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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (PPAT), Amidophosphoribosyltransferase (PPAT)
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881ManganeseBy similarity
Metal bindingi290 – 2901ManganeseBy similarity
Binding sitei871 – 871110-formyltetrahydrofolateBy similarity
Binding sitei913 – 913110-formyltetrahydrofolateBy similarity
Active sitei915 – 9151Proton donorBy similarity
Sitei951 – 9511Raises pKa of active site HisBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 19963ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_321076. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 10101009Trifunctional purine biosynthetic protein adenosine-3PRO_0000250715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei350 – 3501N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ59A32.
PRIDEiQ59A32.

Expressioni

Gene expression databases

ExpressionAtlasiQ59A32. baseline.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108.

Structurei

3D structure databases

ProteinModelPortaliQ59A32.
SMRiQ59A32. Positions 1-430, 475-792, 808-1007.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 318208ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 809376AIRSAdd
BLAST
Regioni810 – 1010201GARTAdd
BLAST
Regioni818 – 82035'-phosphoribosylglycinamide bindingBy similarity
Regioni896 – 899410-formyltetrahydrofolate bindingBy similarity
Regioni947 – 951510-formyltetrahydrofolate bindingBy similarity
Regioni977 – 98045'-phosphoribosylglycinamide bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiQ59A32.
KOiK11787.
OMAiSGCNAEL.
OrthoDBiEOG7RBZ7H.
TreeFamiTF106368.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI
60 70 80 90 100
SISDHTALAQ FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA
110 120 130 140 150
AQLESSKRFA KEFMDRHGIS TARWRAFTKP KEACDFIMSA DFPALVVKAS
160 170 180 190 200
GLAAGKGVIV AKSKEEACEA VREIMQGKAF GEAGETVVIE ELLEGEEVSC
210 220 230 240 250
LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP QVSKDLLLKI
260 270 280 290 300
KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK
360 370 380 390 400
GVEITGFPEA QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL
410 420 430 440 450
EEARKGLAAI KFEGAVYRKD IGFRAIAFLQ QPRGLTYKES GVDIAAGNML
460 470 480 490 500
VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK AAGFTDPLLA CGTDGVGTKL
510 520 530 540 550
KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC GKLDLRTTEA
560 570 580 590 600
VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP
610 620 630 640 650
QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT
660 670 680 690 700
LGDLLLTPTK IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV
710 720 730 740 750
NLDAQTWRVP RIFSWLQQEG HLSEEEMART FNCGIGAALV VSEDLVKQTL
760 770 780 790 800
QDIEQHQEEA CVIGRVVACP KGSPRVKVEH LIETMQINGS VLENGTLRNH
810 820 830 840 850
FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV ISNKAAVAGL
860 870 880 890 900
DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS
910 920 930 940 950
GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV
960 970 980 990 1000
DAGQIILQEA VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE
1010
NGRICWVTED
Length:1,010
Mass (Da):107,907
Last modified:April 26, 2005 - v1
Checksum:i27DFCA438661A771
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti771 – 7711K → E.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1.
BC122573 mRNA. Translation: AAI22574.1.
RefSeqiNP_001035563.1. NM_001040473.2.
UniGeneiBt.11270.
Bt.94801.

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
GeneIDi281183.
KEGGibta:281183.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1.
BC122573 mRNA. Translation: AAI22574.1.
RefSeqiNP_001035563.1. NM_001040473.2.
UniGeneiBt.11270.
Bt.94801.

3D structure databases

ProteinModelPortaliQ59A32.
SMRiQ59A32. Positions 1-430, 475-792, 808-1007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108.

Proteomic databases

PaxDbiQ59A32.
PRIDEiQ59A32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
GeneIDi281183.
KEGGibta:281183.

Organism-specific databases

CTDi2618.

Phylogenomic databases

eggNOGiCOG0151.
GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiQ59A32.
KOiK11787.
OMAiSGCNAEL.
OrthoDBiEOG7RBZ7H.
TreeFamiTF106368.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.
ReactomeiREACT_321076. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi20805240.

Gene expression databases

ExpressionAtlasiQ59A32. baseline.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2."
    Woehlke A., Droegemueller C., Kuiper H., Leeb T., Distl O.
    Gene 348:73-81(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-771.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiPUR2_BOVIN
AccessioniPrimary (citable) accession number: Q59A32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: April 26, 2005
Last modified: May 27, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.