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Q59637 (ODP1_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component
Short name=PDH E1 component
EC=1.2.4.1
Gene names
Name:aceE
Synonyms:aceA
Ordered Locus Names:PA5015
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length882 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 By similarity.

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Thiamine pyrophosphate By similarity.

Subunit structure

Homodimer By similarity. Makes part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Ontologies

Keywords
   Biological processGlycolysis
   LigandPyruvate
Thiamine pyrophosphate
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpyruvate dehydrogenase (acetyl-transferring) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 882882Pyruvate dehydrogenase E1 component
PRO_0000162247

Experimental info

Sequence conflict76 – 783IRS → DPLP in AAC45353. Ref.1
Sequence conflict149 – 1568EGRISEEQ → GRPHQRRNK in AAC45353. Ref.1
Sequence conflict167 – 1748NGLSSYPH → TACPPIRT in AAC45353. Ref.1
Sequence conflict3771Q → H in AAC45353. Ref.1
Sequence conflict3881G → V in AAC45353. Ref.1
Sequence conflict3921G → A in AAC45353. Ref.1
Sequence conflict408 – 4092Missing in AAC45353. Ref.1
Sequence conflict5171A → G in AAC45353. Ref.1
Sequence conflict6711Missing in AAC45353. Ref.1
Sequence conflict7401R → H in AAC45353. Ref.1
Sequence conflict744 – 7463GIG → DIV in AAC45353. Ref.1
Sequence conflict760 – 7656RDGLAV → HDDLTL in AAC45353. Ref.1
Sequence conflict788 – 7914GRRG → APSR in AAC45353. Ref.1
Sequence conflict8211Missing in AAC45353. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q59637 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 5FD387B8332E24D9

FASTA88299,564
        10         20         30         40         50         60 
MQDLDPVETQ EWLDALESVL DREGEDRAHY LMTRMGELAS RSGTQLPYAI TTPYRNTIPV 

        70         80         90        100        110        120 
THEARMPGDL FMERRIRSLV RWNALAMVMR ANKHDPDLGG HISTFASSAT LYDIGFNYFF 

       130        140        150        160        170        180 
QAPTDEHGGD LVFFQGHASP GVYARAFLEG RISEEQLENF RQEVDGNGLS SYPHPWLMPD 

       190        200        210        220        230        240 
FWQFPTVSMG LGPIQAIYQA RFMKYLESRG FIPAGKQKVW CFMGDGECDE PESLGAISLA 

       250        260        270        280        290        300 
GREKLDNLIF VINCNLQRLD GPVRGNAKII QELEGVFRGA EWNVNKVIWG RFWDPLFAKD 

       310        320        330        340        350        360 
TAGLLQQRMD EVIDGEYQNY KAKDGAYVRE HFFGARPELL EMVKDLSDEE IWKLNRGGHD 

       370        380        390        400        410        420 
PYKVYAAYHQ AVNHKGQPTV ILAKTIKGYG TGSGEAKNIA HNVKKVDVDS LRAFRDKFDI 

       430        440        450        460        470        480 
PVKDADLEKL PFYKPEEGSA EAKYLAERRA ALGGFMPVRR QKSMSVPVPP LETLKAMLDG 

       490        500        510        520        530        540 
SGDREISTTM AFVRIISQLV KDKELGPRIV PIVPDEARTF GMEGMFRQLG IYSSVGQLYE 

       550        560        570        580        590        600 
PVDKDQVMFY REDKKGQILE EGINEAGAMS SWIAAGTSYS THNQPMLPFY IFYSMFGFQR 

       610        620        630        640        650        660 
IGDLAWAAGD SRAHGFLIGG TAGRTTLNGE GLQHEDGHSH LLASTIPNCR TYDPTYAYEL 

       670        680        690        700        710        720 
AVIIREGSRQ MIEEQQDIFY YITVMNENYV QPAMPKGAEE GIIKGMYLLE EDKKEAAHHV 

       730        740        750        760        770        780 
QLLGSGTILR EVEEAAKLLR NDFGIGADVW SVPSFNELRR DGLAVERWNR LHPGQKPKQS 

       790        800        810        820        830        840 
YVEECLGGRR GPVIASTDYM KLYAEQIRQW VPSKEYKVLG TDGFGRSDSR KKLRNFFEVD 

       850        860        870        880 
RHWVVLAALE ALADRGDIEP KVVAEAIAKY GIDPEKRNPL DC

« Hide

References

« Hide 'large scale' references
[1]"Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa."
Rae J.L., Cutfield J.F., Lamont I.L.
J. Bacteriol. 179:3561-3571(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PAO.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U47920 Genomic DNA. Translation: AAC45353.1.
AE004091 Genomic DNA. Translation: AAG08400.1.
PIRG83018.
RefSeqNP_253702.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ59637.
SMRQ59637. Positions 54-879.
ModBaseSearch...

Protein-protein interaction databases

STRING208964.PA5015.

Proteomic databases

PRIDEQ59637.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID881326.
KEGGpae:PA5015.
PATRIC19844853. VBIPseAer58763_5255.

Organism-specific databases

PseudoCAPPA5015.

Phylogenomic databases

eggNOGCOG2609.
HOGENOMHOG000115215.
KOK00163.
OMANLIFTIS.
ProtClustDBPRK09405.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
InterProIPR004660. 2-oxoA_DH_E1.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR015941. Transketolase-like_C.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF00456. Transketolase_N. 2 hits.
[Graphical view]
PIRSFPIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMSSF52922. Transketo_C_like. 1 hit.
TIGRFAMsTIGR00759. aceE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODP1_PSEAE
AccessionPrimary (citable) accession number: Q59637
Secondary accession number(s): Q9HUF3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info