Reviewed, UniProtKB/Swiss-Prot Q59661 (DHSA_PARDE)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:

sdhA

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Succinate dehydrogenase flavoprotein subunit		PRO_0000158655
Regions
	Nucleotide binding	40 – 55	16	FAD By similarity
Sites
	Active site	289	1	Proton acceptor By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	257	1	Substrate By similarity
	Binding site	356	1	Substrate By similarity
	Binding site	401	1	Substrate By similarity
Amino acid modifications
	Modified residue	48	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59661-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E8446E4FB6D8F829	FASTA	600	65,616
	10 20 30 40 50 60 MAAYKYETHE YDVVVVGAGG AGLRATLGMA EQGLRTACVT KVFPTRSHTV AAQGGIAASL 70 80 90 100 110 120 SNMGPDNWQW HMYDTVKGSD WLGDTDAMEY LAREAPKAVY ELEHYGVPFS RTEEGKIYQR 130 140 150 160 170 180 PFGGHTTEFG EGPPVQRTCA AADRTGHAIL HTLYGQSLKE KAEFFIEYFA LDLIITDGAC 190 200 210 220 230 240 TGVVCWKLDD GTIHVFNAKM VVLATGGYGR AYFSATSAHT CTGDGGGMVA RAGLPLQDME 250 260 270 280 290 300 FVQFHPTGIY GSGCLITEGA RGEGGYLTNS EGERFMERYA PTYKDLASRD VVSRCITIEI 310 320 330 340 350 360 REGRGVGPHK DHMHLNLMHL PPESLAERLP GISESAKIFA GVDVTREPIP ILPTVHYNMG 370 380 390 400 410 420 GIPTNYWGEV LNPTQDNPDQ VFPGLMAVGE AGCASVHGAN RLGSNSLIDL VVFGRAAAIR 430 440 450 460 470 480 AGQVIDREAQ IPTTNKEQVD KALDRFDRIR NADGSVSTAD LRLEMQRTMQ ADAAVFRTDK 490 500 510 520 530 540 TLAEGVDKMR VIAGKLSDLK VTDRSLIWNS DLMETLELTN LMPNALATIV AAEARKESRG 550 560 570 580 590 600 AHAHEDYPER DDANWRKHSL AWIEGNDVKL AYRPVHLEPL TRQDEGGIDL KKIAPKARVY

References

[1]

"Cloning, sequencing, and expression of the succinate-ubiquinone oxidoreductase (SdhCDAB) operon from Paracoccus denitrificans." Dickins M.A., Dhawan T., Gunsalus R.P., Schroeder I., Cecchini G. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31902 Genomic DNA. Translation: AAA75177.1.
PIR	T46880.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
ModBase	Search...
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR011281. Succ_DHase_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_PARDE

Accession

Primary (citable) accession number: Q59661

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)