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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-1021-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Synonyms:fthS
Ordered Locus Names:SMU_1073
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000199386Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_1073.

Structurei

3D structure databases

ProteinModelPortaliQ59925.
SMRiQ59925. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.
PhylomeDBiQ59925.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDIEIAQS VDLRPITNVV KKLGFDFDDL ELYGKYKAKL TFDKIKAVEE
60 70 80 90 100
NAPGKLVLVT AINPTPAGEG KSTITIGLAD ALNKIGKKTM IAIREPSLGP
110 120 130 140 150
VMGIKGGAAG GGYAQVLPME DINLHFTGDM HAITTANNAL SALIDNHLHQ
160 170 180 190 200
GNELGIDQRR IIWKRVVDLN DRALRHVTVG LGSPINGIPR EDGFDITVAS
210 220 230 240 250
EIMAILCLAT NVEDLKERLA NIVIGYRFDR SPVYVRDLEV QGALALILKE
260 270 280 290 300
AIKPNLVQTI YGTPAFVHGG PFANIAHGCN SVLATSTALR LADYTITEAG
310 320 330 340 350
FGADLGAEKF LDIKAPNLPT SPDAVVIVAT IRALKMNGGV AKDALNQENV
360 370 380 390 400
EAVKAGFANL ARHVENMRKY GVPVVVAINE FITDTNDEIA VLRNLCAAID
410 420 430 440 450
VPVELASVWA NGADGGVDLA NTLINTIENN PSHYKRLYDN NLSVEEKVTE
460 470 480 490 500
IAKEIYRADK VIFEKKAKTQ IAQIVKNGWD NLPICMAKTQ YSFSDDPKLL
510 520 530 540 550
GAPTGFDITI RELVPKLGAG FIVALTGDVM TMPGLPKKPA ALNMDVAADG

TALGLF
Length:556
Mass (Da):59,734
Last modified:November 28, 2002 - v2
Checksum:iE86BE44B307C099D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251F → I in AAB49329 (PubMed:9045814).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39612 Genomic DNA. Translation: AAB49329.1.
AE014133 Genomic DNA. Translation: AAN58771.1.
U48882 Genomic DNA. Translation: AAC44499.1.
RefSeqiNP_721465.1. NC_004350.2.
WP_002262267.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58771; AAN58771; SMU_1073.
GeneIDi1028388.
KEGGismu:SMU_1073.
PATRICi19664255. VBIStrMut61772_0960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39612 Genomic DNA. Translation: AAB49329.1.
AE014133 Genomic DNA. Translation: AAN58771.1.
U48882 Genomic DNA. Translation: AAC44499.1.
RefSeqiNP_721465.1. NC_004350.2.
WP_002262267.1. NC_004350.2.

3D structure databases

ProteinModelPortaliQ59925.
SMRiQ59925. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi210007.SMU_1073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN58771; AAN58771; SMU_1073.
GeneIDi1028388.
KEGGismu:SMU_1073.
PATRICi19664255. VBIStrMut61772_0960.

Phylogenomic databases

eggNOGiCOG2759.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.
PhylomeDBiQ59925.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSMUT210007:GC7Z-1021-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans."
    Crowley P.J., Gutierrez J.A., Hillman J.D., Bleiweis A.S.
    J. Bacteriol. 179:1563-1572(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JH1005.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.
  3. "Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements."
    Gutierrez J.A., Crowley P.J., Brown D.P., Hillman J.D., Youngman P., Bleiweis A.S.
    J. Bacteriol. 178:4166-4175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 139-296.
    Strain: NG8.

Entry informationi

Entry nameiFTHS_STRMU
AccessioniPrimary (citable) accession number: Q59925
Secondary accession number(s): Q59926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: July 22, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.