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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei258NucleophileBy similarity1
Active sitei343By similarity1
Active sitei345By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Protein family/group databases

MEROPSiC26.A33.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Ordered Locus Names:SSO0640
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123651 – 367Carbamoyl-phosphate synthase small chainAdd BLAST367

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

STRINGi273057.SSO0640.

Structurei

3D structure databases

ProteinModelPortaliQ59968.
SMRiQ59968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini182 – 367Glutamine amidotransferase type-1Add BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 182CPSaseAdd BLAST182

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00064. Archaea.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiQ59968.
KOiK01956.
OMAiCFNTGMT.
OrthoDBiPOG093Z085N.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR036480. CarbP_synth_ssu_N_sf.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLENKKGYL YLEDGTFIEG YSFGAKGIKV GEVVFTTSMN GYVESLTDPS
60 70 80 90 100
YKGQILIITH PLVGNYGVPE KKYEQGILTN FESERIQVEG LIVAEHTYPS
110 120 130 140 150
KWNSALTLDE WLKSENVPGV FDVDTRMIVK KIRTYGTMMG IIASELEIDD
160 170 180 190 200
PRKYLEKKYD EIDFTQFTSP KSPIFHPNTG DMIVVVDCGI KHGILYGLYK
210 220 230 240 250
RGFSIVRVPC SFSASKIIEY NPKGIVFSNG PGNPNLLENQ IKTFSELVEY
260 270 280 290 300
KIPILGICLG HQIATLALGG KIKKMKFGHR AINKPVIESN SNKCYISTHN
310 320 330 340 350
HGYGIISKND IPPNTKIWFY NPDDYTIEGL IHEKLPIITT QFHPEARPGP
360
WDTTWVFDKF RTMVTGK
Length:367
Mass (Da):41,480
Last modified:November 1, 1996 - v1
Checksum:i814FF3E5D2526C80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33768 Genomic DNA. Translation: AAA99058.1.
Y18930 Genomic DNA. Translation: CAB57661.1.
AE006641 Genomic DNA. Translation: AAK40948.1.
PIRiT43252.
RefSeqiWP_009991178.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40948; AAK40948; SSO0640.
GeneIDi27426942.
KEGGisso:SSO0640.
PATRICifig|273057.12.peg.645.

Similar proteinsi

Entry informationi

Entry nameiCARA_SULSO
AccessioniPrimary (citable) accession number: Q59968
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families