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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (PPAT), Amidophosphoribosyltransferase (PPAT)
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288ManganeseBy similarity1
Metal bindingi290ManganeseBy similarity1
Binding sitei87110-formyltetrahydrofolateBy similarity1
Binding sitei91310-formyltetrahydrofolateBy similarity1
Active sitei915Proton donorBy similarity1
Sitei951Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 199ATPBy similarityAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002507152 – 1010Trifunctional purine biosynthetic protein adenosine-3Add BLAST1009

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei350N6-acetyllysineBy similarity1
Modified residuei440PhosphoserineBy similarity1
Modified residuei682PhosphothreonineBy similarity1
Modified residuei802PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ59A32.
PeptideAtlasiQ59A32.
PRIDEiQ59A32.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009188.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108.

Structurei

3D structure databases

ProteinModelPortaliQ59A32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 318ATP-graspAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni434 – 809AIRSAdd BLAST376
Regioni810 – 1010GARTAdd BLAST201
Regioni818 – 8205'-phosphoribosylglycinamide bindingBy similarity3
Regioni896 – 89910-formyltetrahydrofolate bindingBy similarity4
Regioni947 – 95110-formyltetrahydrofolate bindingBy similarity5
Regioni977 – 9805'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiKOG0237. Eukaryota.
KOG3076. Eukaryota.
COG0150. LUCA.
COG0151. LUCA.
COG0299. LUCA.
GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiQ59A32.
KOiK11787.
OMAiYRTDIAQ.
OrthoDBiEOG091G026D.
TreeFamiTF106368.

Family and domain databases

CDDicd08645. FMT_core_GART. 1 hit.
cd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
MF_01930. PurN. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR004607. PurN.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI
60 70 80 90 100
SISDHTALAQ FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA
110 120 130 140 150
AQLESSKRFA KEFMDRHGIS TARWRAFTKP KEACDFIMSA DFPALVVKAS
160 170 180 190 200
GLAAGKGVIV AKSKEEACEA VREIMQGKAF GEAGETVVIE ELLEGEEVSC
210 220 230 240 250
LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP QVSKDLLLKI
260 270 280 290 300
KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK
360 370 380 390 400
GVEITGFPEA QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL
410 420 430 440 450
EEARKGLAAI KFEGAVYRKD IGFRAIAFLQ QPRGLTYKES GVDIAAGNML
460 470 480 490 500
VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK AAGFTDPLLA CGTDGVGTKL
510 520 530 540 550
KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC GKLDLRTTEA
560 570 580 590 600
VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP
610 620 630 640 650
QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT
660 670 680 690 700
LGDLLLTPTK IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV
710 720 730 740 750
NLDAQTWRVP RIFSWLQQEG HLSEEEMART FNCGIGAALV VSEDLVKQTL
760 770 780 790 800
QDIEQHQEEA CVIGRVVACP KGSPRVKVEH LIETMQINGS VLENGTLRNH
810 820 830 840 850
FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV ISNKAAVAGL
860 870 880 890 900
DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS
910 920 930 940 950
GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV
960 970 980 990 1000
DAGQIILQEA VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE
1010
NGRICWVTED
Length:1,010
Mass (Da):107,907
Last modified:April 26, 2005 - v1
Checksum:i27DFCA438661A771
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti771K → E.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1.
BC122573 mRNA. Translation: AAI22574.1.
RefSeqiNP_001035563.1. NM_001040473.2.
UniGeneiBt.11270.
Bt.94801.

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
GeneIDi281183.
KEGGibta:281183.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1.
BC122573 mRNA. Translation: AAI22574.1.
RefSeqiNP_001035563.1. NM_001040473.2.
UniGeneiBt.11270.
Bt.94801.

3D structure databases

ProteinModelPortaliQ59A32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108.

Proteomic databases

PaxDbiQ59A32.
PeptideAtlasiQ59A32.
PRIDEiQ59A32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
GeneIDi281183.
KEGGibta:281183.

Organism-specific databases

CTDi2618.

Phylogenomic databases

eggNOGiKOG0237. Eukaryota.
KOG3076. Eukaryota.
COG0150. LUCA.
COG0151. LUCA.
COG0299. LUCA.
GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiQ59A32.
KOiK11787.
OMAiYRTDIAQ.
OrthoDBiEOG091G026D.
TreeFamiTF106368.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.
ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.

Gene expression databases

BgeeiENSBTAG00000009188.

Family and domain databases

CDDicd08645. FMT_core_GART. 1 hit.
cd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
MF_01930. PurN. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR004607. PurN.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR2_BOVIN
AccessioniPrimary (citable) accession number: Q59A32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: April 26, 2005
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.