Reviewed, UniProtKB/Swiss-Prot Q59767 (NADB_RHORU)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:

nadB

Organism

Rhodospirillum rubrum

Taxonomic identifier

1085 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	277 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 277	›277	L-aspartate oxidase		PRO_0000184398
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q59767-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 253364CE414F5204	FASTA	277	28,540
	10 20 30 40 50 60 ILVDAQGRRF MTALDPAAEL APRDVVTRGV AAAIKAGKGA FLDARTALGA AFPQAFPTVY 70 80 90 100 110 120 AACRASGIDP VRQPIPIAPA AHYHMGGVLT DSFGRTSIDG LWAVGEVACT GAHGANRLAS 130 140 150 160 170 180 NSLLEAVVFA ARVAGDVGAA PLRRGWPPPA PPDLSPRPPP AEDAAAITRL RATMSARVGV 190 200 210 220 230 240 IRDGQGLESA LATIETIAAG ARSPRLRAMT TAARLITAAA LARRESRGAH FRADYPATDP 250 260 270 AQSHRATAPF GPLRTVPPHP AQSLGEGLAA PPMETRS

References

[1]

"Carbon monoxide-induced activation of gene expression in Rhodospirillum rubrum requires the product of cooA, a member of the cyclic AMP receptor protein family of transcriptional regulators." Shelver D., Kerby R.L., He Y., Roberts G.P. J. Bacteriol. 177:2157-2163(1995) [PubMed: 7721706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UR1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U65510 Genomic DNA. Translation: AAC45129.1.
PIR	T51327.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.3.16. 1671.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NADB_RHORU

Accession

Primary (citable) accession number: Q59767

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)