Reviewed, UniProtKB/Swiss-Prot Q59642 (LDHD_PEDAC)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:

ldhD

Organism

Pediococcus acidilactici

Taxonomic identifier

1254 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Pediococcus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has both D-lactate and D-glycerate dehydrogenase activities. Equally active on pyruvate and hydroxypyruvate.
Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	D-lactate dehydrogenase		PRO_0000075958
Regions
	Nucleotide binding	154 – 155	2	NAD By similarity
	Nucleotide binding	232 – 234	3	NAD By similarity
	Nucleotide binding	295 – 298	4	NAD By similarity
Sites
	Active site	234	1	By similarity
	Active site	263	1	By similarity
	Active site	295	1	Proton donor By similarity
	Binding site	258	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59642-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C2E6653EC661AEB8	FASTA	331	37,210
	10 20 30 40 50 60 MKIIAYGIRD DEKPYLDEWV TKNHIEVKAV PDLLDSSNID LAKDYDGVVA YQQKPYTADL 70 80 90 100 110 120 FDKMHEFGIH AFSLRNVGLD NVPADALKKN DIKISNVPAY SPRAIAELSV TQLLALLRKI 130 140 150 160 170 180 PEFEYKMAHG DYRWEPDIGL ELNQMTVGVI GTGRIGRAAI DIFKPFGAKV IAYDVFRNPA 190 200 210 220 230 240 LEKEGMYVDT LEELYQQANV ITLHVPALKD NYHMLDEKAF GQMQDGTFIL NFARGTLVDT 250 260 270 280 290 300 PALLKALDSG KVAGAALDTY ENEVGIFDVD HGDQPIDDPV FNDLMSRRNV MITPHAAFYT 310 320 330 RPAVKNMVQI ALDNNRDLIE KNSSKNEVKF E

References

[1]	"Pediococcus acidilactici ldhD gene: cloning, nucleotide sequence, and transcriptional analysis." Garmyn D., Ferain T., Bernard N., Hols P., Delplace B., Delcour J. J. Bacteriol. 177:3427-3437(1995) [PubMed: 7539419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DG302.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70925 Genomic DNA. Translation: CAA50275.1.
3D structure databases
SMR	Q59642. Positions 1-329.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.28. 1193.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_PEDAC

Accession

Primary (citable) accession number: Q59642

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)