Reviewed, UniProtKB/Swiss-Prot Q59966 (SRPG_SYNE7)

Names and origin

Protein names

Recommended name:
    Cysteine synthase, plasmid
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	srpG
Ordered Locus Names:	Synpcc7942_B2664
ORF Names:	pANL40

Encoded on

Plasmid pANL

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Cysteine synthase, plasmid		PRO_0000167115
Regions
	Region	183 – 187	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	78	1	Pyridoxal phosphate By similarity
	Binding site	278	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	48	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59966-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3800262555CD7745	FASTA	329	34,601
	10 20 30 40 50 60 MSTSGTFFAD NSQTIGKTPL VRLNRIVKGA PATVLAKIEG RNPAYSVKCR IGAAMIWDAE 70 80 90 100 110 120 QRGLLGPGKE LIEPTSGNTG IALAFVAAAR GIPLTLTMPE TMSLERRKLL AAYGAKLVLT 130 140 150 160 170 180 EGVKGMTGAV RRAEDIAASD PDRYVLLQQF RNPANPAIHE QTTGPEIWED TGGAIDILVS 190 200 210 220 230 240 GVGTGGTITG VSRYIKQTQG KPILSVAVEP EASPVISQQR SGLPLKPGPH KIQGIGAGFI 250 260 270 280 290 300 PENLDLSLVD QVERVSNEEA IAYARRLAQE EGLISGISCG AAVAAAVRLA QQSEHAGKTI 310 320 VVVLPDSGER YLSTALFDGI FNEQGLAVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two enzymes together capable of cysteine biosynthesis are encoded on a cyanobacterial plasmid." Nicholson M.L., Gaasenbeek M., Laudenbach D.E. Mol. Gen. Genet. 247:623-632(1995) [PubMed: 7603442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"pANL, the large plasmid of Synechococcus elongatus PCC 7942." Holtman C.K., Chen Y., Sandoval P., Socias T., Mohler B.J., Youderian P., Golden S.S. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of plasmid 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23436 Genomic DNA. Translation: AAA86725.1. AF441790 Genomic DNA. Translation: AAM81167.1. CP000101 Genomic DNA. Translation: ABB58693.1.
RefSeq	NP_665779.1. YP_398713.1.
3D structure databases
SMR	Q59966. Positions 8-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q59966.
Genome annotation databases
GeneID	1010237. 3772773.
GenomeReviews	Gene locus Synpcc7942_B2664 in contig CP000101_GR.
KEGG	syf:Synpcc7942_B2664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0031.
HOGENOM	HBG748215.
Enzyme and pathway databases
BioCyc	SELO1140:PANL40-MONOMER. SELO1140:SYNPCC7942_B2664-MONOMER.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRPG_SYNE7

Accession

Primary (citable) accession number: Q59966
Secondary accession number(s): Q7BA84

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)