Reviewed, UniProtKB/Swiss-Prot Q59746 (NOSZ_RHIME)

Names and origin

Protein names

Recommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase

Gene names

Name:	nosZ
Ordered Locus Names:	RA0643
ORF Names:	SMa1182

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.
Catalytic activity	N(2) + H(2)O + acceptor = N(2)O + reduced acceptor.
Cofactor	Binds 2 calcium ions per subunit By similarity. Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.
Subunit structure	Homodimer By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the nosZ family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: HAMAP copper ion binding Inferred from electronic annotation. Source: HAMAP cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro nitrous-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 46	46	Tat-type signal Potential
	Chain	47 – 639	593	Nitrous-oxide reductase		PRO_0000019833
Regions
	Region	541 – 639	99	COX2-like
Sites
	Metal binding	136	1	Copper Z2 By similarity
	Metal binding	137	1	Copper Z3 By similarity
	Metal binding	185	1	Copper Z2 By similarity
	Metal binding	262	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	265	1	Calcium 2 By similarity
	Metal binding	273	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	279	1	Calcium 2 By similarity
	Metal binding	324	1	Calcium 2 By similarity
	Metal binding	326	1	Copper Z1 By similarity
	Metal binding	381	1	Copper Z1 By similarity
	Metal binding	432	1	Copper Z3 By similarity
	Metal binding	453	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	468	1	Calcium 1 By similarity
	Metal binding	493	1	Copper Z4 By similarity
	Metal binding	582	1	Copper A1 By similarity
	Metal binding	617	1	Copper A1 By similarity
	Metal binding	617	1	Copper A2 By similarity
	Metal binding	619	1	Copper A2; via carbonyl oxygen By similarity
	Metal binding	621	1	Copper A1 By similarity
	Metal binding	621	1	Copper A2 By similarity
	Metal binding	625	1	Copper A2 By similarity
	Metal binding	628	1	Copper A1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59746-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EBCF5EDFAFF70B96	FASTA	639	70,760
	10 20 30 40 50 60 MSNEETKMRL NRRQMLGTTA FMAAAGAVGA GGALTLSGGT ATPARAQETS GSSYEVKPGE 70 80 90 100 110 120 LDEYYVFFSS GQSGEIRIVG APSMREMMRI PVFNRCSATG WGQTNESRKV MTEGLLPETV 130 140 150 160 170 180 EFLKDQGGLY LNGDLHHPHP SFTDGTYDGR YLYANDKSNS RVCRIRLDVM KCDKIIQLPN 190 200 210 220 230 240 QHTVHGLRVQ KYPKTGYVFC NGEDAVPVPN DGKTMGDKNS YQAIFTAVDG ETMEVAWQVM 250 260 270 280 290 300 VDGNLDNVDA DYQGKYCFAT CYNSEEGFTL ADMMASEQDW VVIFNLKRIE EAVAKGDYKE 310 320 330 340 350 360 IGGVPVLDGR KGSPYTRYVP VPNSPHGINT APDGIHVVAN GKLSPTVTVF DVRKFDDLFD 370 380 390 400 410 420 DKIQARDTVV AEPELGLGPL HTAYDGKGNA YTTLFIDSQV CKWNIEDAKR AYAGEKVDPI 430 440 450 460 470 480 RHKLDVHYQP GHNHTSMGQT KEADGKWLIS LNKFSKDRYL NVGPLKPEND QLIDISGDEM 490 500 510 520 530 540 VLVHDNPTFA EPHDATIVHA SKINPVHVWN RDDPFFADAV AQAKADNIDL MVDSEVIRDG 550 560 570 580 590 600 NKVRVYMTSA APAFGLDDFT VKQGDEVTVY VTNIDEVEDL THGFCIVNYG INMEVAPQAT 610 620 630 ASVTFKASRP GVYWYYCTWF CHAMHMEMKG RMLVEAQGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and analysis of the dissimilatory nitrous oxide reduction genes, nosRZDFY, of Rhizobium meliloti." Holloway P., McCormick W., Watson R.J., Chan Y.K. J. Bacteriol. 178:1505-1514(1996) [PubMed: 8626275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ1c10.
[2]	"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47133 Genomic DNA. Translation: AAC44023.1. AE006469 Genomic DNA. Translation: AAK65301.1.
PIR	C95342.
RefSeq	NP_435889.1.
3D structure databases
HSSP	HSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBase	Search...
Genome annotation databases
GeneID	1235679.
GenomeReviews	Gene locus RA0643 in contig AE006469_GR.
KEGG	sme:SMa1182.
NMPDR	fig|266834.1.peg.643.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59746.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1182-MON.
Family and domain databases
HAMAP	MF_00716. [Tree]
InterPro	IPR001505. Copper_CuA. IPR002429. COX2_C. IPR008972. Cupredoxin. IPR015943. WD40/YVTN_repeat-like. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00116. COX2. 1 hit. [Graphical view]
PROSITE	PS00078. COX2. False negative. PS50857. COX2_CUA. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOSZ_RHIME

Accession

Primary (citable) accession number: Q59746

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)