Q59975 (AROA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	slr0444

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088311

Sequences

	Sequence		Length	Mass (Da)
	Q59975 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2F3C8AD26B5A7BCE	FASTA	447	47,047
	10 20 30 40 50 60 MALLSLNNHQ SHQRLTVNPP AQGVALTGRL RVPGDKSISH RALMLGAIAT GETIIEGLLL 70 80 90 100 110 120 GEDPRSTAHC FRAMGAEISE LNSEKIIVQG RGLGQLQEPS TVLDAGNSGT TMRLMLGLLA 130 140 150 160 170 180 GQKDCLFTVT GDDSLRHRPM SRVIQPLQQM GAKIWARSNG KFAPLAVQGS QLKPIHYHSP 190 200 210 220 230 240 IASAQVKSCL LLAGLTTEGD TTVTEPALSR DHSERMLQAF GAKLTIDPVT HSVTVHGPAH 250 260 270 280 290 300 LTGQRVVVPG DISSAAFWLV AASILPGSEL LVENVGINPT RTGVLEVLAQ MGADITPENE 310 320 330 340 350 360 RLVTGEPVAD LRVRASHLQG CTFGGEIIPR LIDEIPILAV AAAFAEGTTR IEDAAELRVK 370 380 390 400 410 420 ESDRLAAIAS ELGKMGAKVT EFDDGLEIQG GSPLQGAEVD SLTDHRIAMA LAIAALGSGG 430 440 QTIINRAEAA AISYPEFFGT LGQVAQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An aroA homologue from Synechocystis sp. PCC 6803." dalla Chiesa M., Mayes S.R., Maskell D.S., Nixon P.J., Barber J. Gene 144:145-146(1994) [PubMed: 8026753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[3]	"The genes aroA and trnQ are located upstream of psbO in the chromosome of Synechocystis 6803." Mayes S.R., dalla Chiesa M., Zhang Z., Barber J. FEBS Lett. 325:255-261(1993) [PubMed: 7686511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-194.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75325 Genomic DNA. Translation: CAA53074.1. BA000022 Genomic DNA. Translation: BAA18477.1. X72784 Genomic DNA. Translation: CAA51291.1.
PIR	S76218.
RefSeq	NP_441799.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q59975.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q59975. 1 interaction.
STRING	Q59975.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952914.
GenomeReviews	Gene locus slr0444 in contig BA000022_GR.
KEGG	syn:slr0444.
NMPDR	fig|1148.1.peg.1900.
PATRIC	23841098. VBISynSp132158_2101.
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	GEDPRST.
PhylomeDB	Q59975.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0444-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_SYNY3

Accession

Primary (citable) accession number: Q59975
Secondary accession number(s): Q59974

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program