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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (PPAT), Amidophosphoribosyltransferase (PPAT)
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288ManganeseBy similarity1
Metal bindingi290ManganeseBy similarity1
Binding sitei87110-formyltetrahydrofolateBy similarity1
Binding sitei91310-formyltetrahydrofolateBy similarity1
Active sitei915Proton donorBy similarity1
Sitei951Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 199ATPBy similarityAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00125
UPA00074; UER00126
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Organism-specific databases

VGNCiVGNC:29255 GART

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002507152 – 1010Trifunctional purine biosynthetic protein adenosine-3Add BLAST1009

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei350N6-acetyllysineBy similarity1
Modified residuei440PhosphoserineBy similarity1
Modified residuei682PhosphothreonineBy similarity1
Modified residuei802PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ59A32
PeptideAtlasiQ59A32
PRIDEiQ59A32

Expressioni

Gene expression databases

BgeeiENSBTAG00000009188

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012108

Structurei

3D structure databases

ProteinModelPortaliQ59A32
SMRiQ59A32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 318ATP-graspAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni434 – 809AIRSAdd BLAST376
Regioni810 – 1010GARTAdd BLAST201
Regioni818 – 8205'-phosphoribosylglycinamide bindingBy similarity3
Regioni896 – 89910-formyltetrahydrofolate bindingBy similarity4
Regioni947 – 95110-formyltetrahydrofolate bindingBy similarity5
Regioni977 – 9805'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated

Phylogenomic databases

eggNOGiKOG0237 Eukaryota
KOG3076 Eukaryota
COG0150 LUCA
COG0151 LUCA
COG0299 LUCA
GeneTreeiENSGT00390000000292
HOGENOMiHOG000030315
HOVERGENiHBG008333
InParanoidiQ59A32
KOiK11787
OMAiLLERHNC
OrthoDBiEOG091G026D
TreeFamiTF106368

Family and domain databases

CDDicd08645 FMT_core_GART, 1 hit
cd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
MF_01930 PurN, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004607 GART
IPR001555 GART_AS
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF00551 Formyl_trans_N, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF53328 SSF53328, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
TIGR00639 PurN, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit
PS00373 GART, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI
60 70 80 90 100
SISDHTALAQ FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAQA
110 120 130 140 150
AQLESSKRFA KEFMDRHGIS TARWRAFTKP KEACDFIMSA DFPALVVKAS
160 170 180 190 200
GLAAGKGVIV AKSKEEACEA VREIMQGKAF GEAGETVVIE ELLEGEEVSC
210 220 230 240 250
LCFTDGRTVA PMPPAQDHKR LLEGDEGPNT GGMGAYCPAP QVSKDLLLKI
260 270 280 290 300
KNNILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVIQSILDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK
360 370 380 390 400
GVEITGFPEA QALGLEVFQA GTALKDGKVV TNGGRVLTVT AIRENLISAL
410 420 430 440 450
EEARKGLAAI KFEGAVYRKD IGFRAIAFLQ QPRGLTYKES GVDIAAGNML
460 470 480 490 500
VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK AAGFTDPLLA CGTDGVGTKL
510 520 530 540 550
KIAQQCSKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFSC GKLDLRTTEA
560 570 580 590 600
VITGIAKACK KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP
610 620 630 640 650
QLERITEGDA VIGIASSGLH SNGFSLVRKI VAKSSLEYSS PAPGGCGDQT
660 670 680 690 700
LGDLLLTPTK IYSRSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV
710 720 730 740 750
NLDAQTWRVP RIFSWLQQEG HLSEEEMART FNCGIGAALV VSEDLVKQTL
760 770 780 790 800
QDIEQHQEEA CVIGRVVACP KGSPRVKVEH LIETMQINGS VLENGTLRNH
810 820 830 840 850
FSVQPKKARV AVLISGTGSN LQALIDSTRE PSSLAHIVIV ISNKAAVAGL
860 870 880 890 900
DKAEKAGIPT RVINHKLYKN RAAFDTAIDE VLEEFSTDIV CLAGFMRILS
910 920 930 940 950
GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTVTG CTVHFVAEDV
960 970 980 990 1000
DAGQIILQEA VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVRLGE
1010
NGRICWVTED
Length:1,010
Mass (Da):107,907
Last modified:April 26, 2005 - v1
Checksum:i27DFCA438661A771
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti771K → E1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ780930 Genomic DNA Translation: CAG47113.1
BC122573 mRNA Translation: AAI22574.1
RefSeqiNP_001035563.1, NM_001040473.2
UniGeneiBt.11270
Bt.94801

Genome annotation databases

EnsembliENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188
GeneIDi281183
KEGGibta:281183

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPUR2_BOVIN
AccessioniPrimary (citable) accession number: Q59A32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: April 26, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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