Reviewed, UniProtKB/Swiss-Prot Q59978 (GRPE_SYNY3)

Names and origin

Protein names

Recommended name:
    Protein grpE
Alternative name(s):
    HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	sll0057

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the grpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro protein import into mitochondrial matrix Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: InterPro
Molecular function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro chaperone binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Protein grpE HAMAP MF_01151		PRO_0000113880

Sequences

	Sequence		Length	Mass (Da)
	Q59978-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C69CB5D0EB39B248	FASTA	249	27,568
	10 20 30 40 50 60 MNEDQVSLEN QTNPVSPEAS DVPAVTPEES PQPTDAVLGE PSGEQSEDPR IGAATETEGG 70 80 90 100 110 120 PLEQEKSSEE IIAILQKDLA SHRQELAEQS EQLDSIKKRY VALAAEFDNF RKRTQREKEE 130 140 150 160 170 180 QAKLIKGRTI TELLPVVDNF ERARTQIKPN SDGENQIHKS YQGVYKNLVD SLKGLGVAPM 190 200 210 220 230 240 RPEGKPFDPK YHEAMLREPT AEYPEDTVIE ELVRGYLLDD IVLRHSMVKV AVAPEEGAEV VNGEAGANP

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10291.1.
PIR	S74373.
RefSeq	NP_442221.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q59978. 8 interactions.
STRING	Q59978.
Genome annotation databases
GeneID	952574.
GenomeReviews	Gene locus sll0057 in contig BA000022_GR.
KEGG	syn:sll0057.
NMPDR	fig|1148.1.peg.2322.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732630.
OMA	ELEGESC.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0057-MON.
Family and domain databases
HAMAP	MF_01151. [Tree]
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
Gene3D	G3DSA:3.90.20.20. GrpE_coiled_coil. 1 hit. G3DSA:2.30.22.10. GrpE_head. 1 hit.
PANTHER	PTHR21237. GrpE. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_SYNY3

Accession

Primary (citable) accession number: Q59978

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)