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Protein

RUN domain-containing protein 3A

Gene

RUNDC3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an effector of RAP2A in neuronal cells.By similarity

GO - Molecular functioni

  • GTPase regulator activity Source: ProtInc
  • guanylate cyclase activator activity Source: Ensembl

GO - Biological processi

  • small GTPase mediated signal transduction Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
RUN domain-containing protein 3A
Alternative name(s):
Rap2-interacting protein 8
Short name:
RPIP-8
Gene namesi
Name:RUNDC3A
Synonyms:RAP2IP, RPIP8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:16984. RUNDC3A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162402277.

Polymorphism and mutation databases

BioMutaiRUNDC3A.
DMDMi172045944.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446RUN domain-containing protein 3APRO_0000324155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151PhosphothreonineBy similarity
Modified residuei232 – 2321PhosphoserineBy similarity
Modified residuei416 – 4161PhosphoserineBy similarity
Modified residuei419 – 4191PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ59EK9.
PRIDEiQ59EK9.

PTM databases

PhosphoSiteiQ59EK9.

Expressioni

Gene expression databases

BgeeiQ59EK9.
CleanExiHS_RUNDC3A.
ExpressionAtlasiQ59EK9. baseline and differential.
GenevisibleiQ59EK9. HS.

Organism-specific databases

HPAiHPA023548.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of RAP2A.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9H6143EBI-747225,EBI-10249899
AP1M1Q9BXS54EBI-747225,EBI-541426
ARMC7Q9H6L43EBI-747225,EBI-742909
CCNL2Q96S943EBI-747225,EBI-1045974
DDX6P261963EBI-747225,EBI-351257
EIF3EP602284EBI-747225,EBI-347740
FAM161AQ3B8203EBI-747225,EBI-719941
GEMP550403EBI-747225,EBI-744104
MAGEB4O154813EBI-747225,EBI-751857
MAGOHBQ96A723EBI-747225,EBI-746778
MRPL15Q9P0153EBI-747225,EBI-2371967
RAP2BP612254EBI-747225,EBI-750871
SDCBPO005603EBI-747225,EBI-727004
SYT17Q9BSW73EBI-747225,EBI-745392
TBRG1Q05BL03EBI-747225,EBI-10223693
TFPTG5E9B53EBI-747225,EBI-10178002
ZNF417Q8TAU33EBI-747225,EBI-740727
ZNF587Q96SQ53EBI-747225,EBI-6427977

Protein-protein interaction databases

BioGridi116106. 41 interactions.
IntActiQ59EK9. 20 interactions.
MINTiMINT-1456964.
STRINGi9606.ENSP00000410862.

Structurei

3D structure databases

ProteinModelPortaliQ59EK9.
SMRiQ59EK9. Positions 24-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 189138RUNPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 298298Interaction with RAP2ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili267 – 32256Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the RUNDC3 family.Curated
Contains 1 RUN domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000143407.
HOVERGENiHBG052747.
InParanoidiQ59EK9.
OMAiKSNECLV.
OrthoDBiEOG70CR6K.
PhylomeDBiQ59EK9.
TreeFamiTF323904.

Family and domain databases

InterProiIPR004012. Run_dom.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q59EK9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEASFVQTTM ALGLSSKKAS SRNVAVERKN LITVCRFSVK TLLEKYTAEP
60 70 80 90 100
IDDSSEEFVN FAAILEQILS HRFKACAPAG PVSWFSSDGQ RGFWDYIRLA
110 120 130 140 150
CSKVPNNCVS SIENMENIST ARAKGRAWIR VALMEKRMSE YITTALRDTR
160 170 180 190 200
TTRRFYDSGA IMLRDEATIL TGMLIGLSAI DFSFCLKGEV LDGKTPVVID
210 220 230 240 250
YTPYLKFTQS YDYLTDEEER HSAESSTSED NSPEHPYLPL VTDEDSWYSK
260 270 280 290 300
WHKMEQKFRI VYAQKGYLEE LVRLRESQLK DLEAENRRLQ LQLEEAAAQN
310 320 330 340 350
QREKRELEGV ILELQEQLTG LIPSDHAPLA QGSKELTTPL VNQWPSLGTL
360 370 380 390 400
NGAEGASNSK LYRRHSFMST EPLSAEASLS SDSQRLGEGT RDEEPWGPIG
410 420 430 440
KDPTPSMLGL CGSLASIPSC KSLASFKSNE CLVSDSPEGS PALSPS
Length:446
Mass (Da):49,747
Last modified:March 18, 2008 - v2
Checksum:i2C753EA8619B1024
GO
Isoform 2 (identifier: Q59EK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-79: Missing.
     401-405: KDPTP → SSEPN
     406-446: Missing.

Show »
Length:400
Mass (Da):45,269
Checksum:iA240F80E209EDB28
GO
Isoform 3 (identifier: Q59EK9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     401-405: KDPTP → SSEPN
     406-446: Missing.

Show »
Length:405
Mass (Da):45,682
Checksum:i4D39EDF3A180023A
GO
Isoform 4 (identifier: Q59EK9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     365-446: Missing.

