Reviewed, UniProtKB/Swiss-Prot Q59833 (GLGB1_STRCO)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 1
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 1
      Short name=BE 1
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1

Gene names

Name:	glgB1
Synonyms:	glgBI
Ordered Locus Names:	SCO5440
ORF Names:	SC6A11.16c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. This isozyme is involved in phase I (vegetative/aerial mycelium boundary) of glycogen synthesis. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	1,4-alpha-glucan-branching enzyme 1 HAMAP MF_00685		PRO_0000188749
Sites
	Active site	352	1	By similarity
	Active site	387	1	By similarity
	Active site	392	1	By similarity
	Active site	455	1	By similarity
	Active site	457	1	By similarity
	Active site	510	1	By similarity
	Active site	583	1	By similarity
	Active site	584	1	By similarity
Experimental info
	Sequence conflict	146	1	T → R in CAA58314. Ref.1
	Sequence conflict	146	1	T → R in CAA04603. Ref.1
	Sequence conflict	766 – 774	9	GTAARRASG → WHGRPASIRLTLPPLATVWL RPA in CAA58314. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59833-1 [UniParc]. Last modified August 15, 2003. Version 2. Checksum: 1D55555330F249BF	FASTA	774	85,451
	10 20 30 40 50 60 MTPRPSSSGP DPRKTTGKKP AGKTPTGKKP AKAAKKKAPR RTTASANASA TTSVSGAEVA 70 80 90 100 110 120 VSPAPDAADR ERLLAGTHHD PHAVLGAHRV PGGVAFRVFR PYALAVTVLS GELRVGLHDD 130 140 150 160 170 180 GDGFFSGLVP LKDVPAHRLL VAYEGTEQEV EDPYRFLPTL GELDLHLLGE GRHEQLWRAL 190 200 210 220 230 240 GAHPTTHEGV AGTRFAVWAP NARGVRVAGG FNFWDGTGHP MRSLGSTGVW ELFLPGVGAG 250 260 270 280 290 300 ELYKFEITRP DGSRTFRADP LARRTEVPPA TSSVVHASDY TWGDEEWLAH RADAPAHEAP 310 320 330 340 350 360 MSVYEVHLPS WRPGLTYRQL AEQLPAYVAD LGFTHVELMP VAEHPFGGSW GYQVTGFYAP 370 380 390 400 410 420 TARLGDPDDF KYLVDRLHRA GIGVLMDWVP AHFPRDDWAL AEFDGRPLYE HSDPLRAAHP 430 440 450 460 470 480 DWGTLEFDFG RREVRNFLVA NAVYWCEEFH IDGLRVDAVA SMLYLDYSRE PGEWEPNEHG 490 500 510 520 530 540 GRENLDAVAF LQEMNATLYR RVPGVVTVAE ESTAWDGVTR ATHHEGPSGF GGLGFGLKWN 550 560 570 580 590 600 MGWMHDSLDY MSHEPVHRKH HHGEMTFSMV YAYSENYVLP ISHDEVVHGK RSLVSKMPGD 610 620 630 640 650 660 WWQQRANERA YLGFMWAHPG KQLLFMGQEF AQGAEWSEAH GPDWWLLDPE YGASADHRGV 670 680 690 700 710 720 RDLVRDLNTV YRATPALWRR DTHPSGFSWV VGDAAEDNVL AFLRLDADGT PLLAVSNFAP 730 740 750 760 770 VVRSGYRLGV PDEVPAWHEV LNTDAARYGG GDVVNPDPVK PEPQGGTAAR RASG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific glycogen branching isoenzymes in a multicellular prokaryote, Streptomyces coelicolor A3(2)." Bruton C.J., Plaskitt K.A., Chater K.F. Mol. Microbiol. 18:89-99(1995) [PubMed: 8596463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2).
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"Duplicated gene clusters reveal a possible 'carbon relay' during aerial mycelium development in Streptomyces coelicolor A3(2)." Schneider D., Bruton C.J., Chater K.F. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-217. Strain: A3(2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83397 Genomic DNA. Translation: CAA58314.1. AL939123 Genomic DNA. Translation: CAB72416.1. AJ001205 Genomic DNA. Translation: CAA04603.1.
PIR	S70079.
RefSeq	NP_629578.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1100880.
GenomeReviews	Gene locus SCO5440 in contig AL645882_GR.
KEGG	sco:SCO5440.
NMPDR	fig|100226.1.peg.5392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59833.
OMA	GFDWSVA.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO5440-MON.
BRENDA	2.4.1.18. 1084.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_STRCO

Accession

Primary (citable) accession number: Q59833
Secondary accession number(s): Q9L1K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	August 15, 2003
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)