Reviewed,
UniProtKB/Swiss-Prot Q59A32 (PUR2_BOVIN)
Last modified
February 9, 2010.
Version 42.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Trifunctional purine biosynthetic protein adenosine-3 Including the following 3 domains: 1- Recommended name: Phosphoribosylamine--glycine ligase EC=6.3.4.13 Alternative name(s): Glycinamide ribonucleotide synthetase Short name=GARS Phosphoribosylglycinamide synthetase 2- Recommended name: Phosphoribosylformylglycinamidine cyclo-ligase EC=6.3.3.1 Alternative name(s): Phosphoribosyl-aminoimidazole synthetase AIR synthase Short name=AIRS 3- Recommended name: Phosphoribosylglycinamide formyltransferase EC=2.1.2.2 Alternative name(s): 5'-phosphoribosylglycinamide transformylase GAR transformylase Short name=GART | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 1010 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. |
| Pathway | |
| Sequence similarities | In the N-terminal section; belongs to the GARS family. In the central section; belongs to the AIR synthase family. In the C-terminal section; belongs to the GART family. Contains 1 ATP-grasp domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 1010 | 1009 | Trifunctional purine biosynthetic protein adenosine-3 | PRO_0000250715 | |||||
Regions | |||||||||
| Domain | 111 – 318 | 208 | ATP-grasp | ||||||
| Nucleotide binding | 137 – 199 | 63 | ATP By similarity | ||||||
| Region | 434 – 809 | 376 | AIRS | ||||||
| Region | 810 – 1010 | 201 | GART | ||||||
| Region | 818 – 820 | 3 | 5'-phosphoribosylglycinamide binding By similarity | ||||||
| Region | 896 – 899 | 4 | 10-formyltetrahydrofolate binding By similarity | ||||||
| Region | 947 – 951 | 5 | 10-formyltetrahydrofolate binding By similarity | ||||||
| Region | 977 – 980 | 4 | 5'-phosphoribosylglycinamide binding By similarity | ||||||
Sites | |||||||||
| Active site | 915 | 1 | Proton donor By similarity | ||||||
| Metal binding | 288 | 1 | Manganese By similarity | ||||||
| Metal binding | 290 | 1 | Manganese By similarity | ||||||
| Binding site | 871 | 1 | 10-formyltetrahydrofolate By similarity | ||||||
| Binding site | 913 | 1 | 10-formyltetrahydrofolate By similarity | ||||||
| Site | 951 | 1 | Raises pKa of active site His By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine By similarity | ||||||
| Modified residue | 348 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 350 | 1 | N6-acetyllysine By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 771 | 1 | K → E | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2." Woehlke A., Droegemueller C., Kuiper H., Leeb T., Distl O. Gene 348:73-81(2005) [PubMed: 15777723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-771. |
| [2] | NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ780930 Genomic DNA. Translation: CAG47113.1. BC122573 mRNA. Translation: AAI22574.1. |
| IPI | IPI00709162. |
| RefSeq | NP_001035563.1. |
| UniGene | Bt.11270 Bt.94801 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ZLY based on UniProtKB P22102. |
| SMR | Q59A32. Positions 1-430, 446-769, 475-792, 808-1007. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q59A32. |
Genome annotation databases | |
| Ensembl | ENSBTAT00000012108; ENSBTAP00000012108; ENSBTAG00000009188; Bos taurus. [Genome view] |
| GeneID | 281183. |
| KEGG | bta:281183. |
Organism-specific databases | |
| CTD | 281183. |
Phylogenomic databases | |
| HOVERGEN | Q59A32. |
| InParanoid | Q59A32. |
| PhylomeDB | Q59A32. |
Enzyme and pathway databases | |
| BRENDA | 2.1.2.2. 251. 6.3.3.1. 251. 6.3.4.13. 251. |
Family and domain databases | |
| InterPro | IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR004607. PurN_trans. IPR011054. Rudment_hybrid_motif. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00877. purD. 1 hit. TIGR00878. purM. 1 hit. TIGR00639. PurN. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. PS00373. GART. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR2_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q59A32 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


