Reviewed, UniProtKB/Swiss-Prot Q59A32 (PUR2_BOVIN)

Names and origin

Protein names

Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
    1- Recommended name:
            Phosphoribosylamine--glycine ligase
              EC=6.3.4.13
        Alternative name(s):
            Glycinamide ribonucleotide synthetase
              Short name=GARS
            Phosphoribosylglycinamide synthetase
    2- Recommended name:
            Phosphoribosylformylglycinamidine cyclo-ligase
              EC=6.3.3.1
        Alternative name(s):
            Phosphoribosyl-aminoimidazole synthetase
            AIR synthase
              Short name=AIRS
    3- Recommended name:
            Phosphoribosylglycinamide formyltransferase
              EC=2.1.2.2
        Alternative name(s):
            5'-phosphoribosylglycinamide transformylase
            GAR transformylase
              Short name=GART

Gene names

Name:

GART

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1010 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	In the N-terminal section; belongs to the GARS family. In the central section; belongs to the AIR synthase family. In the C-terminal section; belongs to the GART family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1010	1009	Trifunctional purine biosynthetic protein adenosine-3		PRO_0000250715
Regions
	Domain	111 – 318	208	ATP-grasp
	Nucleotide binding	137 – 199	63	ATP By similarity
	Region	434 – 809	376	AIRS
	Region	810 – 1010	201	GART
	Region	818 – 820	3	5'-phosphoribosylglycinamide binding By similarity
	Region	896 – 899	4	10-formyltetrahydrofolate binding By similarity
	Region	947 – 951	5	10-formyltetrahydrofolate binding By similarity
	Region	977 – 980	4	5'-phosphoribosylglycinamide binding By similarity
Sites
	Active site	915	1	Proton donor By similarity
	Metal binding	288	1	Manganese By similarity
	Metal binding	290	1	Manganese By similarity
	Binding site	871	1	10-formyltetrahydrofolate By similarity
	Binding site	913	1	10-formyltetrahydrofolate By similarity
	Site	951	1	Raises pKa of active site His By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	348	1	Phosphotyrosine By similarity
	Modified residue	350	1	N6-acetyllysine By similarity
Natural variations
	Natural variant	771	1	K → E

Sequences

	Sequence		Length	Mass (Da)
	Q59A32-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 27DFCA438661A771	FASTA	1,010	107,907
	10 20 30 40 50 60 MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI SISDHTALAQ 70 80 90 100 110 120 FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA AQLESSKRFA KEFMDRHGIS 130 140 150 160 170 180 TARWRAFTKP KEACDFIMSA DFPALVVKAS GLAAGKGVIV AKSKEEACEA VREIMQGKAF 190 200 210 220 230 240 GEAGETVVIE ELLEGEEVSC LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP 250 260 270 280 290 300 QVSKDLLLKI KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV 310 320 330 340 350 360 ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK GVEITGFPEA 370 380 390 400 410 420 QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL EEARKGLAAI KFEGAVYRKD 430 440 450 460 470 480 IGFRAIAFLQ QPRGLTYKES GVDIAAGNML VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK 490 500 510 520 530 540 AAGFTDPLLA CGTDGVGTKL KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC 550 560 570 580 590 600 GKLDLRTTEA VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP 610 620 630 640 650 660 QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT LGDLLLTPTK 670 680 690 700 710 720 IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV NLDAQTWRVP RIFSWLQQEG 730 740 750 760 770 780 HLSEEEMART FNCGIGAALV VSEDLVKQTL QDIEQHQEEA CVIGRVVACP KGSPRVKVEH 790 800 810 820 830 840 LIETMQINGS VLENGTLRNH FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV 850 860 870 880 890 900 ISNKAAVAGL DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS 910 920 930 940 950 960 GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV DAGQIILQEA 970 980 990 1000 1010 VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE NGRICWVTED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2." Woehlke A., Droegemueller C., Kuiper H., Leeb T., Distl O. Gene 348:73-81(2005) [PubMed: 15777723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-771.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ780930 Genomic DNA. Translation: CAG47113.1. BC122573 mRNA. Translation: AAI22574.1.
IPI	IPI00709162.
RefSeq	NP_001035563.1.
UniGene	Bt.11270 Bt.94801
3D structure databases
HSSP	HSSP built from PDB template 1ZLY based on UniProtKB P22102.
SMR	Q59A32. Positions 1-430, 446-769, 475-792, 808-1007.
ModBase	Search...
Protein-protein interaction databases
STRING	Q59A32.
Genome annotation databases
Ensembl	ENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188; Bos taurus. [Genome view]
GeneID	281183.
KEGG	bta:281183.
Organism-specific databases
CTD	281183.
Phylogenomic databases
HOVERGEN	Q59A32.
InParanoid	Q59A32.
PhylomeDB	Q59A32.
Enzyme and pathway databases
BRENDA	2.1.2.2. 251. 6.3.3.1. 251. 6.3.4.13. 251.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR004607. PurN_trans. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit. TIGR00878. purM. 1 hit. TIGR00639. PurN. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_BOVIN

Accession

Primary (citable) accession number: Q59A32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	April 26, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)