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Q59925 (FTHS_STRMU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Synonyms:fthS
Ordered Locus Names:SMU_1073
OrganismStreptococcus mutans serotype c (strain ATCC 700610 / UA159) [Complete proteome] [HAMAP]
Taxonomic identifier210007 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199386

Regions

Nucleotide binding65 – 728ATP By similarity

Experimental info

Sequence conflict251F → I in AAB49329. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q59925 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: E86BE44B307C099D

FASTA55659,734
        10         20         30         40         50         60 
MKTDIEIAQS VDLRPITNVV KKLGFDFDDL ELYGKYKAKL TFDKIKAVEE NAPGKLVLVT 

        70         80         90        100        110        120 
AINPTPAGEG KSTITIGLAD ALNKIGKKTM IAIREPSLGP VMGIKGGAAG GGYAQVLPME 

       130        140        150        160        170        180 
DINLHFTGDM HAITTANNAL SALIDNHLHQ GNELGIDQRR IIWKRVVDLN DRALRHVTVG 

       190        200        210        220        230        240 
LGSPINGIPR EDGFDITVAS EIMAILCLAT NVEDLKERLA NIVIGYRFDR SPVYVRDLEV 

       250        260        270        280        290        300 
QGALALILKE AIKPNLVQTI YGTPAFVHGG PFANIAHGCN SVLATSTALR LADYTITEAG 

       310        320        330        340        350        360 
FGADLGAEKF LDIKAPNLPT SPDAVVIVAT IRALKMNGGV AKDALNQENV EAVKAGFANL 

       370        380        390        400        410        420 
ARHVENMRKY GVPVVVAINE FITDTNDEIA VLRNLCAAID VPVELASVWA NGADGGVDLA 

       430        440        450        460        470        480 
NTLINTIENN PSHYKRLYDN NLSVEEKVTE IAKEIYRADK VIFEKKAKTQ IAQIVKNGWD 

       490        500        510        520        530        540 
NLPICMAKTQ YSFSDDPKLL GAPTGFDITI RELVPKLGAG FIVALTGDVM TMPGLPKKPA 

       550 
ALNMDVAADG TALGLF 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans."
Crowley P.J., Gutierrez J.A., Hillman J.D., Bleiweis A.S.
J. Bacteriol. 179:1563-1572(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JH1005.
[2]"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen."
Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.
Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700610 / UA159.
[3]"Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements."
Gutierrez J.A., Crowley P.J., Brown D.P., Hillman J.D., Youngman P., Bleiweis A.S.
J. Bacteriol. 178:4166-4175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 139-296.
Strain: NG8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39612 Genomic DNA. Translation: AAB49329.1.
AE014133 Genomic DNA. Translation: AAN58771.1.
U48882 Genomic DNA. Translation: AAC44499.1.
RefSeqNP_721465.1. NC_004350.2.

3D structure databases

ProteinModelPortalQ59925.
SMRQ59925. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING210007.SMU.1073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN58771; AAN58771; SMU_1073.
GeneID1028388.
KEGGsmu:SMU_1073.
PATRIC19664255. VBIStrMut61772_0960.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycSMUT210007:GC7Z-1021-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_STRMU
AccessionPrimary (citable) accession number: Q59925
Secondary accession number(s): Q59926
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways