Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q599T9

- MYD88_BOVIN

UniProt

Q599T9 - MYD88_BOVIN

Protein

Myeloid differentiation primary response protein MyD88

Gene

MYD88

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.2 Publications

    GO - Biological processi

    1. defense response to Gram-positive bacterium Source: UniProtKB
    2. immunoglobulin mediated immune response Source: Ensembl
    3. inflammatory response Source: UniProtKB-KW
    4. innate immune response Source: UniProtKB-KW
    5. lipopolysaccharide-mediated signaling pathway Source: Ensembl
    6. MyD88-dependent toll-like receptor signaling pathway Source: Ensembl
    7. negative regulation of growth of symbiont in host Source: Ensembl
    8. positive regulation of chemokine biosynthetic process Source: Ensembl
    9. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    10. positive regulation of interleukin-17 production Source: Ensembl
    11. positive regulation of interleukin-23 production Source: Ensembl
    12. positive regulation of interleukin-6 production Source: Ensembl
    13. positive regulation of JNK cascade Source: Ensembl
    14. positive regulation of lymphocyte proliferation Source: Ensembl
    15. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
    16. positive regulation of tumor necrosis factor production Source: Ensembl
    17. regulation of inflammatory response Source: Ensembl
    18. response to molecule of fungal origin Source: Ensembl
    19. response to peptidoglycan Source: Ensembl
    20. response to virus Source: Ensembl
    21. type I interferon biosynthetic process Source: Ensembl

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Enzyme and pathway databases

    ReactomeiREACT_207058. TRAF6 mediated NF-kB activation.
    REACT_207826. Interleukin-1 signaling.
    REACT_210227. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
    REACT_215391. RIP-mediated NFkB activation via ZBP1.
    REACT_217214. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_218091. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
    REACT_218986. TRAF6 mediated IRF7 activation.
    REACT_219586. MyD88 dependent cascade initiated on endosome.
    REACT_227494. p75NTR recruits signalling complexes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myeloid differentiation primary response protein MyD88
    Gene namesi
    Name:MYD88
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 22

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Myeloid differentiation primary response protein MyD88PRO_0000393129Add
    BLAST

    Proteomic databases

    PRIDEiQ599T9.

    Interactioni

    Subunit structurei

    Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1, IKBKE and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Upon IL1B treatment, forms a complex with PELI1, IRAK1, IRAK4 and TRAF6; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. May interact with PIK3AP1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000000735.

    Structurei

    3D structure databases

    ProteinModelPortaliQ599T9.
    SMRiQ599T9. Positions 146-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 10978DeathPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 296138TIRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 15546Intermediate domainBy similarityAdd
    BLAST

    Domaini

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.By similarity

    Sequence similaritiesi

    Contains 1 death domain.PROSITE-ProRule annotation
    Contains 1 TIR domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG70664.
    GeneTreeiENSGT00510000048324.
    HOGENOMiHOG000068971.
    HOVERGENiHBG052547.
    InParanoidiQ599T9.
    KOiK04729.
    OMAiRCKRMVV.
    OrthoDBiEOG7H792K.
    TreeFamiTF326264.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    3.40.50.10140. 1 hit.
    InterProiIPR011029. DEATH-like_dom.
    IPR000488. Death_domain.
    IPR017281. Myelin_different_resp_MyD88.
    IPR000157. TIR_dom.
    [Graphical view]
    PfamiPF00531. Death. 1 hit.
    PF01582. TIR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037756. MyD88. 1 hit.
    SMARTiSM00005. DEATH. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    SSF52200. SSF52200. 1 hit.
    PROSITEiPS50017. DEATH_DOMAIN. 1 hit.
    PS50104. TIR. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q599T9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEGVPRAGS ALPAASLSSL PLAALNVRVR RRLSLFLNVR APVAADWTVL    50
    AEAMDFEYLE IQQLEKYADP TSRLLDDWQR RPGASVGRLL ELLAKLGRDD 100
    VLMELGPSIE EDCQKYILKQ QQEASEKPLQ VDSIDSSITR INDMAGITIR 150
    DDPLGQKPEC FDAFICYCPS DIEFVHEMIR QLEQTNYRLK LCVSDRDVLP 200
    GTCVWSIASE LIEKRCRRMV VVVSDEYLQS KECDFQTKFA LSLSPGAHQK 250
    RLIPIKYKPM KKEFPSILRF ITVCDYTNPC TQNWFWTRLA KALSMP 296
    Length:296
    Mass (Da):33,709
    Last modified:April 26, 2005 - v1
    Checksum:iCA644B4AE7B1D9AA
    GO
    Isoform 2 (identifier: Q599T9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         111-155: Missing.

    Show »
    Length:251
    Mass (Da):28,663
    Checksum:i1398D566C39E81F8
    GO
    Isoform 3 (identifier: Q599T9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         111-155: Missing.
         216-264: CRRMVVVVSD...IKYKPMKKEF → LATWPQGKWV...LSPGGSPRCL
         265-296: Missing.