Show »
Length:364
Mass (Da):41,324
Checksum:iDE4CDEBD8C6085CF
GO

Sequence cautioni

The sequence BAD93039.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341M → V in CAD97685 (PubMed:17974005).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei75 – 795Missing in isoform 2. 2 PublicationsVSP_032153
Alternative sequencei365 – 44682Missing in isoform 4. 1 PublicationVSP_032154Add
BLAST
Alternative sequencei401 – 4055KDPTP → SSEPN in isoform 2 and isoform 3. 6 PublicationsVSP_032155
Alternative sequencei406 – 44641Missing in isoform 2 and isoform 3. 6 PublicationsVSP_032156Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93871 mRNA. Translation: AAB68767.1.
AF055026 mRNA. Translation: AAC09366.1.
AK313669 mRNA. Translation: BAG36421.1.
CR456748 mRNA. Translation: CAG33029.1.
AB209802 mRNA. Translation: BAD93039.1. Different initiation.
BX537443 mRNA. Translation: CAD97685.1.
AL133657 mRNA. Translation: CAB63771.1.
CH471178 Genomic DNA. Translation: EAW51609.1.
CH471178 Genomic DNA. Translation: EAW51610.1.
BC006194 mRNA. Translation: AAH06194.1.
CCDSiCCDS45698.1. [Q59EK9-1]
CCDS45699.1. [Q59EK9-3]
CCDS59294.1. [Q59EK9-2]
RefSeqiNP_001138297.1. NM_001144825.1. [Q59EK9-1]
NP_001138298.1. NM_001144826.1. [Q59EK9-2]
NP_006686.1. NM_006695.4. [Q59EK9-3]
UniGeneiHs.500197.

Genome annotation databases

EnsembliENST00000225441; ENSP00000225441; ENSG00000108309. [Q59EK9-3]
ENST00000426726; ENSP00000410862; ENSG00000108309. [Q59EK9-1]
ENST00000590941; ENSP00000468214; ENSG00000108309. [Q59EK9-2]
GeneIDi10900.
KEGGihsa:10900.
UCSCiuc002igi.4. human. [Q59EK9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93871 mRNA. Translation: AAB68767.1.
AF055026 mRNA. Translation: AAC09366.1.
AK313669 mRNA. Translation: BAG36421.1.
CR456748 mRNA. Translation: CAG33029.1.
AB209802 mRNA. Translation: BAD93039.1. Different initiation.
BX537443 mRNA. Translation: CAD97685.1.
AL133657 mRNA. Translation: CAB63771.1.
CH471178 Genomic DNA. Translation: EAW51609.1.
CH471178 Genomic DNA. Translation: EAW51610.1.
BC006194 mRNA. Translation: AAH06194.1.
CCDSiCCDS45698.1. [Q59EK9-1]
CCDS45699.1. [Q59EK9-3]
CCDS59294.1. [Q59EK9-2]
RefSeqiNP_001138297.1. NM_001144825.1. [Q59EK9-1]
NP_001138298.1. NM_001144826.1. [Q59EK9-2]
NP_006686.1. NM_006695.4. [Q59EK9-3]
UniGeneiHs.500197.

3D structure databases

ProteinModelPortaliQ59EK9.
SMRiQ59EK9. Positions 24-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116106. 41 interactions.
IntActiQ59EK9. 20 interactions.
MINTiMINT-1456964.
STRINGi9606.ENSP00000410862.

PTM databases

PhosphoSiteiQ59EK9.

Polymorphism and mutation databases

BioMutaiRUNDC3A.
DMDMi172045944.

Proteomic databases

PaxDbiQ59EK9.
PRIDEiQ59EK9.

Protocols and materials databases

DNASUi10900.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225441; ENSP00000225441; ENSG00000108309. [Q59EK9-3]
ENST00000426726; ENSP00000410862; ENSG00000108309. [Q59EK9-1]
ENST00000590941; ENSP00000468214; ENSG00000108309. [Q59EK9-2]
GeneIDi10900.
KEGGihsa:10900.
UCSCiuc002igi.4. human. [Q59EK9-1]

Organism-specific databases

CTDi10900.
GeneCardsiRUNDC3A.
HGNCiHGNC:16984. RUNDC3A.
HPAiHPA023548.
MIMi605448. gene.
neXtProtiNX_Q59EK9.
PharmGKBiPA162402277.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000143407.
HOVERGENiHBG052747.
InParanoidiQ59EK9.
OMAiKSNECLV.
OrthoDBiEOG70CR6K.
PhylomeDBiQ59EK9.
TreeFamiTF323904.

Miscellaneous databases

ChiTaRSiRUNDC3A. human.
GeneWikiiRUNDC3A.
GenomeRNAii10900.
NextBioi41395.
PROiQ59EK9.
SOURCEiSearch...

Gene expression databases

BgeeiQ59EK9.
CleanExiHS_RUNDC3A.
ExpressionAtlasiQ59EK9. baseline and differential.
GenevisibleiQ59EK9. HS.

Family and domain databases

InterProiIPR004012. Run_dom.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a specific effector of the small GTP-binding protein Rap2."
    Janoueix-Lerosey I., Pasheva E., de Tand M.-F., Tavitian A., de Gunzburg J.
    Eur. J. Biochem. 252:290-298(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION (ISOFORM 1).
  2. Yu W., Gibbs R.A.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Caudate nucleus.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Tissue: Cerebellum and Testis.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Lung.

Entry informationi

Entry nameiRUN3A_HUMAN
AccessioniPrimary (citable) accession number: Q59EK9
Secondary accession number(s): B2R974
, O15483, O60651, Q7Z3S2, Q9UF50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: March 16, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.