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Q59750 (BGAL_RHIML) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifier382 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 755755Beta-galactosidase
PRO_0000057674

Sites

Active site3821Proton donor By similarity
Active site4631Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59750 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 09E339A92CC0D366

FASTA75584,149
        10         20         30         40         50         60 
MRSVTSFNDS WVFSEASTRD AERSGRVSRS ACRTNAVELP FNYFDERCYQ RAFTYQRVLA 

        70         80         90        100        110        120 
WRPDFSQGSR SSSTRQWPMR SCISTAKRSS RIRDGYTPFE ARLTDRLLEG DNLITVKIDG 

       130        140        150        160        170        180 
SENPEIPPFG AGIDYLTYAG IYRDVWLKVT DPVSIANIKI ETRDVLSDHK AVSLRCDLSN 

       190        200        210        220        230        240 
PQGLSFSGTI SALLKNAAGE VLAEVAGETT GQSLAFEMDG LRGLSLWDID DPVLYVIEVE 

       250        260        270        280        290        300 
LRTGQGFRLL RRAFRLPHGE FTTEGFRLNG RPLKIRGLNR HQSFPYVGLR MGRTAKGSAH 

       310        320        330        340        350        360 
ADIMNAHRLH CNLVRTSHYP QSKWFLDHCD RIGLLVFARN PRLAAYRWGG METGGNPERP 

       370        380        390        400        410        420 
PHRSSATGTT RLSYIWGVRI NESQDSHDFY AETNRLAREL DPTRQTGGVR YITDSEFLED 

       430        440        450        460        470        480 
VYTMNDFILG NEELPGANRP GTALRPQQEC TGLPRKVPYL ITEFGGHMYP TKIYDQEQRQ 

       490        500        510        520        530        540 
AEHVRRHLEV LNAAYARNPG ISGAIGWCMF DYNTTRISAP ATGSAITASW TCSASPKFAA 

       550        560        570        580        590        600 
YVYASQCDPS EEIVMKPVTF WARGDDDIGG VLPLIVLTNC DEIELKYGSL TKRVGPDREN 

       610        620        630        640        650        660 
FPHLPHPPVV IDHRHFTKDE LGVWGMKWES AEFTGFIAGK PVADLRMAAD PVPTTLQVEA 

       670        680        690        700        710        720 
DSKTLRREGR DTVRLILRAL DQAGNVLPFL NDAVDIEIHG PARLVGPARI VLQGGSGFLA 

       730        740        750 
GVHGRRRHAS SRSRRRGSAA AKLDLVALAD GAASA 

« Hide

References

[1]"Nucleotide and deduced amino acid sequences of Rhizobium meliloti 102F34 lacZ gene: comparison with prokaryotic beta-galactosidases and human beta-glucuronidase."
Fanning S., Leahy M., Sheehan D.
Gene 141:91-96(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: 102F34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20757 Genomic DNA. Translation: AAA26296.1.

3D structure databases

ProteinModelPortalQ59750.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ59750.
ChEMBLCHEMBL3484.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_RHIML
AccessionPrimary (citable) accession number: Q59750
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries