Q59653 (PYRB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	PA0402

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP-Rule MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP-Rule MF_00001
Subunit structure	Heterododecamer of 6 active PyrB subunits and 6 non-catalytic PyrC' subunits By similarity.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway 'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Aspartate carbamoyltransferase HAMAP-Rule MF_00001		PRO_0000113176
Experimental info
	Sequence conflict	206	1	R → A in AAA25976. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59653 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 2DC90450FA2E42E9	FASTA	334	36,629
	10 20 30 40 50 60 MPTDAKRPLQ LNDQGQLRHF ISLDGLPREL LTEILDTADS FLEVGARAVK KVPLLRGKTV 70 80 90 100 110 120 CNVFFENSTR TRTTFELAAQ RLSADVISLN VSTSSTSKGE TLTDTLRNLE AMAADMFVVR 130 140 150 160 170 180 HSDSGAAHFI AEHVSPNVAV INGGDGRHAH PTQGMLDMLT IRRHKGNFEQ LSVAIVGDIL 190 200 210 220 230 240 HSRVARSNML ALKTLGCPDI RVIAPRTLLP IGLEEQYGVR VFTNADEGLK DVDVVIMLRL 250 260 270 280 290 300 QRERMQGGLL PSEGEFFKLY GLTEKRLKLA KPDAIVMHPG PINRGVEIES AVADGAQSVI 310 320 330 LNQVTYGIAI RMAVLSMAMS GQNTQRQLEQ EDAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Vickrey J.F., Schurr M.J., Benjamin R.C., Cunin R., Shanley M.S., O'Donovan G.A. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19649 Genomic DNA. Translation: AAA25976.1. AE004091 Genomic DNA. Translation: AAG03791.1.
PIR	H83595.
RefSeq	NP_249093.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q59653.
SMR	Q59653. Positions 17-314.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA0402.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878267.
KEGG	pae:PA0402.
PATRIC	19835044. VBIPseAer58763_0423.
Organism-specific databases
PseudoCAP	PA0402.
Phylogenomic databases
eggNOG	COG0540.
KO	K00609.
OMA	LTIRQHK.
ProtClustDB	PRK00856.
Enzyme and pathway databases
UniPathway	UPA00070; UER00116.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr.
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR002082. Asp_carbamoyltransf. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_PSEAE

Accession

Primary (citable) accession number: Q59653

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program