Q59640 (CDSA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidate cytidylyltransferase
EC=2.7.7.41

Alternative name(s):
CDP-DAG synthase
CDP-DG synthase
CDP-diacylglycerol synthase
Short name=CDS
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
CTP:phosphatidate cytidylyltransferase

Gene names

Name:	cdsA
Synonyms:	cds
Ordered Locus Names:	PA3651

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDS family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Phosphatidate cytidylyltransferase		PRO_0000090744
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Transmembrane	251 – 271	21	Helical; Potential
Experimental info
	Sequence conflict	131 – 133	3	WPL → VAA in BAA09437. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59640 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 5025059C3F1A64C7	FASTA	271	28,856
	10 20 30 40 50 60 MLKQRIITAL VLLPIALGGF FLLEGAFFAL FIGAVVSLGA WEWARLAGYE QQFGRVAYAA 70 80 90 100 110 120 TVAVLMVALY HLPQLAGAVL LLALVWWTLA TVLVLTYPES VGYWGGRWRR LGMGLLILLP 130 140 150 160 170 180 AWQGLVLLKQ WPLANGLIIA VMVLVWGADI GAYFSGKAFG KRKLAPRVSP GKSWEGVYGG 190 200 210 220 230 240 LAASLAITLA VGLYRGWSLG ALLLALLGAA LVVFVSIVGD LTESMFKRQS GIKDSSNLLP 250 260 270 GHGGVLDRID SLTAAIPVFA ALLWAAGWGA P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Pseudomonas aeruginosa gene encoding CDP-diglyceride synthetase." Taguchi K., Fukutomi H., Kuroda A., Kato J., Ohtake H. Gene 172:165-166(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50811 Genomic DNA. Translation: BAA09437.1. AE004091 Genomic DNA. Translation: AAG07039.1.
PIR	F83188. JC4832.
RefSeq	NP_252341.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q59640.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA3651.
Protocols and materials databases
DNASU	880465.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880465.
KEGG	pae:PA3651.
PATRIC	19841925. VBIPseAer58763_3820.
Organism-specific databases
PseudoCAP	PA3651.
Phylogenomic databases
eggNOG	COG0575.
HOGENOM	HOG000006168.
KO	K00981.
OMA	WEWGRLN.
ProtClustDB	CLSK868131.
Enzyme and pathway databases
UniPathway	UPA00557; UER00614.
Family and domain databases
InterPro	IPR000374. PC_trans. [Graphical view]
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDSA_PSEAE

Accession

Primary (citable) accession number: Q59640

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program