Q59E36 (RCOR_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
REST corepressor

Alternative name(s):
CoREST

Gene names

Name:	CoRest
ORF Names:	CG33525

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited by Ttk88 and probably acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. May serve as a molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval. Ref.5
Subunit structure	Component of a complex that contains at least Rpd3, CoRest and Su(var)3-3/Hdm. Interacts with neuronal repressor ttk/Ttk88. Ref.5
Subcellular location	Nucleus Ref.5.
Tissue specificity	Present in all of the tissues examined including both glia and neurons of the CNS (at protein level). Expressed ubiquitously in the embryo. In contrast, expression of isoform 3 is highly enriched in the nervous system. Ref.5
Sequence similarities	Belongs to the CoREST family. Contains 1 ELM2 domain. Contains 2 SANT domains.
Sequence caution	The sequence AAL25265.1 differs from that shown. Reason: Erroneous initiation. The sequence AAM49876.1 differs from that shown. Reason: Erroneous initiation. The sequence AAX52508.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of histone H3-K27 methylation Inferred from mutant phenotype PubMed 22331351. Source: FlyBase negative regulation of histone H4-K16 acetylation Inferred from mutant phenotype PubMed 22331351. Source: FlyBase negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.5. Source: FlyBase positive regulation of Notch signaling pathway Inferred from mutant phenotype PubMed 22331351. Source: FlyBase positive regulation of transcription of Notch receptor target Inferred from mutant phenotype PubMed 22331351. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcriptional repressor complex Inferred from physical interaction Ref.5. Source: FlyBase
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro chromatin binding Inferred from direct assay Ref.5. Source: FlyBase transcription corepressor activity Inferred from direct assay Ref.5. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q59E36-1)

Also known as: F; dCRL;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q59E36-2)

Also known as: E;

The sequence of this isoform differs from the canonical sequence as follows:
     265-333: SKPLQTADANTAIGRRTGNRRPISGPEGNRKPPKGMYINHDDLTALASCGNPSLYLAERERKLTALMAE → IQ

Isoform 3 (identifier: Q59E36-3)

Also known as: A; C; D; dCRS;

The sequence of this isoform differs from the canonical sequence as follows:
     265-273: SKPLQTADA → IIAVPAHIS
     274-657: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	REST corepressor		PRO_0000226784
Regions
	Domain	90 – 175	86	ELM2
	Domain	176 – 227	52	SANT 1
	Domain	369 – 420	52	SANT 2
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.7
	Modified residue	22	1	Phosphoserine Ref.6 Ref.7
	Modified residue	25	1	Phosphothreonine Ref.7
	Modified residue	34	1	Phosphoserine Ref.6
	Modified residue	36	1	Phosphoserine Ref.6
	Modified residue	65	1	Phosphothreonine Ref.7
	Modified residue	68	1	Phosphoserine Ref.7
	Modified residue	69	1	Phosphoserine Ref.7
	Modified residue	442	1	Phosphothreonine Ref.7
	Modified residue	478	1	Phosphothreonine Ref.7
	Modified residue	481	1	Phosphoserine Ref.7
	Modified residue	627	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	265 – 333	69	SKPLQ…ALMAE → IQ in isoform 2.		VSP_017465
	Alternative sequence	265 – 273	9	SKPLQTADA → IIAVPAHIS in isoform 3.		VSP_017466
	Alternative sequence	274 – 657	384	Missing in isoform 3.		VSP_017467

