Reviewed, UniProtKB/Swiss-Prot Q59801 (HYSA_STAA8)

Names and origin

Protein names

Recommended name:
    Hyaluronate lyase
    EC=4.2.2.1
Alternative name(s):
    Hyaluronidase
      Short name=HYase

Gene names

Name:	hysA
Ordered Locus Names:	SAOUHSC_02463

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 8 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hyaluronate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Potential
	Chain	41 – 807	767	Hyaluronate lyase		PRO_0000024931

Sequences

	Sequence		Length	Mass (Da)
	Q59801-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FFE88EDC07418EB4	FASTA	807	91,985
	10 20 30 40 50 60 MTYRIKKWQK LSTITLLMAG VITLNGGEFR SVDKHQIAVA DTNVQTPDYE KLRNTWLDVN 70 80 90 100 110 120 YGYDKYDENN PDMKKKFDAT EKEATNLLKE MKTESGRKYL WSGAETLETN SSHMTRTYRN 130 140 150 160 170 180 IEKIAEAMRN PKTTLNTDEN KKKVKDALEW LHKNAYGKEP DKKVKELSEN FTKTTGKNTN 190 200 210 220 230 240 LNWWDYEIGT PKSLTNTLIL LNDQFSNEEK KKFTAPIKTF APDSDKILSS VGKAELAKGG 250 260 270 280 290 300 NLVDISKVKL LECIIEEDKD MMKKSIDSFN KVFTYVQDSA TGKERNGFYK DGSYIDHQDV 310 320 330 340 350 360 PYTGAYGVVL LEGISQMMPM IKETPFNDKT QNDTTLKSWI DDGFMPLIYK GEMMDLSRGR 370 380 390 400 410 420 AISRENETSH SASATVMKSL LRLSDAMDDS TKAKYKKIVK SSVESDSSYK QNDYLNSYSD 430 440 450 460 470 480 IDKMKSLMTD NSISKNGLTQ QLKIYNDMDR VTYHNKDLDF AFGLSMTSKN VARYESINGE 490 500 510 520 530 540 NLKGWHTGAG MSYLYNSDVK HYHDNFWVTA DMKRLSGTTT LDNEILKDTD DKKSSKTFVG 550 560 570 580 590 600 GTKVDDQHAS IGMDFENQDK TLTAKKSYFI LNDKIVFLGT GIKSTDSSKN PVTTIENRKA 610 620 630 640 650 660 NGYTLYTDDK QTTNSDNQEN NSVFLESTDT KKNIGYHFLN KPKITVKKES HTGKWKEINK 670 680 690 700 710 720 SQKDTQKTDE YYEVTQKHSN SDNKYGYVLY PGLSKDVFKT KKDEVTVVKQ EDDFHVVKDN 730 740 750 760 770 780 ESVWAGVNYS NSTQTFDINN TKVEVKAKGM FILKKKDDNT YECSFYNPES TNSASDIESK 790 800 ISMTGYSITN KNTSTSNESG VHFELTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence determination and expression of the Staphylococcus aureus hyaluronate lyase gene." Farrell A.M., Taylor D., Holland K.T. FEMS Microbiol. Lett. 130:81-85(1995) [PubMed: 7557301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U21221 Genomic DNA. Translation: AAA82984.1. CP000253 Genomic DNA. Translation: ABD31483.1.
RefSeq	YP_500931.1.
3D structure databases
HSSP	HSSP built from PDB template 1LXM based on UniProtKB Q53591.
ModBase	Search...
Protein family/group databases
CAZy	PL8. Polysaccharide Lyase Family 8.
Genome annotation databases
GeneID	3919026.
GenomeReviews	Gene locus SAOUHSC_02463 in contig CP000253_GR.
KEGG	sao:SAOUHSC_02463.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59801.
OMA	Q59801. GELMDMS.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_02463-MON.
Family and domain databases
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR012970. Lyase_8_alpha_N. IPR004103. Lyase_8_C. IPR003159. Lyase_8_central. IPR012329. Lyase_8_N. [Graphical view]
Gene3D	G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:2.60.220.10. Lyase_8_C. 1 hit. G3DSA:1.50.10.100. Lyase_8_N. 1 hit.
Pfam	PF02278. Lyase_8. 1 hit. PF02884. Lyase_8_C. 1 hit. PF08124. Lyase_8_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYSA_STAA8

Accession

Primary (citable) accession number: Q59801
Secondary accession number(s): Q2FW54

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)