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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation2 Publications

Cofactori

Mg2+UniRule annotation1 Publication, Mn2+UniRule annotation1 Publication, Ca2+UniRule annotation1 PublicationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation1 Publication

Enzyme regulationi

Inhibited by quinolones, such as levofloxacin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATPUniRule annotation1
Binding sitei51ATPUniRule annotation1
Binding sitei78ATPUniRule annotation1
Binding sitei344ATPUniRule annotation1
Metal bindingi433Magnesium 1; catalyticUniRule annotation1 Publication1
Metal bindingi506Magnesium 1; catalyticUniRule annotation1 Publication1
Metal bindingi506Magnesium 2UniRule annotation1 Publication1
Metal bindingi508Magnesium 2UniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi118 – 124ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:SP_0852
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2363033.
DrugBankiDB01044. Gatifloxacin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454431 – 647DNA topoisomerase 4 subunit BAdd BLAST647

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei458Interaction with DNAUniRule annotation1
Sitei461Interaction with DNAUniRule annotation1
Sitei513Interaction with DNAUniRule annotation1
Sitei629Interaction with DNAUniRule annotation1

Protein-protein interaction databases

DIPiDIP-48520N.
STRINGi170187.SpneT_02001165.

Structurei

Secondary structure

1647
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni424 – 426Combined sources3
Beta strandi428 – 433Combined sources6
Helixi435 – 443Combined sources9
Turni447 – 449Combined sources3
Beta strandi450 – 455Combined sources6
Turni462 – 464Combined sources3
Helixi467 – 471Combined sources5
Helixi474 – 483Combined sources10
Helixi488 – 490Combined sources3
Helixi493 – 495Combined sources3
Beta strandi499 – 504Combined sources6
Helixi509 – 524Combined sources16
Helixi527 – 531Combined sources5
Beta strandi535 – 538Combined sources4
Beta strandi542 – 547Combined sources6
Helixi550 – 552Combined sources3
Beta strandi554 – 558Combined sources5
Helixi561 – 570Combined sources10
Beta strandi576 – 579Combined sources4
Helixi583 – 585Combined sources3
Helixi588 – 595Combined sources8
Turni598 – 600Combined sources3
Beta strandi603 – 607Combined sources5
Helixi612 – 622Combined sources11
Helixi627 – 636Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FOEX-ray4.00C/D404-647[»]
3FOFX-ray4.00C/D404-647[»]
3K9FX-ray2.90C/D404-647[»]
3KSAX-ray3.30C/D404-647[»]
3KSBX-ray3.50C/D404-647[»]
3LTNX-ray3.10C/D404-647[»]
3RADX-ray3.35C/D404-647[»]
3RAEX-ray2.90C/D404-647[»]
3RAFX-ray3.24C/D404-647[»]
4JUOX-ray6.53C1-647[»]
4KOEX-ray3.02C/D404-647[»]
4KPEX-ray3.43C/D404-647[»]
4KPFX-ray3.24C/D404-647[»]
4Z3OX-ray3.44A/B404-643[»]
4Z4QX-ray3.04A/B404-643[»]
4Z53X-ray3.26A/B404-643[»]
ProteinModelPortaliQ59961.
SMRiQ59961.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59961.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini427 – 541ToprimUniRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 2 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
HOGENOMiHOG000075154.
KOiK02622.
OMAiKFRMGED.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKEININN YNDDAIQVLE GLDAVRKRPG MYIGSTDGAG LHHLVWEIVD
