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Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei421 – 4211NucleophileBy similarity
Active sitei474 – 4741Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name:
BE 2
Gene namesi
Name:glgB2
Synonyms:glgBII
Ordered Locus Names:SCO7332
ORF Names:SC4G10.11c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7417411,4-alpha-glucan branching enzyme GlgB 2PRO_0000188750Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO7332.

Structurei

3D structure databases

ProteinModelPortaliQ59832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ59832.
KOiK00700.
OMAiYSRDSGQ.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ59832.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59832-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRDTSIPE PSGPVPPAPG ACATAPPLDP TDRGRLLAGA HHDPHSLLGA
60 70 80 90 100
HPVPGGIAFR VLRPFAREVG VVVDGERHTL ASEEDGLFSG VLPLAGIPSY
110 120 130 140 150
TLVVAYEQGE TQETHDPYRF LPALGELDLH LIGEGRHEQL WQALGAEPMT
160 170 180 190 200
HEGVTGTRFT VWAPNAQGVR VATDFTHWDG TAFPMRSLGS SGVWELFLPG
210 220 230 240 250
VGEGTRYKFE IHSRYGHRFL KADPMARAAE EPPNTASVVT ASRYEWGDAQ
260 270 280 290 300
WMRTRADTPV HEAPFSVYEV HLPSWRPGLT YRELAEELPA YVKDLGFTHV
310 320 330 340 350
ELMPVAEHPY GPSWGYQVTG FYAPTARLGS PDDFRFLVDA LHRAGIGVIM
360 370 380 390 400
DWVPAHFPKD DWALGRFDGD PLYEPGDSRR AEHPDWGTYT FDFARTEVRN
410 420 430 440 450
FLVANAVYWC EEFHIDGLRV DAVASMLYLD YSRDSGQWEP NQYGGREDLA
460 470 480 490 500
AMAFLQEMNA TVYRRCPGVV TIAEESTAWG GVTRPTDTGG LGFGLKWNMG
510 520 530 540 550
WMHDSLEYVA HEPVHRRYHH HEMTFSMVYA YSENYVLPIS HDEVVHGKQA
560 570 580 590 600
LVSKMPGDWW QRRANVRAYL GFMWAHPGKQ LLFMGQEFAQ GAEWSEKQGP
610 620 630 640 650
EWWLLDEGYH SAGDHRGVQD LVRELNTRYT RTPALWQRDT DPAGFRWVSV
660 670 680 690 700
DAAEDNVFAF LRYGTDGTPL LAVSNFSPVV RHEYGLAVGD EAVAWQEVLN
710 720 730 740
TDAEEYGGGG VGNPDPVKPE DGSIRITLPP LATVWLMPYA L
Length:741
Mass (Da):82,776
Last modified:August 15, 2003 - v2
Checksum:i5FB7A44120677759
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti561 – 5611Q → H in CAA52109. (PubMed:8596463)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73903 Genomic DNA. Translation: CAA52109.1.
AL939131 Genomic DNA. Translation: CAB92878.1.
PIRiS70078. S34218.
RefSeqiNP_631386.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB92878; CAB92878; CAB92878.
GeneIDi1102770.
KEGGisco:SCO7332.
PATRICi23744587. VBIStrCoe124346_7436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73903 Genomic DNA. Translation: CAA52109.1.
AL939131 Genomic DNA. Translation: CAB92878.1.
PIRiS70078. S34218.
RefSeqiNP_631386.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ59832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO7332.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB92878; CAB92878; CAB92878.
GeneIDi1102770.
KEGGisco:SCO7332.
PATRICi23744587. VBIStrCoe124346_7436.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ59832.
KOiK00700.
OMAiYSRDSGQ.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ59832.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tissue-specific glycogen branching isoenzymes in a multicellular prokaryote, Streptomyces coelicolor A3(2)."
    Bruton C.J., Plaskitt K.A., Chater K.F.
    Mol. Microbiol. 18:89-99(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiGLGB2_STRCO
AccessioniPrimary (citable) accession number: Q59832
Secondary accession number(s): Q9KY06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: August 15, 2003
Last modified: January 7, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.