Reviewed, UniProtKB/Swiss-Prot Q59639 (ALYA_PSEAL)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:

aly

Organism

Pseudomonas alginovora

Taxonomic identifier

37638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 7 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 233	210	Alginate lyase		PRO_0000024926

Sequences

	Sequence		Length	Mass (Da)
	Q59639-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6D0CC3AB733E6DDC	FASTA	233	25,873
	10 20 30 40 50 60 MKIISCKSII VSSLLALSAT ATAGSFNDIS WTLENEDNLP ETDASGCALK PSTSTSTSKT 70 80 90 100 110 120 FEFGLTDDSN CLDGKQRDEF KYQRRTGYNR LTGYFTIDGN YSDFNKMGVA QTHDHSTSDT 130 140 150 160 170 180 GVFSIYQVRK ENGSYIFGVQ GDSNYSNNGW SDHPQVKISL DTRYELIIKT NGLPNGNSYE 190 200 210 220 230 DANLYLDDVK IWSSSIEVGG EEKQYKKIGA YQLTGGEGEF HVKWDSVKLY TGK

References

[1]

"Cloning, sequencing and overexpression in Escherichia coli of the alginatelyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases." Chavagnat F., Duez C., Guinand M., Potin P., Barbeyron T., Henrissat B., Wallach J., Ghuysen J.-M. Biochem. J. 319:575-583(1996) [PubMed: 8912697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-28; 191-202 AND 208-222. Strain: XO17.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83679 Genomic DNA. Translation: CAA58650.1.
PIR	S72449.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.2.3. 275434.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ALYA_PSEAL

Accession

Primary (citable) accession number: Q59639

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)