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Protein
Submitted name:

3-alpha-hydroxysteroid dehydrogenase

Gene
N/A
Organism
Pseudomonas sp. B-0831
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691NAD; via carbonyl oxygenCombined sources
Binding sitei153 – 1531NADCombined sources
Binding sitei157 – 1571NADCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 133NADCombined sources
Nucleotide bindingi32 – 332NADCombined sources
Nucleotide bindingi41 – 422NADCombined sources
Nucleotide bindingi186 – 1883NADCombined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseImported

Keywords - Ligandi

NADCombined sources, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BRENDAi1.1.1.50. 5085.

Names & Taxonomyi

Protein namesi
Submitted name:
3-alpha-hydroxysteroid dehydrogenaseImported (EC:1.1.1.50Imported)
OrganismiPseudomonas sp. B-0831Imported
Taxonomic identifieri402890 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKNX-ray1.80A/B1-255[»]
ProteinModelPortaliQ59718.
SMRiQ59718. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59718.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIAITGSA SGIGAALKEL LARAGHTVIG IDRGQADIEA DLSTPGGRET
60 70 80 90 100
AVAAVLDRCG GVLDGLVCCA GVGVTAANSG LVVAVNYFGV SALLDGLAEA
110 120 130 140 150
LSRGQQPAAV IVGSIAATQP GAAELPMVEA MLAGDEARAI ELAEQQGQTH
160 170 180 190 200
LAYAGSKYAV TCLARRNVVD WAGRGVRLNV VAPGAVETPL LQASKADPRY
210 220 230 240 250
GESTRRFVAP LGRGSEPREV AEAIAFLLGP QASFIHGSVL FVDGGMDALM

RAKTF
Length:255
Mass (Da):25,893
Last modified:November 1, 1996 - v1
Checksum:iA407B4B6201FE088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50325 Genomic DNA. Translation: BAA08861.1.
PIRiJN0829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50325 Genomic DNA. Translation: BAA08861.1.
PIRiJN0829.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKNX-ray1.80A/B1-255[»]
ProteinModelPortaliQ59718.
SMRiQ59718. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.1.1.50. 5085.

Miscellaneous databases

EvolutionaryTraceiQ59718.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a Pseudomonas 3 alpha-hydroxysteroid dehydrogenase-encoding gene in Escherichia coli."
    Suzuki K., Ueda S., Sugiyama M., Imamura S.
    Gene 130:137-140(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B-0831Imported.
  2. Thiaucourt F.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B-0831Imported.
  3. "Apo- and holo-structures of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831. Loop-helix transition induced by coenzyme binding."
    Nakamura S., Oda M., Kataoka S., Ueda S., Uchiyama S., Yoshida T., Kobayashi Y., Ohkubo T.
    J. Biol. Chem. 281:31876-31884(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH NAD.

Entry informationi

Entry nameiQ59718_9PSED
AccessioniPrimary (citable) accession number: Q59718
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.