1,4-alpha-glucan branching enzyme GlgB 2

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Q59832 (GLGB2_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2

Gene names

Name:	glgB2
Synonyms:	glgBII
Ordered Locus Names:	SCO7332
ORF Names:	SC4G10.11c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 741

741

1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685

PRO_0000188750

Sites

Active site

421

Nucleophile By similarity

Active site

474

Proton donor By similarity

Experimental info

Sequence conflict

561

Q → H in CAA52109. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q59832 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 5FB7A44120677759
Show »

FASTA

741

82,776

        10         20         30         40         50         60 
MALRDTSIPE PSGPVPPAPG ACATAPPLDP TDRGRLLAGA HHDPHSLLGA HPVPGGIAFR 

        70         80         90        100        110        120 
VLRPFAREVG VVVDGERHTL ASEEDGLFSG VLPLAGIPSY TLVVAYEQGE TQETHDPYRF 

       130        140        150        160        170        180 
LPALGELDLH LIGEGRHEQL WQALGAEPMT HEGVTGTRFT VWAPNAQGVR VATDFTHWDG 

       190        200        210        220        230        240 
TAFPMRSLGS SGVWELFLPG VGEGTRYKFE IHSRYGHRFL KADPMARAAE EPPNTASVVT 

       250        260        270        280        290        300 
ASRYEWGDAQ WMRTRADTPV HEAPFSVYEV HLPSWRPGLT YRELAEELPA YVKDLGFTHV 

       310        320        330        340        350        360 
ELMPVAEHPY GPSWGYQVTG FYAPTARLGS PDDFRFLVDA LHRAGIGVIM DWVPAHFPKD 

       370        380        390        400        410        420 
DWALGRFDGD PLYEPGDSRR AEHPDWGTYT FDFARTEVRN FLVANAVYWC EEFHIDGLRV 

       430        440        450        460        470        480 
DAVASMLYLD YSRDSGQWEP NQYGGREDLA AMAFLQEMNA TVYRRCPGVV TIAEESTAWG 

       490        500        510        520        530        540 
GVTRPTDTGG LGFGLKWNMG WMHDSLEYVA HEPVHRRYHH HEMTFSMVYA YSENYVLPIS 

       550        560        570        580        590        600 
HDEVVHGKQA LVSKMPGDWW QRRANVRAYL GFMWAHPGKQ LLFMGQEFAQ GAEWSEKQGP 

       610        620        630        640        650        660 
EWWLLDEGYH SAGDHRGVQD LVRELNTRYT RTPALWQRDT DPAGFRWVSV DAAEDNVFAF 

       670        680        690        700        710        720 
LRYGTDGTPL LAVSNFSPVV RHEYGLAVGD EAVAWQEVLN TDAEEYGGGG VGNPDPVKPE 

       730        740 
DGSIRITLPP LATVWLMPYA L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific glycogen branching isoenzymes in a multicellular prokaryote, Streptomyces coelicolor A3(2)." Bruton C.J., Plaskitt K.A., Chater K.F. Mol. Microbiol. 18:89-99(1995) [PubMed: 8596463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73903 Genomic DNA. Translation: CAA52109.1. AL939131 Genomic DNA. Translation: CAB92878.1.
PIR	S34218. S70078.
RefSeq	NP_631386.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q59832.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1102770.
GenomeReviews	Gene locus SCO7332 in contig AL645882_GR.
KEGG	sco:SCO7332.
NMPDR	fig\|100226.1.peg.7266.
PATRIC	23744587. VBIStrCoe124346_7436.
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RTHEAGF.
PhylomeDB	Q59832.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_STRCO

Accession

Primary (citable) accession number: Q59832
Secondary accession number(s): Q9KY06

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	August 15, 2003
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents