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Q59A32

- PUR2_BOVIN

UniProt

Q59A32 - PUR2_BOVIN

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Protein
Trifunctional purine biosynthetic protein adenosine-3
Gene
GART
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881Manganese By similarity
Metal bindingi290 – 2901Manganese By similarity
Binding sitei871 – 871110-formyltetrahydrofolate By similarity
Binding sitei913 – 913110-formyltetrahydrofolate By similarity
Active sitei915 – 9151Proton donor By similarity
Sitei951 – 9511Raises pKa of active site His By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 19963ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_226478. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 10101009Trifunctional purine biosynthetic protein adenosine-3UniRule annotation
PRO_0000250715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei350 – 3501N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ59A32.
PRIDEiQ59A32.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108.

Structurei

3D structure databases

ProteinModelPortaliQ59A32.
SMRiQ59A32. Positions 1-430, 475-792, 808-1007.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 318208ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 809376AIRSUniRule annotation
Add
BLAST
Regioni810 – 1010201GARTUniRule annotation
Add
BLAST
Regioni818 – 82035'-phosphoribosylglycinamide binding By similarity
Regioni896 – 899410-formyltetrahydrofolate binding By similarity
Regioni947 – 951510-formyltetrahydrofolate binding By similarity
Regioni977 – 98045'-phosphoribosylglycinamide binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiQ59A32.
KOiK11787.
OMAiGMLFTGF.
OrthoDBiEOG7RBZ7H.
TreeFamiTF106368.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59A32-1 [UniParc]FASTAAdd to Basket

« Hide

MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI     50
SISDHTALAQ FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA 100
AQLESSKRFA KEFMDRHGIS TARWRAFTKP KEACDFIMSA DFPALVVKAS 150
GLAAGKGVIV AKSKEEACEA VREIMQGKAF GEAGETVVIE ELLEGEEVSC 200
LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP QVSKDLLLKI 250
KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV 300
ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK 350
GVEITGFPEA QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL 400
EEARKGLAAI KFEGAVYRKD IGFRAIAFLQ QPRGLTYKES GVDIAAGNML 450
VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK AAGFTDPLLA CGTDGVGTKL 500
KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC GKLDLRTTEA 550
VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP 600
QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT 650
LGDLLLTPTK IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV 700
NLDAQTWRVP RIFSWLQQEG HLSEEEMART FNCGIGAALV VSEDLVKQTL 750
QDIEQHQEEA CVIGRVVACP KGSPRVKVEH LIETMQINGS VLENGTLRNH 800
FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV ISNKAAVAGL 850
DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS 900
GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV 950
DAGQIILQEA VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE 1000
NGRICWVTED 1010
Length:1,010
Mass (Da):107,907
Last modified:April 26, 2005 - v1
Checksum:i27DFCA438661A771
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti771 – 7711K → E.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1.
BC122573 mRNA. Translation: AAI22574.1.
RefSeqiNP_001035563.1. NM_001040473.2.
XP_005201140.1. XM_005201083.1.
XP_005201141.1. XM_005201084.1.
UniGeneiBt.11270.
Bt.94801.

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
GeneIDi281183.
KEGGibta:281183.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ780930 Genomic DNA. Translation: CAG47113.1 .
BC122573 mRNA. Translation: AAI22574.1 .
RefSeqi NP_001035563.1. NM_001040473.2.
XP_005201140.1. XM_005201083.1.
XP_005201141.1. XM_005201084.1.
UniGenei Bt.11270.
Bt.94801.

3D structure databases

ProteinModelPortali Q59A32.
SMRi Q59A32. Positions 1-430, 475-792, 808-1007.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000012108.

Proteomic databases

PaxDbi Q59A32.
PRIDEi Q59A32.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000012108 ; ENSBTAP00000012108 ; ENSBTAG00000009188 .
GeneIDi 281183.
KEGGi bta:281183.

Organism-specific databases

CTDi 2618.

Phylogenomic databases

eggNOGi COG0151.
GeneTreei ENSGT00390000000292.
HOGENOMi HOG000030315.
HOVERGENi HBG008333.
InParanoidi Q59A32.
KOi K11787.
OMAi GMLFTGF.
OrthoDBi EOG7RBZ7H.
TreeFami TF106368.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .
Reactomei REACT_226478. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi 20805240.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2."
    Woehlke A., Droegemueller C., Kuiper H., Leeb T., Distl O.
    Gene 348:73-81(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-771.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiPUR2_BOVIN
AccessioniPrimary (citable) accession number: Q59A32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: April 26, 2005
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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