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Reviewed, UniProtKB/Swiss-Prot Q59647 (NORB_PSEAE)

Last modified November 25, 2008. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nitric oxide reductase subunit B
    EC=1.7.99.7
Alternative name(s):
    Nitric oxide reductase cytochrome b subunit
    NOR large subunit
Gene names
Name: norB
Ordered Locus Names: PA0524
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation By similarity.

Catalytic activity

Nitrous oxide + acceptor + H(2)O = 2 nitric oxide + reduced acceptor.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 3/4.

Subunit structure

Heterodimer of cytochromes b (large subunit) and c (small subunit).

Subcellular location

Cell membrane; Multi-pass membrane proteinPotential.

Sequence similarities

Belongs to the heme-copper respiratory oxidase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Nitric oxide reductase subunit B
PRO_0000183472

Regions

Transmembrane19 – 3921 Potential
Transmembrane61 – 8121 Potential
Transmembrane95 – 11521 Potential
Transmembrane142 – 16221 Potential
Transmembrane169 – 18921 Potential
Transmembrane205 – 22521 Potential
Transmembrane243 – 26321 Potential
Transmembrane270 – 29021 Potential
Transmembrane308 – 32821 Potential
Transmembrane349 – 36921 Potential
Transmembrane391 – 41121 Potential
Transmembrane434 – 45421 Potential

Sites

Metal binding601Iron (low-spin heme axial ligand) Probable
Metal binding2071Iron B Probable
Metal binding2581Iron B Probable
Metal binding2591Iron B Probable
Metal binding3481Iron (high-spin heme axial ligand) Probable
Metal binding3501Iron (low-spin heme axial ligand) Probable

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Sequences

Sequence LengthMass (Da)Tools
Q59647-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DFB379172C86D5B7

FASTA46652,336
        10         20         30         40         50         60 
MMSPNGSLKF ASQAVAKPYF VFALILFVGQ ILFGLIMGLQ YVVGDFLFPA IPFNVARMVH 

        70         80         90        100        110        120 
TNLLIVWLLF GFMGAAYYLV PEESDCELYS PKLAWILFWV FAAAGVLTIL GYLLVPYAGL 

       130        140        150        160        170        180 
ARLTGNELWP TMGREFLEQP TISKAGIVIV ALGFLFNVGM TVLRGRKTAI SMVLMTGLIG 

       190        200        210        220        230        240 
LALLFLFSFY NPENLTRDKF YWWWVVHLWV EGVWELIMGA ILAFVLVKIT GVDREVIEKW 

       250        260        270        280        290        300 
LYVIIAMALI SGIIGTGHHY FWIGVPGYWL WLGSVFSALE PLPFFAMVLF AFNTINRRRR 

       310        320        330        340        350        360 
RDYPNRAVAL WAMGTTVMAF LGAGVWGFMH TLAPVNYYTH GTQLTAAHGH MAFYGAYAMI 

       370        380        390        400        410        420 
VMTIISYAMP RLRGIGEAMD NRSQVLEMWG FWLMTVAMVF ITLFLSAAGV LQVWLQRMPA 

       430        440        450        460 
DGAAMTFMAT QDQLAIFYWL REGAGVVFLI GLVAYLLSFR RGKAAA

« Hide

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Cross-references

Sequence databases

D38133 Genomic DNA. Translation: BAA07330.1.
AE004091 Genomic DNA. Translation: AAG03913.1.
PIRS53713.
RefSeqNP_249215.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID882193.
GenomeReviewsGene locus PA0524 in contig AE004091_GR.
KEGGpae:PA0524.
NMPDRfig|208964.1.peg.525.

Organism-specific databases

PseudoCAPPA0524.
CMRSearch...

Phylogenomic databases

HOGENOMQ59647.

Enzyme and pathway databases

BioCycPAER208964:PA0524-MON.

Family and domain databases

InterProIPR000883. COX1.
[Graphical view]
Gene3DG3DSA:1.20.210.10. COX1. 1 hit.
PfamPF00115. COX1. 1 hit.
[Graphical view]
PROSITEPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNORB_PSEAE
AccessionPrimary (citable) accession number: Q59647
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents