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Protein

Nitric oxide reductase subunit B

Gene

norB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation (By similarity).By similarity

Catalytic activityi

Nitrous oxide + 2 ferricytochrome c + H2O = 2 nitric oxide + 2 ferrocytochrome c + 2 H+.

Pathwayi: nitrate reduction (denitrification)

This protein is involved in step 3 of the subpathway that synthesizes dinitrogen from nitrate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Nitric oxide reductase subunit B (norB)
  4. Nitrous-oxide reductase (nosZ)
This subpathway is part of the pathway nitrate reduction (denitrification), which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dinitrogen from nitrate, the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron (low-spin heme axial ligand)Curated
Metal bindingi207 – 2071Iron BCurated
Metal bindingi258 – 2581Iron BCurated
Metal bindingi259 – 2591Iron BCurated
Metal bindingi348 – 3481Iron (high-spin heme axial ligand)Curated
Metal bindingi350 – 3501Iron (low-spin heme axial ligand)Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00652; UER00708.

Protein family/group databases

TCDBi3.D.4.10.3. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide reductase subunit B (EC:1.7.2.5)
Alternative name(s):
NOR large subunit
Nitric oxide reductase cytochrome b subunit
Gene namesi
Name:norB
Ordered Locus Names:PA0524
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0524.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921HelicalSequence analysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence analysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence analysisAdd
BLAST
Transmembranei308 – 32821HelicalSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST
Transmembranei391 – 41121HelicalSequence analysisAdd
BLAST
Transmembranei434 – 45421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Nitric oxide reductase subunit BPRO_0000183472Add
BLAST

Proteomic databases

PaxDbiQ59647.

Interactioni

Subunit structurei

Heterodimer of cytochromes b (large subunit) and c (small subunit).

Protein-protein interaction databases

STRINGi208964.PA0524.

Structurei

Secondary structure

1
466
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 155Combined sources
Helixi16 – 4025Combined sources
Turni44 – 507Combined sources
Helixi53 – 8432Combined sources
Helixi91 – 11424Combined sources
Helixi117 – 1237Combined sources
Helixi126 – 1283Combined sources
Helixi141 – 16323Combined sources
Helixi169 – 18517Combined sources
Helixi186 – 1894Combined sources
Helixi195 – 21016Combined sources
Helixi213 – 23018Combined sources
Helixi234 – 25522Combined sources
Helixi256 – 2594Combined sources
Beta strandi261 – 2655Combined sources
Helixi268 – 27912Combined sources
Helixi281 – 29616Combined sources
Helixi306 – 32217Combined sources
Helixi324 – 3318Combined sources
Helixi333 – 3397Combined sources
Helixi343 – 36826Combined sources
Turni369 – 3746Combined sources
Helixi381 – 41636Combined sources
Turni421 – 4233Combined sources
Helixi427 – 4337Combined sources
Helixi435 – 45622Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O0RX-ray2.70B1-466[»]
3WFBX-ray2.70B1-466[»]
3WFCX-ray2.50B1-466[»]
3WFDX-ray2.30B1-466[»]
3WFEX-ray2.49B1-466[»]
ProteinModelPortaliQ59647.
SMRiQ59647. Positions 10-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DSQ. Bacteria.
COG3256. LUCA.
InParanoidiQ59647.
KOiK04561.
OMAiLDKMYWW.
PhylomeDBiQ59647.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSPNGSLKF ASQAVAKPYF VFALILFVGQ ILFGLIMGLQ YVVGDFLFPA
60 70 80 90 100
IPFNVARMVH TNLLIVWLLF GFMGAAYYLV PEESDCELYS PKLAWILFWV
110 120 130 140 150
FAAAGVLTIL GYLLVPYAGL ARLTGNELWP TMGREFLEQP TISKAGIVIV
160 170 180 190 200
ALGFLFNVGM TVLRGRKTAI SMVLMTGLIG LALLFLFSFY NPENLTRDKF
210 220 230 240 250
YWWWVVHLWV EGVWELIMGA ILAFVLVKIT GVDREVIEKW LYVIIAMALI
260 270 280 290 300
SGIIGTGHHY FWIGVPGYWL WLGSVFSALE PLPFFAMVLF AFNTINRRRR
310 320 330 340 350
RDYPNRAVAL WAMGTTVMAF LGAGVWGFMH TLAPVNYYTH GTQLTAAHGH
360 370 380 390 400
MAFYGAYAMI VMTIISYAMP RLRGIGEAMD NRSQVLEMWG FWLMTVAMVF
410 420 430 440 450
ITLFLSAAGV LQVWLQRMPA DGAAMTFMAT QDQLAIFYWL REGAGVVFLI
460
GLVAYLLSFR RGKAAA
Length:466
Mass (Da):52,336
Last modified:November 1, 1996 - v1
Checksum:iDFB379172C86D5B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38133 Genomic DNA. Translation: BAA07330.1.
AE004091 Genomic DNA. Translation: AAG03913.1.
PIRiS53713.
RefSeqiNP_249215.1. NC_002516.2.
WP_003113237.1. NZ_ASJY01000100.1.

Genome annotation databases

EnsemblBacteriaiAAG03913; AAG03913; PA0524.
GeneIDi882193.
KEGGipae:PA0524.
PATRICi19835306. VBIPseAer58763_0554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38133 Genomic DNA. Translation: BAA07330.1.
AE004091 Genomic DNA. Translation: AAG03913.1.
PIRiS53713.
RefSeqiNP_249215.1. NC_002516.2.
WP_003113237.1. NZ_ASJY01000100.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O0RX-ray2.70B1-466[»]
3WFBX-ray2.70B1-466[»]
3WFCX-ray2.50B1-466[»]
3WFDX-ray2.30B1-466[»]
3WFEX-ray2.49B1-466[»]
ProteinModelPortaliQ59647.
SMRiQ59647. Positions 10-459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0524.

Protein family/group databases

TCDBi3.D.4.10.3. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

PaxDbiQ59647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03913; AAG03913; PA0524.
GeneIDi882193.
KEGGipae:PA0524.
PATRICi19835306. VBIPseAer58763_0554.

Organism-specific databases

PseudoCAPiPA0524.

Phylogenomic databases

eggNOGiENOG4105DSQ. Bacteria.
COG3256. LUCA.
InParanoidiQ59647.
KOiK04561.
OMAiLDKMYWW.
PhylomeDBiQ59647.

Enzyme and pathway databases

UniPathwayiUPA00652; UER00708.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNORB_PSEAE
AccessioniPrimary (citable) accession number: Q59647
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.