Reviewed, UniProtKB/Swiss-Prot Q59640 (CDSA_PSEAE)

Names and origin

Protein names

Recommended name:
    Phosphatidate cytidylyltransferase
    EC=2.7.7.41
Alternative name(s):
    CDP-diglyceride pyrophosphorylase
    CDP-diglyceride synthase
    CDP-diacylglycerol synthase
      Short name=CDS
    CTP:phosphatidate cytidylyltransferase
    CDP-DG synthase
    CDP-DAG synthase

Gene names

Name:	cdsA
Synonyms:	cds
Ordered Locus Names:	PA3651

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDS family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphatidate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Phosphatidate cytidylyltransferase		PRO_0000090744
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	53 – 73	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	111 – 131	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	174 – 194	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	251 – 271	21	Potential
Experimental info
	Sequence conflict	131 – 133	3	WPL → VAA in BAA09437. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59640-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 5025059C3F1A64C7	FASTA	271	28,856
	10 20 30 40 50 60 MLKQRIITAL VLLPIALGGF FLLEGAFFAL FIGAVVSLGA WEWARLAGYE QQFGRVAYAA 70 80 90 100 110 120 TVAVLMVALY HLPQLAGAVL LLALVWWTLA TVLVLTYPES VGYWGGRWRR LGMGLLILLP 130 140 150 160 170 180 AWQGLVLLKQ WPLANGLIIA VMVLVWGADI GAYFSGKAFG KRKLAPRVSP GKSWEGVYGG 190 200 210 220 230 240 LAASLAITLA VGLYRGWSLG ALLLALLGAA LVVFVSIVGD LTESMFKRQS GIKDSSNLLP 250 260 270 GHGGVLDRID SLTAAIPVFA ALLWAAGWGA P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Pseudomonas aeruginosa gene encoding CDP-diglyceride synthetase." Taguchi K., Fukutomi H., Kuroda A., Kato J., Ohtake H. Gene 172:165-166(1996) [PubMed: 8654980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50811 Genomic DNA. Translation: BAA09437.1. AE004091 Genomic DNA. Translation: AAG07039.1.
PIR	F83188. JC4832.
RefSeq	NP_252341.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880465.
GenomeReviews	Gene locus PA3651 in contig AE004091_GR.
KEGG	pae:PA3651.
Organism-specific databases
PseudoCAP	PA3651.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732679.
OMA	WPLGNWL.
Enzyme and pathway databases
BioCyc	PAER208964:PA3651-MONOMER.
BRENDA	2.7.7.41. 354.
Family and domain databases
InterPro	IPR000374. PC_trans. [Graphical view]
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDSA_PSEAE

Accession

Primary (citable) accession number: Q59640

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	February 9, 2010
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)