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Q59A32

- PUR2_BOVIN

UniProt

Q59A32 - PUR2_BOVIN

Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
    10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi288 – 2881ManganeseBy similarity
    Metal bindingi290 – 2901ManganeseBy similarity
    Binding sitei871 – 871110-formyltetrahydrofolateBy similarity
    Binding sitei913 – 913110-formyltetrahydrofolateBy similarity
    Active sitei915 – 9151Proton donorBy similarity
    Sitei951 – 9511Raises pKa of active site HisBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi137 – 19963ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. methyltransferase activity Source: InterPro
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
    5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
    6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_226478. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00126.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
    Including the following 3 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name:
    GART
    Gene namesi
    Name:GART
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 10101009Trifunctional purine biosynthetic protein adenosine-3PRO_0000250715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei350 – 3501N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ59A32.
    PRIDEiQ59A32.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000012108.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59A32.
    SMRiQ59A32. Positions 1-430, 475-792, 808-1007.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini111 – 318208ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni434 – 809376AIRSAdd
    BLAST
    Regioni810 – 1010201GARTAdd
    BLAST
    Regioni818 – 82035'-phosphoribosylglycinamide bindingBy similarity
    Regioni896 – 899410-formyltetrahydrofolate bindingBy similarity
    Regioni947 – 951510-formyltetrahydrofolate bindingBy similarity
    Regioni977 – 98045'-phosphoribosylglycinamide bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the central section; belongs to the AIR synthase family.Curated
    In the C-terminal section; belongs to the GART family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG0151.
    GeneTreeiENSGT00390000000292.
    HOGENOMiHOG000030315.
    HOVERGENiHBG008333.
    InParanoidiQ59A32.
    KOiK11787.
    OMAiGMLFTGF.
    OrthoDBiEOG7RBZ7H.
    TreeFamiTF106368.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    TIGR00639. PurN. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q59A32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI     50
    SISDHTALAQ FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA 100
    AQLESSKRFA KEFMDRHGIS TARWRAFTKP KEACDFIMSA DFPALVVKAS 150
    GLAAGKGVIV AKSKEEACEA VREIMQGKAF GEAGETVVIE ELLEGEEVSC 200
    LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP QVSKDLLLKI 250
    KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV 300
    ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK 350
    GVEITGFPEA QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL 400
    EEARKGLAAI KFEGAVYRKD IGFRAIAFLQ QPRGLTYKES GVDIAAGNML 450
    VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK AAGFTDPLLA CGTDGVGTKL 500
    KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC GKLDLRTTEA 550
    VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP 600
    QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT 650
    LGDLLLTPTK IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV 700
    NLDAQTWRVP RIFSWLQQEG HLSEEEMART FNCGIGAALV VSEDLVKQTL 750
    QDIEQHQEEA CVIGRVVACP KGSPRVKVEH LIETMQINGS VLENGTLRNH 800
    FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV ISNKAAVAGL 850
    DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS 900
    GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV 950
    DAGQIILQEA VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE 1000
    NGRICWVTED 1010
    Length:1,010
    Mass (Da):107,907
    Last modified:April 26, 2005 - v1
    Checksum:i27DFCA438661A771
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti771 – 7711K → E.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ780930 Genomic DNA. Translation: CAG47113.1.
    BC122573 mRNA. Translation: AAI22574.1.
    RefSeqiNP_001035563.1. NM_001040473.2.
    XP_005201140.1. XM_005201083.1.
    XP_005201141.1. XM_005201084.1.
    UniGeneiBt.11270.
    Bt.94801.

    Genome annotation databases

    EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188.
    GeneIDi281183.
    KEGGibta:281183.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ780930 Genomic DNA. Translation: CAG47113.1 .
    BC122573 mRNA. Translation: AAI22574.1 .
    RefSeqi NP_001035563.1. NM_001040473.2.
    XP_005201140.1. XM_005201083.1.
    XP_005201141.1. XM_005201084.1.
    UniGenei Bt.11270.
    Bt.94801.

    3D structure databases

    ProteinModelPortali Q59A32.
    SMRi Q59A32. Positions 1-430, 475-792, 808-1007.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000012108.

    Proteomic databases

    PaxDbi Q59A32.
    PRIDEi Q59A32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012108 ; ENSBTAP00000012108 ; ENSBTAG00000009188 .
    GeneIDi 281183.
    KEGGi bta:281183.

    Organism-specific databases

    CTDi 2618.

    Phylogenomic databases

    eggNOGi COG0151.
    GeneTreei ENSGT00390000000292.
    HOGENOMi HOG000030315.
    HOVERGENi HBG008333.
    InParanoidi Q59A32.
    KOi K11787.
    OMAi GMLFTGF.
    OrthoDBi EOG7RBZ7H.
    TreeFami TF106368.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00126 .
    UPA00074 ; UER00129 .
    Reactomei REACT_226478. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    NextBioi 20805240.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    TIGR00639. PurN. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2."
      Woehlke A., Droegemueller C., Kuiper H., Leeb T., Distl O.
      Gene 348:73-81(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-771.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Uterus.

    Entry informationi

    Entry nameiPUR2_BOVIN
    AccessioniPrimary (citable) accession number: Q59A32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3