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Protein

Beta-galactosidase

Gene

lacZ

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei382 – 3821Proton donorBy similarity
Active sitei463 – 4631NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri382 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 755755Beta-galactosidasePRO_0000057674Add
BLAST

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ59750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSVTSFNDS WVFSEASTRD AERSGRVSRS ACRTNAVELP FNYFDERCYQ
60 70 80 90 100
RAFTYQRVLA WRPDFSQGSR SSSTRQWPMR SCISTAKRSS RIRDGYTPFE
110 120 130 140 150
ARLTDRLLEG DNLITVKIDG SENPEIPPFG AGIDYLTYAG IYRDVWLKVT
160 170 180 190 200
DPVSIANIKI ETRDVLSDHK AVSLRCDLSN PQGLSFSGTI SALLKNAAGE
210 220 230 240 250
VLAEVAGETT GQSLAFEMDG LRGLSLWDID DPVLYVIEVE LRTGQGFRLL
260 270 280 290 300
RRAFRLPHGE FTTEGFRLNG RPLKIRGLNR HQSFPYVGLR MGRTAKGSAH
310 320 330 340 350
ADIMNAHRLH CNLVRTSHYP QSKWFLDHCD RIGLLVFARN PRLAAYRWGG
360 370 380 390 400
METGGNPERP PHRSSATGTT RLSYIWGVRI NESQDSHDFY AETNRLAREL
410 420 430 440 450
DPTRQTGGVR YITDSEFLED VYTMNDFILG NEELPGANRP GTALRPQQEC
460 470 480 490 500
TGLPRKVPYL ITEFGGHMYP TKIYDQEQRQ AEHVRRHLEV LNAAYARNPG
510 520 530 540 550
ISGAIGWCMF DYNTTRISAP ATGSAITASW TCSASPKFAA YVYASQCDPS
560 570 580 590 600
EEIVMKPVTF WARGDDDIGG VLPLIVLTNC DEIELKYGSL TKRVGPDREN
610 620 630 640 650
FPHLPHPPVV IDHRHFTKDE LGVWGMKWES AEFTGFIAGK PVADLRMAAD
660 670 680 690 700
PVPTTLQVEA DSKTLRREGR DTVRLILRAL DQAGNVLPFL NDAVDIEIHG
710 720 730 740 750
PARLVGPARI VLQGGSGFLA GVHGRRRHAS SRSRRRGSAA AKLDLVALAD

GAASA
Length:755
Mass (Da):84,149
Last modified:November 1, 1996 - v1
Checksum:i09E339A92CC0D366
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20757 Genomic DNA. Translation: AAA26296.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20757 Genomic DNA. Translation: AAA26296.1.

3D structure databases

ProteinModelPortaliQ59750.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ59750.
ChEMBLiCHEMBL3484.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide and deduced amino acid sequences of Rhizobium meliloti 102F34 lacZ gene: comparison with prokaryotic beta-galactosidases and human beta-glucuronidase."
    Fanning S., Leahy M., Sheehan D.
    Gene 141:91-96(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: 102F34.

Entry informationi

Entry nameiBGAL_RHIML
AccessioniPrimary (citable) accession number: Q59750
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.