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Q59KF3

- SPB1_CANAL

UniProt

Q59KF3 - SPB1_CANAL

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Protein

AdoMet-dependent rRNA methyltransferase SPB1

Gene

SPB1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing 2'-O-methylnucleoside.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei60 – 601S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei78 – 781S-adenosyl-L-methionineUniRule annotation
Binding sitei94 – 941S-adenosyl-L-methionineUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Active sitei159 – 1591Proton acceptorUniRule annotation

GO - Molecular functioni

  1. rRNA (guanosine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. enzyme-directed rRNA 2'-O-methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
AdoMet-dependent rRNA methyltransferase SPB1UniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
2'-O-ribose RNA methyltransferaseUniRule annotation
S-adenosyl-L-methionine-dependent methyltransferaseUniRule annotation
Gene namesi
Name:SPB1UniRule annotation
ORF Names:CaO19.76, CaO19.7727
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000659. orf19.76.

Subcellular locationi

Nucleusnucleolus UniRule annotation

GO - Cellular componenti

  1. nucleolus Source: UniProtKB-HAMAP
  2. preribosome, large subunit precursor Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845AdoMet-dependent rRNA methyltransferase SPB1PRO_0000155593Add
BLAST

Interactioni

Subunit structurei

Component of the nucleolar and nucleoplasmic pre-60S ribosomal particle.UniRule annotation

Protein-protein interaction databases

STRINGi5476.CAL0000659.

Structurei

3D structure databases

ProteinModelPortaliQ59KF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili366 – 40237UniRule annotationAdd
BLAST
Coiled coili464 – 50239UniRule annotationAdd
BLAST
Coiled coili739 – 79658UniRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi608 – 64639Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0293.
InParanoidiQ59KF3.
KOiK14857.
OrthoDBiEOG7P02TJ.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
MF_03163. RNA_methyltr_E_SPB1.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases-like.
IPR028589. Spb1.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PTHR10920:SF13. PTHR10920:SF13. 1 hit.
PfamiPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59KF3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKQQKKHSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV
60 70 80 90 100
IDLCAAPGSW CQVASQLCPI NSLIIGVDIV PIKPLPNVIT FQSDITTEDC
110 120 130 140 150
RSRLRGHMKT WKADTVLHDG APNVGLGWVQ DAFTQSQLTL QALKLAVENL
160 170 180 190 200
TAGGTFVTKI FRSRDYNNLM WVFQQLFEKV EATKPPASRN VSAEIFVVCK
210 220 230 240 250
GFKAPKKLDP RLLDPKEVFE ELGGGNESKQ NNEAKIFNPE KFSSQRQRQG
260 270 280 290 300
YQEGDYTLFH TMPIMDFIKQ DDPINQLGSL NKFDLPAPKD DDNDDDDHDH
310 320 330 340 350
EWKILSKLKL CTPELLECIK DLKVLGRKEF KMILKFRKQA RDILGIDKDE
360 370 380 390 400
KEEEEENPKI EVEPLTEEQK IDQELQDLIN KQKQKAKRLK KNANELKQKE
410 420 430 440 450
IIRNQMNMLT DMNIGIEAAQ IGADSLFNLK TAEKTGQLDK LAKGKKKMIF
460 470 480 490 500
NDEELAKDNE IHIDEEEIND NDKDSADELD ELENQLDDMY HQYQARKAER
510 520 530 540 550
DANYRAKQAR GDADDEAWNG IEEDNDDVES GKDYEMESES DDDDDEHIRL
560 570 580 590 600
IAEKKSNGSL SRTARNFFAS DSIFNELSDD VILEEIENKT KGSNGKMVES
610 620 630 640 650
IQEQVANDNN ENDDGDNDEE ESDFEIVPNQ KSDDDDDDES MNSDDDEVST
660 670 680 690 700
TTKSTTHQQK VDLATVEAMT LAHQVALGQK NKYDLIDEGI NRYSFRDKDN
710 720 730 740 750
LPDWFIDDEK KHSKLIKPIT KEAAIAIKEK QKQLNARPIK KVLEAQSRKK
760 770 780 790 800
LRALKRLEKI KKKSDLINED SGKSERDKAD EISKLMKKLN KKQKQKPKTT
810 820 830 840
VVVARGSNRG LSGRPKGVKG KYKMVDGVMK NEQRALKRIA KKYKK
Length:845
Mass (Da):96,820
Last modified:April 26, 2005 - v1
Checksum:i0FA5DE332ECA6767
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti300 – 3001H → HDH in allele CaO19.7727.
Natural varianti351 – 3511Missing in allele CaO19.7727.
Natural varianti514 – 5141D → DN in allele CaO19.7727.
Natural varianti523 – 5231E → EEEEEE in allele CaO19.7727.
Natural varianti618 – 6181D → DDND in allele CaO19.7727.
Natural varianti633 – 6342Missing in allele CaO19.7727.
Natural varianti642 – 6421N → D in allele CaO19.7727.
Natural varianti747 – 7471S → G in allele CaO19.7727.
Natural varianti843 – 8431Y → H in allele CaO19.7727.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000302 Genomic DNA. Translation: EAK90959.1.
AACQ01000301 Genomic DNA. Translation: EAK90964.1.
RefSeqiXP_710224.1. XM_705132.1.
XP_710229.1. XM_705137.1.

Genome annotation databases

GeneIDi3648174.
3648179.
KEGGical:CaO19.76.
cal:CaO19.7727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000302 Genomic DNA. Translation: EAK90959.1 .
AACQ01000301 Genomic DNA. Translation: EAK90964.1 .
RefSeqi XP_710224.1. XM_705132.1.
XP_710229.1. XM_705137.1.

3D structure databases

ProteinModelPortali Q59KF3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0000659.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3648174.
3648179.
KEGGi cal:CaO19.76.
cal:CaO19.7727.

Organism-specific databases

CGDi CAL0000659. orf19.76.

Phylogenomic databases

eggNOGi COG0293.
InParanoidi Q59KF3.
KOi K14857.
OrthoDBi EOG7P02TJ.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01547. RNA_methyltr_E.
MF_03163. RNA_methyltr_E_SPB1.
InterProi IPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases-like.
IPR028589. Spb1.
[Graphical view ]
PANTHERi PTHR10920. PTHR10920. 1 hit.
PTHR10920:SF13. PTHR10920:SF13. 1 hit.
Pfami PF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiSPB1_CANAL
AccessioniPrimary (citable) accession number: Q59KF3
Secondary accession number(s): Q59KF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3