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Q59832 (GLGB2_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 2

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2
Gene names
Name:glgB2
Synonyms:glgBII
Ordered Locus Names:SCO7332
ORF Names:SC4G10.11c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7417411,4-alpha-glucan branching enzyme GlgB 2 HAMAP-Rule MF_00685
PRO_0000188750

Sites

Active site4211Nucleophile By similarity
Active site4741Proton donor By similarity

Experimental info

Sequence conflict5611Q → H in CAA52109. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q59832 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 5FB7A44120677759

FASTA74182,776
        10         20         30         40         50         60 
MALRDTSIPE PSGPVPPAPG ACATAPPLDP TDRGRLLAGA HHDPHSLLGA HPVPGGIAFR 

        70         80         90        100        110        120 
VLRPFAREVG VVVDGERHTL ASEEDGLFSG VLPLAGIPSY TLVVAYEQGE TQETHDPYRF 

       130        140        150        160        170        180 
LPALGELDLH LIGEGRHEQL WQALGAEPMT HEGVTGTRFT VWAPNAQGVR VATDFTHWDG 

       190        200        210        220        230        240 
TAFPMRSLGS SGVWELFLPG VGEGTRYKFE IHSRYGHRFL KADPMARAAE EPPNTASVVT 

       250        260        270        280        290        300 
ASRYEWGDAQ WMRTRADTPV HEAPFSVYEV HLPSWRPGLT YRELAEELPA YVKDLGFTHV 

       310        320        330        340        350        360 
ELMPVAEHPY GPSWGYQVTG FYAPTARLGS PDDFRFLVDA LHRAGIGVIM DWVPAHFPKD 

       370        380        390        400        410        420 
DWALGRFDGD PLYEPGDSRR AEHPDWGTYT FDFARTEVRN FLVANAVYWC EEFHIDGLRV 

       430        440        450        460        470        480 
DAVASMLYLD YSRDSGQWEP NQYGGREDLA AMAFLQEMNA TVYRRCPGVV TIAEESTAWG 

       490        500        510        520        530        540 
GVTRPTDTGG LGFGLKWNMG WMHDSLEYVA HEPVHRRYHH HEMTFSMVYA YSENYVLPIS 

       550        560        570        580        590        600 
HDEVVHGKQA LVSKMPGDWW QRRANVRAYL GFMWAHPGKQ LLFMGQEFAQ GAEWSEKQGP 

       610        620        630        640        650        660 
EWWLLDEGYH SAGDHRGVQD LVRELNTRYT RTPALWQRDT DPAGFRWVSV DAAEDNVFAF 

       670        680        690        700        710        720 
LRYGTDGTPL LAVSNFSPVV RHEYGLAVGD EAVAWQEVLN TDAEEYGGGG VGNPDPVKPE 

       730        740 
DGSIRITLPP LATVWLMPYA L 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73903 Genomic DNA. Translation: CAA52109.1.
AL939131 Genomic DNA. Translation: CAB92878.1.
PIRS34218. S70078.
RefSeqNP_631386.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ59832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO7332.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB92878; CAB92878; CAB92878.
GeneID1102770.
KEGGsco:SCO7332.
PATRIC23744587. VBIStrCoe124346_7436.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHENIENP.
OrthoDBEOG6JX7GT.
PhylomeDBQ59832.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_STRCO
AccessionPrimary (citable) accession number: Q59832
Secondary accession number(s): Q9KY06
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: August 15, 2003
Last modified: June 11, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries