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Q59747

- GLNA1_RHIME

UniProt

Q59747 - GLNA1_RHIME

Protein

Glutamine synthetase 1

Gene

glnA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-1681-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 1 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase I
    Glutamine synthetase I
    Short name:
    GSI
    Gene namesi
    Name:glnA
    Ordered Locus Names:R01640
    ORF Names:SMc00948
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamine synthetase 1PRO_0000153225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei398 – 3981O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

    Protein-protein interaction databases

    STRINGi266834.SMc00948.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59747.
    SMRiQ59747. Positions 3-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005157.
    KOiK01915.
    OMAiDMLLMPI.
    OrthoDBiEOG6B360N.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59747-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTANEVLKQ IKENDVKFVD LRFTDPKGKL QHVTMDVVCV DEDMFADGVM    50
    FDGSSIGGWK AINESDMVLM PDPETAHMDP FFAQSTMVIF CDILDPVSGE 100
    AYNRDPRGTA KKAEAYLKAS GIGDTVFVGP EAEFFVFDDV KYKADPYNTG 150
    FKLDSSELPS NDDTDYETGN LGHRPRVKGG YFPVPPVDSS QDMRSEMLTV 200
    LSEMGVTVEK HHHEVAAAQH ELGVKFDALV RNADKMQIYK YVVHQVANAY 250
    GKTATFMPKP IFGDNGSGMH VHLSIWKDGK PTFAGDEYAG LSESCLYFIG 300
    GIIKHAKALN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPFGT 350
    NPKAKRVEVR FPDPTANPYL AFAAMLMAGL DGIKNKLHPG KAMDKDLYDL 400
    PPKELKKIPT VCGSLREALE SLDKDRKFLT AGGVFDDDQI DSFIELKMQE 450
    VMRFEMTPHP VEFDMYYSV 469
    Length:469
    Mass (Da):52,122
    Last modified:May 30, 2000 - v2
    Checksum:iFD1516B434FDF7A3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti354 – 3563AKR → PNG in AAC44624. (PubMed:8931324)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U50385 Genomic DNA. Translation: AAC44624.1.
    AL591688 Genomic DNA. Translation: CAC46219.1.
    AF169573 Genomic DNA. Translation: AAF18968.1.
    RefSeqiNP_385746.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC46219; CAC46219; SMc00948.
    GeneIDi1233299.
    KEGGisme:SMc00948.
    PATRICi23632655. VBISinMel96828_3073.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U50385 Genomic DNA. Translation: AAC44624.1 .
    AL591688 Genomic DNA. Translation: CAC46219.1 .
    AF169573 Genomic DNA. Translation: AAF18968.1 .
    RefSeqi NP_385746.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali Q59747.
    SMRi Q59747. Positions 3-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc00948.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC46219 ; CAC46219 ; SMc00948 .
    GeneIDi 1233299.
    KEGGi sme:SMc00948.
    PATRICi 23632655. VBISinMel96828_3073.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005157.
    KOi K01915.
    OMAi DMLLMPI.
    OrthoDBi EOG6B360N.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-1681-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Symbiotic nitrogen fixation does not require adenylylation of glutamine synthetase I in Rhizobium meliloti."
      Arcondeguy T., Huez I., Fourment J., Kahn D.
      FEMS Microbiol. Lett. 145:33-40(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RCR2011 / SU47.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    4. "The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications."
      Turner S.L., Young J.P.W.
      Mol. Biol. Evol. 17:309-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-362.
      Strain: ATCC 9930 / USDA 1002 / DSM 30135 / JCM 20682 / LMG 6133 / NBRC 14782 / NRRL L-45.

    Entry informationi

    Entry nameiGLNA1_RHIME
    AccessioniPrimary (citable) accession number: Q59747
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3