEEE in allele CaO19.7727.
Natural variant6181D → DDND in allele CaO19.7727.
Natural variant633 – 6342Missing in allele CaO19.7727.
Natural variant6421N → D in allele CaO19.7727.
Natural variant7471S → G in allele CaO19.7727.
Natural variant8431Y → H in allele CaO19.7727.

Sequences

Sequence LengthMass (Da)Tools
Q59KF3 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 0FA5DE332ECA6767

FASTA84596,820
        10         20         30         40         50         60 
MGKQQKKHSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV IDLCAAPGSW 

        70         80         90        100        110        120 
CQVASQLCPI NSLIIGVDIV PIKPLPNVIT FQSDITTEDC RSRLRGHMKT WKADTVLHDG 

       130        140        150        160        170        180 
APNVGLGWVQ DAFTQSQLTL QALKLAVENL TAGGTFVTKI FRSRDYNNLM WVFQQLFEKV 

       190        200        210        220        230        240 
EATKPPASRN VSAEIFVVCK GFKAPKKLDP RLLDPKEVFE ELGGGNESKQ NNEAKIFNPE 

       250        260        270        280        290        300 
KFSSQRQRQG YQEGDYTLFH TMPIMDFIKQ DDPINQLGSL NKFDLPAPKD DDNDDDDHDH 

       310        320        330        340        350        360 
EWKILSKLKL CTPELLECIK DLKVLGRKEF KMILKFRKQA RDILGIDKDE KEEEEENPKI 

       370        380        390        400        410        420 
EVEPLTEEQK IDQELQDLIN KQKQKAKRLK KNANELKQKE IIRNQMNMLT DMNIGIEAAQ 

       430        440        450        460        470        480 
IGADSLFNLK TAEKTGQLDK LAKGKKKMIF NDEELAKDNE IHIDEEEIND NDKDSADELD 

       490        500        510        520        530        540 
ELENQLDDMY HQYQARKAER DANYRAKQAR GDADDEAWNG IEEDNDDVES GKDYEMESES 

       550        560        570        580        590        600 
DDDDDEHIRL IAEKKSNGSL SRTARNFFAS DSIFNELSDD VILEEIENKT KGSNGKMVES 

       610        620        630        640        650        660 
IQEQVANDNN ENDDGDNDEE ESDFEIVPNQ KSDDDDDDES MNSDDDEVST TTKSTTHQQK 

       670        680        690        700        710        720 
VDLATVEAMT LAHQVALGQK NKYDLIDEGI NRYSFRDKDN LPDWFIDDEK KHSKLIKPIT 

       730        740        750        760        770        780 
KEAAIAIKEK QKQLNARPIK KVLEAQSRKK LRALKRLEKI KKKSDLINED SGKSERDKAD 

       790        800        810        820        830        840 
EISKLMKKLN KKQKQKPKTT VVVARGSNRG LSGRPKGVKG KYKMVDGVMK NEQRALKRIA 


KKYKK 

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References

[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000302 Genomic DNA. Translation: EAK90959.1.
AACQ01000301 Genomic DNA. Translation: EAK90964.1.
RefSeqXP_710224.1. XM_705132.1.
XP_710229.1. XM_705137.1.

3D structure databases

ProteinModelPortalQ59KF3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0000659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3648174.
3648179.
KEGGcal:CaO19.76.
cal:CaO19.7727.

Organism-specific databases

CGDCAL0000659. orf19.76.

Phylogenomic databases

eggNOGCOG0293.
KOK14857.
OrthoDBEOG7P02TJ.

Family and domain databases

HAMAPMF_01547. RNA_methyltr_E.
MF_03163. RNA_methyltr_E_SPB1.
InterProIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR028589. Spb1.
[Graphical view]
PANTHERPTHR10920. PTHR10920. 1 hit.
PTHR10920:SF13. PTHR10920:SF13. 1 hit.
PfamPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPB1_CANAL
AccessionPrimary (citable) accession number: Q59KF3
Secondary accession number(s): Q59KF8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: April 26, 2005
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names