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (F) (dCRL) [UniParc]. Last modified April 26, 2005. Version 1. Checksum: AD86BCE9EA31E9D9	FASTA	657	69,929
	10 20 30 40 50 60 MVLAERNTTD VVRNGRRSRG PSPNTHTTGG VTNSASLVGS GNNSGHSGNA NANEKTTTAV 70 80 90 100 110 120 PGAGTPESSD DDNSTKRNGK SKAKQSEYEE KIRVGRDYQA VCPPLVPEAE RRPEQMNERA 130 140 150 160 170 180 LLVWSPTKEI PDLKLEEYIS VAKEKYGYNG EQALGMLFWH KHDLERAVMD LANFTPFPDE 190 200 210 220 230 240 WTIEDKVLFE QAFQFHGKSF HRIRQMLPDK SIASLVKYYY SWKKTRHRSS AMDRQEKAIK 250 260 270 280 290 300 AVVKDGSENG SEVGSNEESD NDDKSKPLQT ADANTAIGRR TGNRRPISGP EGNRKPPKGM 310 320 330 340 350 360 YINHDDLTAL ASCGNPSLYL AERERKLTAL MAEKNRQVME QLDKECETIN VDDVLSKPAA 370 380 390 400 410 420 ANTESAQPRI SARWLPDEIQ VALLAIREYG KNFPTIAKVV ATKTEAHVRT FYLNNRRRYN 430 440 450 460 470 480 LDQIVKEYEA GKSEESGAEE QTEPAVDAAA AGAATASAPS AADSTSAASA TADRKQSTEN 490 500 510 520 530 540 SNNGVETMQE SLPKKEDPKK PELAAAVLKI GVAEAADAVA TVASSTASGV IGAATSASKP 550 560 570 580 590 600 STSATITIID ESDTATNSSS DVVTTGLATS TGSSALSSTT SAPASKTQTS SEELSAQKSN 610 620 630 640 650 PASGIAAIGA ATGGGQTANS SGSKRDSSVL PVAEQPPAKK IALSTGGGSS VAEFLAN
	Isoform 2 (E) [UniParc]. Checksum: 17B010081DB57AD7	FASTA	590	62,696
	10 20 30 40 50 60 MVLAERNTTD VVRNGRRSRG PSPNTHTTGG VTNSASLVGS GNNSGHSGNA NANEKTTTAV 70 80 90 100 110 120 PGAGTPESSD DDNSTKRNGK SKAKQSEYEE KIRVGRDYQA VCPPLVPEAE RRPEQMNERA 130 140 150 160 170 180 LLVWSPTKEI PDLKLEEYIS VAKEKYGYNG EQALGMLFWH KHDLERAVMD LANFTPFPDE 190 200 210 220 230 240 WTIEDKVLFE QAFQFHGKSF HRIRQMLPDK SIASLVKYYY SWKKTRHRSS AMDRQEKAIK 250 260 270 280 290 300 AVVKDGSENG SEVGSNEESD NDDKIQKNRQ VMEQLDKECE TINVDDVLSK PAAANTESAQ 310 320 330 340 350 360 PRISARWLPD EIQVALLAIR EYGKNFPTIA KVVATKTEAH VRTFYLNNRR RYNLDQIVKE 370 380 390 400 410 420 YEAGKSEESG AEEQTEPAVD AAAAGAATAS APSAADSTSA ASATADRKQS TENSNNGVET 430 440 450 460 470 480 MQESLPKKED PKKPELAAAV LKIGVAEAAD AVATVASSTA SGVIGAATSA SKPSTSATIT 490 500 510 520 530 540 IIDESDTATN SSSDVVTTGL ATSTGSSALS STTSAPASKT QTSSEELSAQ KSNPASGIAA 550 560 570 580 590 IGAATGGGQT ANSSGSKRDS SVLPVAEQPP AKKIALSTGG GSSVAEFLAN
	Isoform 3 (A) (C) (D) (dCRS) [UniParc]. Checksum: 348FE8771C69BC45	FASTA	273	30,468
	10 20 30 40 50 60 MVLAERNTTD VVRNGRRSRG PSPNTHTTGG VTNSASLVGS GNNSGHSGNA NANEKTTTAV 70 80 90 100 110 120 PGAGTPESSD DDNSTKRNGK SKAKQSEYEE KIRVGRDYQA VCPPLVPEAE RRPEQMNERA 130 140 150 160 170 180 LLVWSPTKEI PDLKLEEYIS VAKEKYGYNG EQALGMLFWH KHDLERAVMD LANFTPFPDE 190 200 210 220 230 240 WTIEDKVLFE QAFQFHGKSF HRIRQMLPDK SIASLVKYYY SWKKTRHRSS AMDRQEKAIK 250 260 270 AVVKDGSENG SEVGSNEESD NDDKIIAVPA HIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Embryo.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-657 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-657 (ISOFORM 1/2). Strain: Berkeley. Tissue: Embryo and Head.
[5]	"A conserved role but different partners for the transcriptional corepressor CoREST in fly and mammalian nervous system formation." Dallman J.E., Allopenna J., Bassett A., Travers A., Mandel G. J. Neurosci. 24:7186-7193(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH TTK; RPD3 AND SU(VAR)3-3.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-34; SER-36 AND SER-627, MASS SPECTROMETRY.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-22; THR-25; THR-65; SER-68; SER-69; THR-442; THR-478 AND SER-481, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF48966.1. AE014298 Genomic DNA. Translation: AAN09497.1. AE014298 Genomic DNA. Translation: AAN09498.1. AE014298 Genomic DNA. Translation: AAX52507.1. AE014298 Genomic DNA. Translation: AAX52508.1. Sequence problems. BT023817 mRNA. Translation: AAZ66324.1. AY060226 mRNA. Translation: AAL25265.1. Different initiation. AY118507 mRNA. Translation: AAM49876.1. Different initiation.
RefSeq	NP_001014752.2. NM_001014752.4. NP_001014753.1. NM_001014753.3. NP_001014754.1. NM_001014754.1. NP_001014755.1. NM_001014755.2. NP_001014756.1. NM_001014756.2.
UniGene	Dm.4085.
3D structure databases
ProteinModelPortal	Q59E36.
SMR	Q59E36. Positions 179-225, 294-430.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q59E36. 2 interactions.
MINT	MINT-1651349.
Proteomic databases
PaxDb	Q59E36.
PRIDE	Q59E36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	32941.
KEGG	dme:Dmel_CG42687.
UCSC	CG33525-RA. d. melanogaster.
Organism-specific databases
CTD	32941.
FlyBase	FBgn0261573. CoRest.
Phylogenomic databases
eggNOG	NOG326181.
InParanoid	Q59E36.
OrthoDB	EOG46DJJD.
PhylomeDB	Q59E36.
Gene expression databases
GermOnline	CG33525. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000949. ELM2_dom. IPR009057. Homeodomain-like. IPR001005. SANT/Myb. IPR017884. SANT_dom. [Graphical view]
Pfam	PF01448. ELM2. 1 hit. [Graphical view]
SMART	SM00717. SANT. 2 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS51156. ELM2. 1 hit. PS51293. SANT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32941.
NextBio	781152.

Entry information

Entry name

RCOR_DROME

Accession

Primary (citable) accession number: Q59E36
Secondary accession number(s): A4V4S3 , Q494F9, Q59E35, Q8MSX7, Q95TC9, Q9VWH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project