    Show »
    Length:219
    Mass (Da):24,228
    Checksum:i3ED4B61A6B28242B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei111 – 15545Missing in isoform 2 and isoform 3. 1 PublicationVSP_038889Add
    BLAST
    Alternative sequencei216 – 26449CRRMV…MKKEF → LATWPQGKWVGVQSPARDPH AGVLPAGPCLAWHSGILPRL SPGGSPRCL in isoform 3. 1 PublicationVSP_038890Add
    BLAST
    Alternative sequencei265 – 29632Missing in isoform 3. 1 PublicationVSP_038891Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ853453 mRNA. Translation: CAH68521.1.
    AY934808 mRNA. Translation: AAY16578.1.
    AY937381 mRNA. Translation: AAY22119.1.
    BC102851 mRNA. Translation: AAI02852.1.
    AY634627 mRNA. Translation: AAT48485.1.
    RefSeqiNP_001014404.1. NM_001014382.2. [Q599T9-1]
    UniGeneiBt.48995.

    Genome annotation databases

    EnsembliENSBTAT00000000735; ENSBTAP00000000735; ENSBTAG00000000563. [Q599T9-1]
    GeneIDi444881.
    KEGGibta:444881.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ853453 mRNA. Translation: CAH68521.1 .
    AY934808 mRNA. Translation: AAY16578.1 .
    AY937381 mRNA. Translation: AAY22119.1 .
    BC102851 mRNA. Translation: AAI02852.1 .
    AY634627 mRNA. Translation: AAT48485.1 .
    RefSeqi NP_001014404.1. NM_001014382.2. [Q599T9-1 ]
    UniGenei Bt.48995.

    3D structure databases

    ProteinModelPortali Q599T9.
    SMRi Q599T9. Positions 146-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000000735.

    Proteomic databases

    PRIDEi Q599T9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000000735 ; ENSBTAP00000000735 ; ENSBTAG00000000563 . [Q599T9-1 ]
    GeneIDi 444881.
    KEGGi bta:444881.

    Organism-specific databases

    CTDi 4615.

    Phylogenomic databases

    eggNOGi NOG70664.
    GeneTreei ENSGT00510000048324.
    HOGENOMi HOG000068971.
    HOVERGENi HBG052547.
    InParanoidi Q599T9.
    KOi K04729.
    OMAi RCKRMVV.
    OrthoDBi EOG7H792K.
    TreeFami TF326264.

    Enzyme and pathway databases

    Reactomei REACT_207058. TRAF6 mediated NF-kB activation.
    REACT_207826. Interleukin-1 signaling.
    REACT_210227. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
    REACT_215391. RIP-mediated NFkB activation via ZBP1.
    REACT_217214. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_218091. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
    REACT_218986. TRAF6 mediated IRF7 activation.
    REACT_219586. MyD88 dependent cascade initiated on endosome.
    REACT_227494. p75NTR recruits signalling complexes.

    Miscellaneous databases

    NextBioi 20831769.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    3.40.50.10140. 1 hit.
    InterProi IPR011029. DEATH-like_dom.
    IPR000488. Death_domain.
    IPR017281. Myelin_different_resp_MyD88.
    IPR000157. TIR_dom.
    [Graphical view ]
    Pfami PF00531. Death. 1 hit.
    PF01582. TIR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037756. MyD88. 1 hit.
    SMARTi SM00005. DEATH. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    SSF52200. SSF52200. 1 hit.
    PROSITEi PS50017. DEATH_DOMAIN. 1 hit.
    PS50104. TIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 (CXCL8) expression in the udder."
      Yang W., Zerbe H., Petzl W., Brunner R.M., Gunther J., Draing C., von Aulock S., Schuberth H.J., Seyfert H.M.
      Mol. Immunol. 45:1385-1397(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
      Tissue: Mammary gland.
    2. "Regulation of TLR-mediated NFkB activation by alternative splicing of bovine MyD88."
      Bailey R., Yamakawa Y., Stalker A., Willcocks S., Werling D.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    4. "Expression of TOLL-like receptors (TLR) by bovine antigen-presenting cells-potential role in pathogen discrimination?"
      Werling D., Piercy J., Coffey T.J.
      Vet. Immunol. Immunopathol. 112:2-11(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 206-296 (ISOFORMS 1/2).
    5. "Functional characterization of bovine TIRAP and MyD88 in mediating bacterial lipopolysaccharide-induced endothelial NF-kappaB activation and apoptosis."
      Cates E.A., Connor E.E., Mosser D.M., Bannerman D.D.
      Comp. Immunol. Microbiol. Infect. Dis. 32:477-490(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMYD88_BOVIN
    AccessioniPrimary (citable) accession number: Q599T9
    Secondary accession number(s): Q49BZ6, Q49CF6, Q6GV22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3