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Q59931

- GAPN_STRMU

UniProt

Q59931 - GAPN_STRMU

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Protein
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene
gapN, SMU_676
Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031Substrate
Binding sitei151 – 1511NADP
Binding sitei177 – 1771NADP
Binding sitei180 – 1801NADP
Binding sitei215 – 2151NADP
Active sitei250 – 2501 By similarity
Active sitei284 – 2841 By similarity
Binding sitei377 – 3771NADP
Binding sitei437 – 4371Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi230 – 25122NADP
Add
BLAST

GO - Molecular functioni

  1. glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13095.
SMUT210007:GC7Z-660-MONOMER.
SABIO-RKQ59931.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.9)
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)]
Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
Triosephosphate dehydrogenase
Gene namesi
Name:gapN
Ordered Locus Names:SMU_676
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
PRO_0000056579Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi210007.SMU.676.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93
Beta strandi12 – 143
Beta strandi17 – 237
Turni25 – 273
Beta strandi30 – 356
Helixi39 – 5820
Helixi61 – 7717
Helixi79 – 9012
Helixi94 – 11522
Beta strandi120 – 1245
Helixi125 – 1273
Helixi130 – 1323
Beta strandi135 – 1439
Beta strandi145 – 1506
Beta strandi153 – 1553
Helixi158 – 16912
Beta strandi173 – 1775
Turni180 – 1823
Helixi183 – 19614
Beta strandi202 – 2054
Helixi210 – 21910
Beta strandi225 – 2306
Helixi232 – 24110
Turni242 – 2443
Beta strandi247 – 2504
Beta strandi255 – 2595
Helixi265 – 27713
Helixi278 – 2814
Beta strandi284 – 29310
Helixi294 – 30916
Helixi316 – 3183
Helixi328 – 34316
Beta strandi347 – 3504
Beta strandi362 – 3665
Helixi372 – 3743
Beta strandi380 – 38910
Helixi391 – 40010
Beta strandi401 – 41010
Helixi414 – 42310
Beta strandi426 – 4338
Beta strandi451 – 4533
Helixi457 – 4637
Beta strandi465 – 4739

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUHX-ray1.82A/B/C/D1-475[»]
1QI1X-ray3.00A/B/C/D1-475[»]
1QI6X-ray2.50A/B/C/D1-475[»]
2ESDX-ray2.55A/B/C/D1-475[»]
2EUHX-ray2.60A/B/C/D1-475[»]
2ID2X-ray2.50A/B/C/D1-475[»]
2QE0X-ray2.19A/B/C/D1-475[»]
ProteinModelPortaliQ59931.
SMRiQ59931. Positions 2-475.

Miscellaneous databases

EvolutionaryTraceiQ59931.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 1552Substrate binding
Regioni283 – 2853Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1012.
KOiK00131.
OMAiLIAGEWC.
OrthoDBiEOG6BS8QW.
PhylomeDBiQ59931.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59931-1 [UniParc]FASTAAdd to Basket

« Hide

MTKQYKNYVN GEWKLSENEI KIYEPASGAE LGSVPAMSTE EVDYVYASAK    50
KAQPAWRSLS YIERAAYLHK VADILMRDKE KIGAVLSKEV AKGYKSAVSE 100
VVRTAEIINY AAEEGLRMEG EVLEGGSFEA ASKKKIAVVR REPVGLVLAI 150
SPFNYPVNLA GSKIAPALIA GNVIAFKPPT QGSISGLLLA EAFAEAGLPA 200
GVFNTITGRG SEIGDYIVEH QAVNFINFTG STGIGERIGK MAGMRPIMLE 250
LGGKDSAIVL EDADLELTAK NIIAGAFGYS GQRCTAVKRV LVMESVADEL 300
VEKIREKVLA LTIGNPEDDA DITPLIDTKS ADYVEGLIND ANDKGAAALT 350
EIKREGNLIC PILFDKVTTD MRLAWEEPFG PVLPIIRVTS VEEAIEISNK 400
SEYGLQASIF TNDFPRAFGI AEQLEVGTVH INNKTQRGTD NFPFLGAKKS 450
GAGIQGVKYS IEAMTTVKSV VFDIK 475
Length:475
Mass (Da):51,194
Last modified:November 28, 2002 - v2
Checksum:iF0A2770AB89552DC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581S → A in AAA91091. 1 Publication
Sequence conflicti85 – 851V → I in AAA91091. 1 Publication
Sequence conflicti347 – 3471A → T in AAA91091. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38521 Genomic DNA. Translation: AAA91091.1.
AE014133 Genomic DNA. Translation: AAN58410.1.
PIRiA57151.
RefSeqiNP_721104.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58410; AAN58410; SMU_676.
GeneIDi1028095.
KEGGismu:SMU_676.
PATRICi19663537. VBIStrMut61772_0601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38521 Genomic DNA. Translation: AAA91091.1 .
AE014133 Genomic DNA. Translation: AAN58410.1 .
PIRi A57151.
RefSeqi NP_721104.1. NC_004350.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EUH X-ray 1.82 A/B/C/D 1-475 [» ]
1QI1 X-ray 3.00 A/B/C/D 1-475 [» ]
1QI6 X-ray 2.50 A/B/C/D 1-475 [» ]
2ESD X-ray 2.55 A/B/C/D 1-475 [» ]
2EUH X-ray 2.60 A/B/C/D 1-475 [» ]
2ID2 X-ray 2.50 A/B/C/D 1-475 [» ]
2QE0 X-ray 2.19 A/B/C/D 1-475 [» ]
ProteinModelPortali Q59931.
SMRi Q59931. Positions 2-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.676.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58410 ; AAN58410 ; SMU_676 .
GeneIDi 1028095.
KEGGi smu:SMU_676.
PATRICi 19663537. VBIStrMut61772_0601.

Phylogenomic databases

eggNOGi COG1012.
KOi K00131.
OMAi LIAGEWC.
OrthoDBi EOG6BS8QW.
PhylomeDBi Q59931.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13095.
SMUT210007:GC7Z-660-MONOMER.
SABIO-RK Q59931.

Miscellaneous databases

EvolutionaryTracei Q59931.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, expression, and function of the gene for the nonphosphorylating, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Streptococcus mutans."
    Boyd D.A., Cvitkovitch D.G., Hamilton I.R.
    J. Bacteriol. 177:2622-2627(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NG5 / Serotype c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.
  3. "Apo and holo crystal structures of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans."
    Cobessi D., Tete-Favier F., Marchal S., Azza S., Branlant G., Aubry A.
    J. Mol. Biol. 290:161-173(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS) IN COMPLEX WITH NADP, HOMOTETRAMERIZATION.
  4. "Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans."
    Cobessi D., Tete-Favier F., Marchal S., Branlant G., Aubry A.
    J. Mol. Biol. 300:141-152(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH NADP AND SUBSTRATE.
  5. "Invariant Thr244 is essential for the efficient acylation step of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans."
    Pailot A., D'Ambrosio K., Corbier C., Talfournier F., Branlant G.
    Biochem. J. 400:521-530(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NADP, CHARACTERIZATION.

Entry informationi

Entry nameiGAPN_STRMU
AccessioniPrimary (citable) accession number: Q59931
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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