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Q59750

- BGAL_RHIML

UniProt

Q59750 - BGAL_RHIML

Protein

Beta-galactosidase

Gene

lacZ

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei382 – 3821Proton donorBy similarity
    Active sitei463 – 4631NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:lacZ
    OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri382 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 755755Beta-galactosidasePRO_0000057674Add
    BLAST

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ59750.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59750-1 [UniParc]FASTAAdd to Basket

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    MRSVTSFNDS WVFSEASTRD AERSGRVSRS ACRTNAVELP FNYFDERCYQ    50
    RAFTYQRVLA WRPDFSQGSR SSSTRQWPMR SCISTAKRSS RIRDGYTPFE 100
    ARLTDRLLEG DNLITVKIDG SENPEIPPFG AGIDYLTYAG IYRDVWLKVT 150
    DPVSIANIKI ETRDVLSDHK AVSLRCDLSN PQGLSFSGTI SALLKNAAGE 200
    VLAEVAGETT GQSLAFEMDG LRGLSLWDID DPVLYVIEVE LRTGQGFRLL 250
    RRAFRLPHGE FTTEGFRLNG RPLKIRGLNR HQSFPYVGLR MGRTAKGSAH 300
    ADIMNAHRLH CNLVRTSHYP QSKWFLDHCD RIGLLVFARN PRLAAYRWGG 350
    METGGNPERP PHRSSATGTT RLSYIWGVRI NESQDSHDFY AETNRLAREL 400
    DPTRQTGGVR YITDSEFLED VYTMNDFILG NEELPGANRP GTALRPQQEC 450
    TGLPRKVPYL ITEFGGHMYP TKIYDQEQRQ AEHVRRHLEV LNAAYARNPG 500
    ISGAIGWCMF DYNTTRISAP ATGSAITASW TCSASPKFAA YVYASQCDPS 550
    EEIVMKPVTF WARGDDDIGG VLPLIVLTNC DEIELKYGSL TKRVGPDREN 600
    FPHLPHPPVV IDHRHFTKDE LGVWGMKWES AEFTGFIAGK PVADLRMAAD 650
    PVPTTLQVEA DSKTLRREGR DTVRLILRAL DQAGNVLPFL NDAVDIEIHG 700
    PARLVGPARI VLQGGSGFLA GVHGRRRHAS SRSRRRGSAA AKLDLVALAD 750
    GAASA 755
    Length:755
    Mass (Da):84,149
    Last modified:November 1, 1996 - v1
    Checksum:i09E339A92CC0D366
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20757 Genomic DNA. Translation: AAA26296.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20757 Genomic DNA. Translation: AAA26296.1 .

    3D structure databases

    ProteinModelPortali Q59750.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q59750.
    ChEMBLi CHEMBL3484.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide and deduced amino acid sequences of Rhizobium meliloti 102F34 lacZ gene: comparison with prokaryotic beta-galactosidases and human beta-glucuronidase."
      Fanning S., Leahy M., Sheehan D.
      Gene 141:91-96(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: 102F34.

    Entry informationi

    Entry nameiBGAL_RHIML
    AccessioniPrimary (citable) accession number: Q59750
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3