60 70 80 90 100
NAVDEALSGF GDRIDVTINK DGSLTVQDHG RGMPTGMHAM GIPTVEVIFT
110 120 130 140 150
ILHAGGKFGQ GGYKTSGGLH GVGSSVVNAL SSWLEVEITR DGAVYKQRFE
160 170 180 190 200
NGGKPVTTLK KIGTAPKSKT GTKVTFMPDA TIFSTTDFKY NTISERLNES
210 220 230 240 250
AFLLKNVTLS LTDKRTNEAI EFHYENGVQD FVSYLNEDKE ILTPVLYFEG
260 270 280 290 300
EDNGFQVEVA LQYNDGFSDN ILSFVNNVRT KDGGTHETGL KSAITKVMND
310 320 330 340 350
YARKTGLLKE KDKNLEGSDY REGLAAVLSI LVPEEHLQFE GQTKDKLGSP
360 370 380 390 400
LARPVVDGIV ADKLTFFLME NGELASNLIR KAIKARDARE AARKARDESR
410 420 430 440 450
NGKKNKKDKG LLSGKLTPAQ SKNPAKNELY LVEGDSAGGS AKQGRDRKFQ
460 470 480 490 500
AILPLRGKVV NTAKAKMADI LKNEEINTMI YTIGAGVGAD FSIEDANYDK
510 520 530 540 550
IIIMTDADTD GAHIQTLLLT FFYRYMRPLV EAGHVYIALP PLYKMSKGKG
560 570 580 590 600
KKEEVAYAWT DGELEELRKQ FGKGATLQRY KGLGEMNADQ LWETTMNPET
610 620 630 640
RTLIRVTIED LARAERRVNV LMGDKVEPRR KWIEDNVKFT LEETTVF
Length:647
Mass (Da):71,664
Last modified:September 26, 2001 - v3
Checksum:iB688A526C9A4E7BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166P → L in CAA65022 (PubMed:8891124).Curated1
Sequence conflicti217N → D (PubMed:8763932).Curated1
Sequence conflicti217N → D (PubMed:8891124).Curated1
Sequence conflicti460V → I (PubMed:8763932).Curated1
Sequence conflicti460V → I (PubMed:8891124).Curated1
Sequence conflicti568R → L in CAA65022 (PubMed:8891124).Curated1
Sequence conflicti644T → A (PubMed:8763932).Curated1
Sequence conflicti644T → A (PubMed:8891124).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67739 Genomic DNA. Translation: CAA91550.2.
AE005672 Genomic DNA. Translation: AAK74981.1.
X95717 Genomic DNA. Translation: CAA65022.1.
PIRiD95098.
RefSeqiWP_000037283.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK74981; AAK74981; SP_0852.
KEGGispn:SP_0852.
PATRICi19706089. VBIStrPne105772_0891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67739 Genomic DNA. Translation: CAA91550.2.
AE005672 Genomic DNA. Translation: AAK74981.1.
X95717 Genomic DNA. Translation: CAA65022.1.
PIRiD95098.
RefSeqiWP_000037283.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FOEX-ray4.00C/D404-647[»]
3FOFX-ray4.00C/D404-647[»]
3K9FX-ray2.90C/D404-647[»]
3KSAX-ray3.30C/D404-647[»]
3KSBX-ray3.50C/D404-647[»]
3LTNX-ray3.10C/D404-647[»]
3RADX-ray3.35C/D404-647[»]
3RAEX-ray2.90C/D404-647[»]
3RAFX-ray3.24C/D404-647[»]
4JUOX-ray6.53C1-647[»]
4KOEX-ray3.02C/D404-647[»]
4KPEX-ray3.43C/D404-647[»]
4KPFX-ray3.24C/D404-647[»]
4Z3OX-ray3.44A/B404-643[»]
4Z4QX-ray3.04A/B404-643[»]
4Z53X-ray3.26A/B404-643[»]
ProteinModelPortaliQ59961.
SMRiQ59961.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48520N.
STRINGi170187.SpneT_02001165.

Chemistry databases

ChEMBLiCHEMBL2363033.
DrugBankiDB01044. Gatifloxacin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74981; AAK74981; SP_0852.
KEGGispn:SP_0852.
PATRICi19706089. VBIStrPne105772_0891.

Phylogenomic databases

eggNOGiENOG4105C7D. Bacteria.
COG0187. LUCA.
HOGENOMiHOG000075154.
KOiK02622.
OMAiKFRMGED.

Miscellaneous databases

EvolutionaryTraceiQ59961.
PROiQ59961.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE_type2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPARE_STRPN
AccessioniPrimary (citable) accession number: Q59961
Secondary accession number(s): P77956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.