Q59750 (BGAL_RHIML) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:

lacZ

Organism

Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Beta-galactosidase		PRO_0000057674
Sites
	Active site	382	1	Proton donor By similarity
	Active site	463	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59750 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 09E339A92CC0D366	FASTA	755	84,149
	10 20 30 40 50 60 MRSVTSFNDS WVFSEASTRD AERSGRVSRS ACRTNAVELP FNYFDERCYQ RAFTYQRVLA 70 80 90 100 110 120 WRPDFSQGSR SSSTRQWPMR SCISTAKRSS RIRDGYTPFE ARLTDRLLEG DNLITVKIDG 130 140 150 160 170 180 SENPEIPPFG AGIDYLTYAG IYRDVWLKVT DPVSIANIKI ETRDVLSDHK AVSLRCDLSN 190 200 210 220 230 240 PQGLSFSGTI SALLKNAAGE VLAEVAGETT GQSLAFEMDG LRGLSLWDID DPVLYVIEVE 250 260 270 280 290 300 LRTGQGFRLL RRAFRLPHGE FTTEGFRLNG RPLKIRGLNR HQSFPYVGLR MGRTAKGSAH 310 320 330 340 350 360 ADIMNAHRLH CNLVRTSHYP QSKWFLDHCD RIGLLVFARN PRLAAYRWGG METGGNPERP 370 380 390 400 410 420 PHRSSATGTT RLSYIWGVRI NESQDSHDFY AETNRLAREL DPTRQTGGVR YITDSEFLED 430 440 450 460 470 480 VYTMNDFILG NEELPGANRP GTALRPQQEC TGLPRKVPYL ITEFGGHMYP TKIYDQEQRQ 490 500 510 520 530 540 AEHVRRHLEV LNAAYARNPG ISGAIGWCMF DYNTTRISAP ATGSAITASW TCSASPKFAA 550 560 570 580 590 600 YVYASQCDPS EEIVMKPVTF WARGDDDIGG VLPLIVLTNC DEIELKYGSL TKRVGPDREN 610 620 630 640 650 660 FPHLPHPPVV IDHRHFTKDE LGVWGMKWES AEFTGFIAGK PVADLRMAAD PVPTTLQVEA 670 680 690 700 710 720 DSKTLRREGR DTVRLILRAL DQAGNVLPFL NDAVDIEIHG PARLVGPARI VLQGGSGFLA 730 740 750 GVHGRRRHAS SRSRRRGSAA AKLDLVALAD GAASA

References

[1]

"Nucleotide and deduced amino acid sequences of Rhizobium meliloti 102F34 lacZ gene: comparison with prokaryotic beta-galactosidases and human beta-glucuronidase." Fanning S., Leahy M., Sheehan D. Gene 141:91-96(1994) [PubMed: 8163182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: 102F34.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20757 Genomic DNA. Translation: AAA26296.1.
3D structure databases
ProteinModelPortal	Q59750.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_RHIML

Accession

Primary (citable) accession number: Q59750

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program